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Prevalence of Hantaviruses Harbored by Murid Rodents in Northwestern Ukraine and Discovery of a Novel Puumala Virus Strain

In Europe, two species of hantaviruses, Puumala orthohantavirus (PUUV) and Dobrava orthohantavirus (DOBV), cause hemorrhagic fever with renal syndrome in humans. The rodent reservoirs for these viruses are common throughout Ukraine, and hence, the goal of this study was to identify the species and s...

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Autores principales: Williams, Evan P., Taylor, Mariah K., Demchyshyna, Iryna, Nebogatkin, Igor, Nesterova, Olena, Khuda, Iryna, Chernenko, Lyudmyla, Hluzd, Oleksandra A., Kutseva, Vira V., Glass, Gregory E., Yanko, Nataliia, Jonsson, Colleen B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8402871/
https://www.ncbi.nlm.nih.gov/pubmed/34452504
http://dx.doi.org/10.3390/v13081640
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author Williams, Evan P.
Taylor, Mariah K.
Demchyshyna, Iryna
Nebogatkin, Igor
Nesterova, Olena
Khuda, Iryna
Chernenko, Lyudmyla
Hluzd, Oleksandra A.
Kutseva, Vira V.
Glass, Gregory E.
Yanko, Nataliia
Jonsson, Colleen B.
author_facet Williams, Evan P.
Taylor, Mariah K.
Demchyshyna, Iryna
Nebogatkin, Igor
Nesterova, Olena
Khuda, Iryna
Chernenko, Lyudmyla
Hluzd, Oleksandra A.
Kutseva, Vira V.
Glass, Gregory E.
Yanko, Nataliia
Jonsson, Colleen B.
author_sort Williams, Evan P.
collection PubMed
description In Europe, two species of hantaviruses, Puumala orthohantavirus (PUUV) and Dobrava orthohantavirus (DOBV), cause hemorrhagic fever with renal syndrome in humans. The rodent reservoirs for these viruses are common throughout Ukraine, and hence, the goal of this study was to identify the species and strains of hantaviruses circulating in this region. We conducted surveillance of small rodent populations in a rural region in northwestern Ukraine approximately 30 km from Poland. From the 424 small mammals captured, we identified nine species, of which the most abundant were Myodes glareolus, the bank vole (45%); Apodemus flavicollis, the yellow-necked mouse (29%); and Apodemus agrarius, the striped field mouse (14.6%) Using an indirect immunofluorescence assay, 15.7%, 20.5%, and 33.9% of the sera from M. glareolus, A. glareolus, and A. flavicollis were positive for hantaviral antibodies, respectively. Additionally, we detected antibodies to the hantaviral antigen in one Microtus arvalis, one Mus musculus, and one Sorex minutus. We screened the lung tissue for hantaviral RNA using next-generation sequencing and identified PUUV sequences in 25 small mammals, including 23 M. glareolus, 1 M. musculus, and 1 A. flavicollis, but we were unable to detect DOBV sequences in any of our A. agrarius specimens. The percent identity matrix and Bayesian phylogenetic analyses of the S-segment of PUUV from 14 M. glareolus lungs suggest the highest similarity (92–95% nucleotide or 99–100% amino acid) with the Latvian lineage. This new genetic information will contribute to future molecular surveillance of human cases in Ukraine.
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spelling pubmed-84028712021-08-29 Prevalence of Hantaviruses Harbored by Murid Rodents in Northwestern Ukraine and Discovery of a Novel Puumala Virus Strain Williams, Evan P. Taylor, Mariah K. Demchyshyna, Iryna Nebogatkin, Igor Nesterova, Olena Khuda, Iryna Chernenko, Lyudmyla Hluzd, Oleksandra A. Kutseva, Vira V. Glass, Gregory E. Yanko, Nataliia Jonsson, Colleen B. Viruses Article In Europe, two species of hantaviruses, Puumala orthohantavirus (PUUV) and Dobrava orthohantavirus (DOBV), cause hemorrhagic fever with renal syndrome in humans. The rodent reservoirs for these viruses are common throughout Ukraine, and hence, the goal of this study was to identify the species and strains of hantaviruses circulating in this region. We conducted surveillance of small rodent populations in a rural region in northwestern Ukraine approximately 30 km from Poland. From the 424 small mammals captured, we identified nine species, of which the most abundant were Myodes glareolus, the bank vole (45%); Apodemus flavicollis, the yellow-necked mouse (29%); and Apodemus agrarius, the striped field mouse (14.6%) Using an indirect immunofluorescence assay, 15.7%, 20.5%, and 33.9% of the sera from M. glareolus, A. glareolus, and A. flavicollis were positive for hantaviral antibodies, respectively. Additionally, we detected antibodies to the hantaviral antigen in one Microtus arvalis, one Mus musculus, and one Sorex minutus. We screened the lung tissue for hantaviral RNA using next-generation sequencing and identified PUUV sequences in 25 small mammals, including 23 M. glareolus, 1 M. musculus, and 1 A. flavicollis, but we were unable to detect DOBV sequences in any of our A. agrarius specimens. The percent identity matrix and Bayesian phylogenetic analyses of the S-segment of PUUV from 14 M. glareolus lungs suggest the highest similarity (92–95% nucleotide or 99–100% amino acid) with the Latvian lineage. This new genetic information will contribute to future molecular surveillance of human cases in Ukraine. MDPI 2021-08-18 /pmc/articles/PMC8402871/ /pubmed/34452504 http://dx.doi.org/10.3390/v13081640 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Williams, Evan P.
Taylor, Mariah K.
Demchyshyna, Iryna
Nebogatkin, Igor
Nesterova, Olena
Khuda, Iryna
Chernenko, Lyudmyla
Hluzd, Oleksandra A.
Kutseva, Vira V.
Glass, Gregory E.
Yanko, Nataliia
Jonsson, Colleen B.
Prevalence of Hantaviruses Harbored by Murid Rodents in Northwestern Ukraine and Discovery of a Novel Puumala Virus Strain
title Prevalence of Hantaviruses Harbored by Murid Rodents in Northwestern Ukraine and Discovery of a Novel Puumala Virus Strain
title_full Prevalence of Hantaviruses Harbored by Murid Rodents in Northwestern Ukraine and Discovery of a Novel Puumala Virus Strain
title_fullStr Prevalence of Hantaviruses Harbored by Murid Rodents in Northwestern Ukraine and Discovery of a Novel Puumala Virus Strain
title_full_unstemmed Prevalence of Hantaviruses Harbored by Murid Rodents in Northwestern Ukraine and Discovery of a Novel Puumala Virus Strain
title_short Prevalence of Hantaviruses Harbored by Murid Rodents in Northwestern Ukraine and Discovery of a Novel Puumala Virus Strain
title_sort prevalence of hantaviruses harbored by murid rodents in northwestern ukraine and discovery of a novel puumala virus strain
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8402871/
https://www.ncbi.nlm.nih.gov/pubmed/34452504
http://dx.doi.org/10.3390/v13081640
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