Cargando…

Generation and Evaluation of Recombinant Thermostable Newcastle Disease Virus Expressing the HA of H9N2 Avian Influenza Virus

H9N2 avian influenza virus (AIV) has become endemic in many countries, causing great economic losses when co-infected with other pathogens. So far, several live vaccines based on Newcastle disease virus (NDV) vectors expressing influenza hemagglutinin (HA) have been developed. However, the thermosta...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhang, Xiaorong, Bo, Zongyi, Meng, Chenchen, Chen, Yin, Zhang, Chengcheng, Cao, Yongzhong, Wu, Yantao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8402907/
https://www.ncbi.nlm.nih.gov/pubmed/34452473
http://dx.doi.org/10.3390/v13081606
_version_ 1783745903675310080
author Zhang, Xiaorong
Bo, Zongyi
Meng, Chenchen
Chen, Yin
Zhang, Chengcheng
Cao, Yongzhong
Wu, Yantao
author_facet Zhang, Xiaorong
Bo, Zongyi
Meng, Chenchen
Chen, Yin
Zhang, Chengcheng
Cao, Yongzhong
Wu, Yantao
author_sort Zhang, Xiaorong
collection PubMed
description H9N2 avian influenza virus (AIV) has become endemic in many countries, causing great economic losses when co-infected with other pathogens. So far, several live vaccines based on Newcastle disease virus (NDV) vectors expressing influenza hemagglutinin (HA) have been developed. However, the thermostable recombinant NDV is rarely reported. In this study, using a thermostable NDV rAHR09 strain as the vector, three recombinant NDVs expressing native HA, chimeric HA ectodomain with transmembrane domain/C-terminal cytoplasmic tail domain from fusion protein of NDV, and HA ectodomain were generated, designated rAHR09-HA, rAHR09-HAF, and rAHR09-HAE. The MDT value of three recombinant NDVs was above 120 h, their ICPI value was about 0.03, and the recombinant NDVs were still infectious when treated for 100 min under 56 °C, which demonstrated that the recombinant NDVs kept the lentogenic and thermostable nature of rAHR09. The immunization data showed that rAHR09-HA and rAHR09-HAF induced a higher HI antibody titer against H9N2 AIV and NDV. After being challenged with H9N2 AIV, the rAHR09-HA and rAHR09-HAF could significantly reduce the virus shedding in cloacal and tracheal swab samples. Our results suggest that rAHR09-HA and rAHR09-HAF might be vaccine candidates against H9N2 AIV.
format Online
Article
Text
id pubmed-8402907
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-84029072021-08-29 Generation and Evaluation of Recombinant Thermostable Newcastle Disease Virus Expressing the HA of H9N2 Avian Influenza Virus Zhang, Xiaorong Bo, Zongyi Meng, Chenchen Chen, Yin Zhang, Chengcheng Cao, Yongzhong Wu, Yantao Viruses Article H9N2 avian influenza virus (AIV) has become endemic in many countries, causing great economic losses when co-infected with other pathogens. So far, several live vaccines based on Newcastle disease virus (NDV) vectors expressing influenza hemagglutinin (HA) have been developed. However, the thermostable recombinant NDV is rarely reported. In this study, using a thermostable NDV rAHR09 strain as the vector, three recombinant NDVs expressing native HA, chimeric HA ectodomain with transmembrane domain/C-terminal cytoplasmic tail domain from fusion protein of NDV, and HA ectodomain were generated, designated rAHR09-HA, rAHR09-HAF, and rAHR09-HAE. The MDT value of three recombinant NDVs was above 120 h, their ICPI value was about 0.03, and the recombinant NDVs were still infectious when treated for 100 min under 56 °C, which demonstrated that the recombinant NDVs kept the lentogenic and thermostable nature of rAHR09. The immunization data showed that rAHR09-HA and rAHR09-HAF induced a higher HI antibody titer against H9N2 AIV and NDV. After being challenged with H9N2 AIV, the rAHR09-HA and rAHR09-HAF could significantly reduce the virus shedding in cloacal and tracheal swab samples. Our results suggest that rAHR09-HA and rAHR09-HAF might be vaccine candidates against H9N2 AIV. MDPI 2021-08-13 /pmc/articles/PMC8402907/ /pubmed/34452473 http://dx.doi.org/10.3390/v13081606 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhang, Xiaorong
Bo, Zongyi
Meng, Chenchen
Chen, Yin
Zhang, Chengcheng
Cao, Yongzhong
Wu, Yantao
Generation and Evaluation of Recombinant Thermostable Newcastle Disease Virus Expressing the HA of H9N2 Avian Influenza Virus
title Generation and Evaluation of Recombinant Thermostable Newcastle Disease Virus Expressing the HA of H9N2 Avian Influenza Virus
title_full Generation and Evaluation of Recombinant Thermostable Newcastle Disease Virus Expressing the HA of H9N2 Avian Influenza Virus
title_fullStr Generation and Evaluation of Recombinant Thermostable Newcastle Disease Virus Expressing the HA of H9N2 Avian Influenza Virus
title_full_unstemmed Generation and Evaluation of Recombinant Thermostable Newcastle Disease Virus Expressing the HA of H9N2 Avian Influenza Virus
title_short Generation and Evaluation of Recombinant Thermostable Newcastle Disease Virus Expressing the HA of H9N2 Avian Influenza Virus
title_sort generation and evaluation of recombinant thermostable newcastle disease virus expressing the ha of h9n2 avian influenza virus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8402907/
https://www.ncbi.nlm.nih.gov/pubmed/34452473
http://dx.doi.org/10.3390/v13081606
work_keys_str_mv AT zhangxiaorong generationandevaluationofrecombinantthermostablenewcastlediseasevirusexpressingthehaofh9n2avianinfluenzavirus
AT bozongyi generationandevaluationofrecombinantthermostablenewcastlediseasevirusexpressingthehaofh9n2avianinfluenzavirus
AT mengchenchen generationandevaluationofrecombinantthermostablenewcastlediseasevirusexpressingthehaofh9n2avianinfluenzavirus
AT chenyin generationandevaluationofrecombinantthermostablenewcastlediseasevirusexpressingthehaofh9n2avianinfluenzavirus
AT zhangchengcheng generationandevaluationofrecombinantthermostablenewcastlediseasevirusexpressingthehaofh9n2avianinfluenzavirus
AT caoyongzhong generationandevaluationofrecombinantthermostablenewcastlediseasevirusexpressingthehaofh9n2avianinfluenzavirus
AT wuyantao generationandevaluationofrecombinantthermostablenewcastlediseasevirusexpressingthehaofh9n2avianinfluenzavirus