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Characterization of Membrane-Associated Progesterone Receptor Component-2 (MAPRC2) from Trichinella spiralis and Its Interaction with Progesterone and Mifepristone

Trichinellosis is a foodborne zoonotic disease caused by Trichinella spp., including Trichinella spiralis. In the present study, T. spiralis membrane-associated progesterone receptor component-2 (Ts-MAPRC2) gene was cloned and characterized using protein sequencing analysis. Furthermore, the express...

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Autores principales: Aleem, Muhammad Tahir, Shi, Jiawen, Yu, Zhengqing, Wen, Zhaohai, Zhang, Yang, Liang, Meng, Lakho, Shakeel Ahmed, Haseeb, Muhammad, Ali, Haider, Hassan, Muhammad Waqas, Song, Xiaokai, Li, Xiangrui, Xu, Lixin, Yan, Ruofeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8402905/
https://www.ncbi.nlm.nih.gov/pubmed/34452060
http://dx.doi.org/10.3390/vaccines9080934
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author Aleem, Muhammad Tahir
Shi, Jiawen
Yu, Zhengqing
Wen, Zhaohai
Zhang, Yang
Liang, Meng
Lakho, Shakeel Ahmed
Haseeb, Muhammad
Ali, Haider
Hassan, Muhammad Waqas
Song, Xiaokai
Li, Xiangrui
Xu, Lixin
Yan, Ruofeng
author_facet Aleem, Muhammad Tahir
Shi, Jiawen
Yu, Zhengqing
Wen, Zhaohai
Zhang, Yang
Liang, Meng
Lakho, Shakeel Ahmed
Haseeb, Muhammad
Ali, Haider
Hassan, Muhammad Waqas
Song, Xiaokai
Li, Xiangrui
Xu, Lixin
Yan, Ruofeng
author_sort Aleem, Muhammad Tahir
collection PubMed
description Trichinellosis is a foodborne zoonotic disease caused by Trichinella spp., including Trichinella spiralis. In the present study, T. spiralis membrane-associated progesterone receptor component-2 (Ts-MAPRC2) gene was cloned and characterized using protein sequencing analysis. Furthermore, the expression, purification, immunoblot assay, binding ability with progesterone antibody, and immunofluorescence assay were performed. A direct effect of progesterone (P4) and mifepristone (RU486) on the Ts-MAPRC2 gene was determined using in vitro cell culture that showed different expression levels at all developmental stages (muscle larvae (ML), female adult worm (F-AL), male adult worm (M-AL), and newborn larvae (NBL)). Subsequently, the in vitro phenotypic effects of P4, RU486, and rTs-MAPRC2-Ab on F-AL and ML stages were measured. Later, the in vivo phenotypic effect and relative mRNA expression of mifepristone on the F-AL stage were studied. Our results revealed that the Ts-MAPRC2 gene is critical to maintaining pregnancy in the female adult worm (F-AL) of T. spiralis. The 300 ng/mL of P4 and 100 ng/mL of RU486 showed downregulation of the Ts-MAPRC2 gene in F-AL (p ≤ 0.05). This plays an important role in abortion and possibly decreases the worm burden of T. spiralis in the host. Only 30 ng/mL P4 showed significant upregulation in F-AL (p ≤ 0.05). The current study provides new insights regarding the antihormone (P4 and RU486) drug design and vaccine therapy of recombinant (rTs-MAPRC2) protein as well as their combined effects to control T. spiralis infection.
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spelling pubmed-84029052021-08-29 Characterization of Membrane-Associated Progesterone Receptor Component-2 (MAPRC2) from Trichinella spiralis and Its Interaction with Progesterone and Mifepristone Aleem, Muhammad Tahir Shi, Jiawen Yu, Zhengqing Wen, Zhaohai Zhang, Yang Liang, Meng Lakho, Shakeel Ahmed Haseeb, Muhammad Ali, Haider Hassan, Muhammad Waqas Song, Xiaokai Li, Xiangrui Xu, Lixin Yan, Ruofeng Vaccines (Basel) Article Trichinellosis is a foodborne zoonotic disease caused by Trichinella spp., including Trichinella spiralis. In the present study, T. spiralis membrane-associated progesterone receptor component-2 (Ts-MAPRC2) gene was cloned and characterized using protein sequencing analysis. Furthermore, the expression, purification, immunoblot assay, binding ability with progesterone antibody, and immunofluorescence assay were performed. A direct effect of progesterone (P4) and mifepristone (RU486) on the Ts-MAPRC2 gene was determined using in vitro cell culture that showed different expression levels at all developmental stages (muscle larvae (ML), female adult worm (F-AL), male adult worm (M-AL), and newborn larvae (NBL)). Subsequently, the in vitro phenotypic effects of P4, RU486, and rTs-MAPRC2-Ab on F-AL and ML stages were measured. Later, the in vivo phenotypic effect and relative mRNA expression of mifepristone on the F-AL stage were studied. Our results revealed that the Ts-MAPRC2 gene is critical to maintaining pregnancy in the female adult worm (F-AL) of T. spiralis. The 300 ng/mL of P4 and 100 ng/mL of RU486 showed downregulation of the Ts-MAPRC2 gene in F-AL (p ≤ 0.05). This plays an important role in abortion and possibly decreases the worm burden of T. spiralis in the host. Only 30 ng/mL P4 showed significant upregulation in F-AL (p ≤ 0.05). The current study provides new insights regarding the antihormone (P4 and RU486) drug design and vaccine therapy of recombinant (rTs-MAPRC2) protein as well as their combined effects to control T. spiralis infection. MDPI 2021-08-23 /pmc/articles/PMC8402905/ /pubmed/34452060 http://dx.doi.org/10.3390/vaccines9080934 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Aleem, Muhammad Tahir
Shi, Jiawen
Yu, Zhengqing
Wen, Zhaohai
Zhang, Yang
Liang, Meng
Lakho, Shakeel Ahmed
Haseeb, Muhammad
Ali, Haider
Hassan, Muhammad Waqas
Song, Xiaokai
Li, Xiangrui
Xu, Lixin
Yan, Ruofeng
Characterization of Membrane-Associated Progesterone Receptor Component-2 (MAPRC2) from Trichinella spiralis and Its Interaction with Progesterone and Mifepristone
title Characterization of Membrane-Associated Progesterone Receptor Component-2 (MAPRC2) from Trichinella spiralis and Its Interaction with Progesterone and Mifepristone
title_full Characterization of Membrane-Associated Progesterone Receptor Component-2 (MAPRC2) from Trichinella spiralis and Its Interaction with Progesterone and Mifepristone
title_fullStr Characterization of Membrane-Associated Progesterone Receptor Component-2 (MAPRC2) from Trichinella spiralis and Its Interaction with Progesterone and Mifepristone
title_full_unstemmed Characterization of Membrane-Associated Progesterone Receptor Component-2 (MAPRC2) from Trichinella spiralis and Its Interaction with Progesterone and Mifepristone
title_short Characterization of Membrane-Associated Progesterone Receptor Component-2 (MAPRC2) from Trichinella spiralis and Its Interaction with Progesterone and Mifepristone
title_sort characterization of membrane-associated progesterone receptor component-2 (maprc2) from trichinella spiralis and its interaction with progesterone and mifepristone
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8402905/
https://www.ncbi.nlm.nih.gov/pubmed/34452060
http://dx.doi.org/10.3390/vaccines9080934
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