Cargando…
The Microvillar and Solitary Chemosensory Cells as the Novel Targets of Infection of SARS-CoV-2 in Syrian Golden Hamsters
Patients infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of coronavirus disease 2019, suffer from respiratory and non-respiratory symptoms. Among these symptoms, the loss of smell has attracted considerable attention. The objectives of this study were...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8402700/ https://www.ncbi.nlm.nih.gov/pubmed/34452517 http://dx.doi.org/10.3390/v13081653 |
_version_ | 1783745853764141056 |
---|---|
author | Seo, Jin-Seok Yoon, Sun-Woo Hwang, Seung-Hyeon Nam, Sung-Min Nahm, Sang-Soep Jeong, Jei-Hyun Lee, Jiho Youn, Ha-Na Kim, Jun-Beom Kim, Woosuk |
author_facet | Seo, Jin-Seok Yoon, Sun-Woo Hwang, Seung-Hyeon Nam, Sung-Min Nahm, Sang-Soep Jeong, Jei-Hyun Lee, Jiho Youn, Ha-Na Kim, Jun-Beom Kim, Woosuk |
author_sort | Seo, Jin-Seok |
collection | PubMed |
description | Patients infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of coronavirus disease 2019, suffer from respiratory and non-respiratory symptoms. Among these symptoms, the loss of smell has attracted considerable attention. The objectives of this study were to determine which cells are infected, what happens in the olfactory system after viral infection, and how these pathologic changes contribute to olfactory loss. For this purpose, Syrian golden hamsters were used. First, we verified the olfactory structures in the nasal cavity of Syrian golden hamsters, namely the main olfactory epithelium, the vomeronasal organ, and their cellular components. Second, we found angiotensin-converting enzyme 2 expression, a receptor protein of SARS-CoV-2, in both structures and infections of supporting, microvillar, and solitary chemosensory cells. Third, we observed pathological changes in the infected epithelium, including reduced thickness of the mucus layer, detached epithelia, indistinct layers of epithelia, infiltration of inflammatory cells, and apoptotic cells in the overall layers. We concluded that a structurally and functionally altered microenvironment influences olfactory function. We observed the regeneration of the damaged epithelium, and found multilayers of basal cells, indicating that they were activated and proliferating to reconstitute the injured epithelium. |
format | Online Article Text |
id | pubmed-8402700 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-84027002021-08-29 The Microvillar and Solitary Chemosensory Cells as the Novel Targets of Infection of SARS-CoV-2 in Syrian Golden Hamsters Seo, Jin-Seok Yoon, Sun-Woo Hwang, Seung-Hyeon Nam, Sung-Min Nahm, Sang-Soep Jeong, Jei-Hyun Lee, Jiho Youn, Ha-Na Kim, Jun-Beom Kim, Woosuk Viruses Article Patients infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of coronavirus disease 2019, suffer from respiratory and non-respiratory symptoms. Among these symptoms, the loss of smell has attracted considerable attention. The objectives of this study were to determine which cells are infected, what happens in the olfactory system after viral infection, and how these pathologic changes contribute to olfactory loss. For this purpose, Syrian golden hamsters were used. First, we verified the olfactory structures in the nasal cavity of Syrian golden hamsters, namely the main olfactory epithelium, the vomeronasal organ, and their cellular components. Second, we found angiotensin-converting enzyme 2 expression, a receptor protein of SARS-CoV-2, in both structures and infections of supporting, microvillar, and solitary chemosensory cells. Third, we observed pathological changes in the infected epithelium, including reduced thickness of the mucus layer, detached epithelia, indistinct layers of epithelia, infiltration of inflammatory cells, and apoptotic cells in the overall layers. We concluded that a structurally and functionally altered microenvironment influences olfactory function. We observed the regeneration of the damaged epithelium, and found multilayers of basal cells, indicating that they were activated and proliferating to reconstitute the injured epithelium. MDPI 2021-08-20 /pmc/articles/PMC8402700/ /pubmed/34452517 http://dx.doi.org/10.3390/v13081653 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Seo, Jin-Seok Yoon, Sun-Woo Hwang, Seung-Hyeon Nam, Sung-Min Nahm, Sang-Soep Jeong, Jei-Hyun Lee, Jiho Youn, Ha-Na Kim, Jun-Beom Kim, Woosuk The Microvillar and Solitary Chemosensory Cells as the Novel Targets of Infection of SARS-CoV-2 in Syrian Golden Hamsters |
title | The Microvillar and Solitary Chemosensory Cells as the Novel Targets of Infection of SARS-CoV-2 in Syrian Golden Hamsters |
title_full | The Microvillar and Solitary Chemosensory Cells as the Novel Targets of Infection of SARS-CoV-2 in Syrian Golden Hamsters |
title_fullStr | The Microvillar and Solitary Chemosensory Cells as the Novel Targets of Infection of SARS-CoV-2 in Syrian Golden Hamsters |
title_full_unstemmed | The Microvillar and Solitary Chemosensory Cells as the Novel Targets of Infection of SARS-CoV-2 in Syrian Golden Hamsters |
title_short | The Microvillar and Solitary Chemosensory Cells as the Novel Targets of Infection of SARS-CoV-2 in Syrian Golden Hamsters |
title_sort | microvillar and solitary chemosensory cells as the novel targets of infection of sars-cov-2 in syrian golden hamsters |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8402700/ https://www.ncbi.nlm.nih.gov/pubmed/34452517 http://dx.doi.org/10.3390/v13081653 |
work_keys_str_mv | AT seojinseok themicrovillarandsolitarychemosensorycellsasthenoveltargetsofinfectionofsarscov2insyriangoldenhamsters AT yoonsunwoo themicrovillarandsolitarychemosensorycellsasthenoveltargetsofinfectionofsarscov2insyriangoldenhamsters AT hwangseunghyeon themicrovillarandsolitarychemosensorycellsasthenoveltargetsofinfectionofsarscov2insyriangoldenhamsters AT namsungmin themicrovillarandsolitarychemosensorycellsasthenoveltargetsofinfectionofsarscov2insyriangoldenhamsters AT nahmsangsoep themicrovillarandsolitarychemosensorycellsasthenoveltargetsofinfectionofsarscov2insyriangoldenhamsters AT jeongjeihyun themicrovillarandsolitarychemosensorycellsasthenoveltargetsofinfectionofsarscov2insyriangoldenhamsters AT leejiho themicrovillarandsolitarychemosensorycellsasthenoveltargetsofinfectionofsarscov2insyriangoldenhamsters AT younhana themicrovillarandsolitarychemosensorycellsasthenoveltargetsofinfectionofsarscov2insyriangoldenhamsters AT kimjunbeom themicrovillarandsolitarychemosensorycellsasthenoveltargetsofinfectionofsarscov2insyriangoldenhamsters AT kimwoosuk themicrovillarandsolitarychemosensorycellsasthenoveltargetsofinfectionofsarscov2insyriangoldenhamsters AT seojinseok microvillarandsolitarychemosensorycellsasthenoveltargetsofinfectionofsarscov2insyriangoldenhamsters AT yoonsunwoo microvillarandsolitarychemosensorycellsasthenoveltargetsofinfectionofsarscov2insyriangoldenhamsters AT hwangseunghyeon microvillarandsolitarychemosensorycellsasthenoveltargetsofinfectionofsarscov2insyriangoldenhamsters AT namsungmin microvillarandsolitarychemosensorycellsasthenoveltargetsofinfectionofsarscov2insyriangoldenhamsters AT nahmsangsoep microvillarandsolitarychemosensorycellsasthenoveltargetsofinfectionofsarscov2insyriangoldenhamsters AT jeongjeihyun microvillarandsolitarychemosensorycellsasthenoveltargetsofinfectionofsarscov2insyriangoldenhamsters AT leejiho microvillarandsolitarychemosensorycellsasthenoveltargetsofinfectionofsarscov2insyriangoldenhamsters AT younhana microvillarandsolitarychemosensorycellsasthenoveltargetsofinfectionofsarscov2insyriangoldenhamsters AT kimjunbeom microvillarandsolitarychemosensorycellsasthenoveltargetsofinfectionofsarscov2insyriangoldenhamsters AT kimwoosuk microvillarandsolitarychemosensorycellsasthenoveltargetsofinfectionofsarscov2insyriangoldenhamsters |