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Molecular Epidemiology and Characterization of Picobirnavirus in Wild Deer and Cattle from Australia: Evidence of Genogroup I and II in the Upper Respiratory Tract

Picobirnaviruses (PBVs) have been detected in several species of animals worldwide; however, data pertaining to their presence in Australian wild and domestic animals are limited. Although PBVs are mostly found in faecal samples, their detection in blood and respiratory tract samples raises question...

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Autores principales: Huaman, Jose L., Pacioni, Carlo, Sarker, Subir, Doyle, Mark, Forsyth, David M., Pople, Anthony, Hampton, Jordan O., Carvalho, Teresa G., Helbig, Karla J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8402760/
https://www.ncbi.nlm.nih.gov/pubmed/34452357
http://dx.doi.org/10.3390/v13081492
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author Huaman, Jose L.
Pacioni, Carlo
Sarker, Subir
Doyle, Mark
Forsyth, David M.
Pople, Anthony
Hampton, Jordan O.
Carvalho, Teresa G.
Helbig, Karla J.
author_facet Huaman, Jose L.
Pacioni, Carlo
Sarker, Subir
Doyle, Mark
Forsyth, David M.
Pople, Anthony
Hampton, Jordan O.
Carvalho, Teresa G.
Helbig, Karla J.
author_sort Huaman, Jose L.
collection PubMed
description Picobirnaviruses (PBVs) have been detected in several species of animals worldwide; however, data pertaining to their presence in Australian wild and domestic animals are limited. Although PBVs are mostly found in faecal samples, their detection in blood and respiratory tract samples raises questions concerning their tropism and pathogenicity. We report here PBV detection in wild deer and cattle from southeastern Australia. Through metagenomics, the presence of PBV genogroups I (GI) and II (GII) were detected in deer serum and plasma. Molecular epidemiology studies targeting the partial RNA-dependent RNA polymerase gene were performed in a wide range of specimens (serum, faeces, spleen, lung, nasal swabs, and trachea) collected from wild deer and cattle, with PCR amplification obtained in all specimen types except lung and spleen. Our results reveal the predominance of GI and concomitant detection of both genogroups in wild deer and cattle. In concordance with other studies, the detected GI sequences displayed high genetic diversity, however in contrast, GII sequences clustered into three distinct clades. Detection of both genogroups in the upper respiratory tract (trachea and nasal swab) of deer in the present study gives more evidence about the respiratory tract tropism of PBV. Although much remains unknown about the epidemiology and tropism of PBVs, our study suggests a wide distribution of these viruses in southeastern Australia.
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spelling pubmed-84027602021-08-29 Molecular Epidemiology and Characterization of Picobirnavirus in Wild Deer and Cattle from Australia: Evidence of Genogroup I and II in the Upper Respiratory Tract Huaman, Jose L. Pacioni, Carlo Sarker, Subir Doyle, Mark Forsyth, David M. Pople, Anthony Hampton, Jordan O. Carvalho, Teresa G. Helbig, Karla J. Viruses Article Picobirnaviruses (PBVs) have been detected in several species of animals worldwide; however, data pertaining to their presence in Australian wild and domestic animals are limited. Although PBVs are mostly found in faecal samples, their detection in blood and respiratory tract samples raises questions concerning their tropism and pathogenicity. We report here PBV detection in wild deer and cattle from southeastern Australia. Through metagenomics, the presence of PBV genogroups I (GI) and II (GII) were detected in deer serum and plasma. Molecular epidemiology studies targeting the partial RNA-dependent RNA polymerase gene were performed in a wide range of specimens (serum, faeces, spleen, lung, nasal swabs, and trachea) collected from wild deer and cattle, with PCR amplification obtained in all specimen types except lung and spleen. Our results reveal the predominance of GI and concomitant detection of both genogroups in wild deer and cattle. In concordance with other studies, the detected GI sequences displayed high genetic diversity, however in contrast, GII sequences clustered into three distinct clades. Detection of both genogroups in the upper respiratory tract (trachea and nasal swab) of deer in the present study gives more evidence about the respiratory tract tropism of PBV. Although much remains unknown about the epidemiology and tropism of PBVs, our study suggests a wide distribution of these viruses in southeastern Australia. MDPI 2021-07-29 /pmc/articles/PMC8402760/ /pubmed/34452357 http://dx.doi.org/10.3390/v13081492 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Huaman, Jose L.
Pacioni, Carlo
Sarker, Subir
Doyle, Mark
Forsyth, David M.
Pople, Anthony
Hampton, Jordan O.
Carvalho, Teresa G.
Helbig, Karla J.
Molecular Epidemiology and Characterization of Picobirnavirus in Wild Deer and Cattle from Australia: Evidence of Genogroup I and II in the Upper Respiratory Tract
title Molecular Epidemiology and Characterization of Picobirnavirus in Wild Deer and Cattle from Australia: Evidence of Genogroup I and II in the Upper Respiratory Tract
title_full Molecular Epidemiology and Characterization of Picobirnavirus in Wild Deer and Cattle from Australia: Evidence of Genogroup I and II in the Upper Respiratory Tract
title_fullStr Molecular Epidemiology and Characterization of Picobirnavirus in Wild Deer and Cattle from Australia: Evidence of Genogroup I and II in the Upper Respiratory Tract
title_full_unstemmed Molecular Epidemiology and Characterization of Picobirnavirus in Wild Deer and Cattle from Australia: Evidence of Genogroup I and II in the Upper Respiratory Tract
title_short Molecular Epidemiology and Characterization of Picobirnavirus in Wild Deer and Cattle from Australia: Evidence of Genogroup I and II in the Upper Respiratory Tract
title_sort molecular epidemiology and characterization of picobirnavirus in wild deer and cattle from australia: evidence of genogroup i and ii in the upper respiratory tract
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8402760/
https://www.ncbi.nlm.nih.gov/pubmed/34452357
http://dx.doi.org/10.3390/v13081492
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