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Transcriptome Analysis Reveals the Molecular Immunological Characteristics of Lesions in Patients with Halo Nevi When Compared to Stable Vitiligo, Normal Nevocytic Nevi and Cutaneous Melanoma

BACKGROUND: Given their similar appearance and histology, halo nevi (HN) were considered as a type of vitiligo. However, whether HN have stronger immune response than stable vitiligo (VL) remains unclear. In addition, the molecular alterations in HN compared with normal nevocytic nevi (NN) and prima...

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Autores principales: Pan, Chun, Shang, Jingzhe, Jiang, Haiqin, Shi, Ying, Zhang, Wenyue, Xiong, Jingshu, Mei, Youming, Long, Siyu, Ge, Gai, Wang, Zhenzhen, Wu, Ziwei, Wang, Hongsheng, Wu, Aiping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8403033/
https://www.ncbi.nlm.nih.gov/pubmed/34466016
http://dx.doi.org/10.2147/JIR.S321672
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author Pan, Chun
Shang, Jingzhe
Jiang, Haiqin
Shi, Ying
Zhang, Wenyue
Xiong, Jingshu
Mei, Youming
Long, Siyu
Ge, Gai
Wang, Zhenzhen
Wu, Ziwei
Wang, Hongsheng
Wu, Aiping
author_facet Pan, Chun
Shang, Jingzhe
Jiang, Haiqin
Shi, Ying
Zhang, Wenyue
Xiong, Jingshu
Mei, Youming
Long, Siyu
Ge, Gai
Wang, Zhenzhen
Wu, Ziwei
Wang, Hongsheng
Wu, Aiping
author_sort Pan, Chun
collection PubMed
description BACKGROUND: Given their similar appearance and histology, halo nevi (HN) were considered as a type of vitiligo. However, whether HN have stronger immune response than stable vitiligo (VL) remains unclear. In addition, the molecular alterations in HN compared with normal nevocytic nevi (NN) and primary cutaneous melanoma (MM) must be determined. This study aimed to systematically characterize the molecular immunological features of HN. METHODS: Skin samples from patients with HN, VL, NN, and MM were obtained with informed consent. Each of the four groups underwent transcriptome sequencing and data analysis were for pairwise comparison. Quantitative real-time PCR (RT-qPCR) was conducted to confirm the transcriptional expression of some differentially expressed genes (DEGs) that were closely related to immunity. RESULTS: A total of 441 and 1507 DEGs were found in the HN/NN and HN/MM groups, respectively. Compared with those of VL, HN lesions contained 162 up-regulated DEGs and 12 down-regulated DEGs. Bioinformatics analysis showed that the up-regulated genes in HN were substantially enriched in immune response, immune deficiency, and immune rejection; biological stimulation (virus, bacteria); and proliferation and activation of immune cells. Immune cell composition analysis also confirmed high expression levels of multiple immunocytes in HN. CONCLUSION: The molecular immune mechanisms of HN and VL were similar, but the immune activity of HN was stronger than that of VL. Innate and adaptive immunity were involved in the pathogenesis and progression of HN and VL.
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spelling pubmed-84030332021-08-30 Transcriptome Analysis Reveals the Molecular Immunological Characteristics of Lesions in Patients with Halo Nevi When Compared to Stable Vitiligo, Normal Nevocytic Nevi and Cutaneous Melanoma Pan, Chun Shang, Jingzhe Jiang, Haiqin Shi, Ying Zhang, Wenyue Xiong, Jingshu Mei, Youming Long, Siyu Ge, Gai Wang, Zhenzhen Wu, Ziwei Wang, Hongsheng Wu, Aiping J Inflamm Res Original Research BACKGROUND: Given their similar appearance and histology, halo nevi (HN) were considered as a type of vitiligo. However, whether HN have stronger immune response than stable vitiligo (VL) remains unclear. In addition, the molecular alterations in HN compared with normal nevocytic nevi (NN) and primary cutaneous melanoma (MM) must be determined. This study aimed to systematically characterize the molecular immunological features of HN. METHODS: Skin samples from patients with HN, VL, NN, and MM were obtained with informed consent. Each of the four groups underwent transcriptome sequencing and data analysis were for pairwise comparison. Quantitative real-time PCR (RT-qPCR) was conducted to confirm the transcriptional expression of some differentially expressed genes (DEGs) that were closely related to immunity. RESULTS: A total of 441 and 1507 DEGs were found in the HN/NN and HN/MM groups, respectively. Compared with those of VL, HN lesions contained 162 up-regulated DEGs and 12 down-regulated DEGs. Bioinformatics analysis showed that the up-regulated genes in HN were substantially enriched in immune response, immune deficiency, and immune rejection; biological stimulation (virus, bacteria); and proliferation and activation of immune cells. Immune cell composition analysis also confirmed high expression levels of multiple immunocytes in HN. CONCLUSION: The molecular immune mechanisms of HN and VL were similar, but the immune activity of HN was stronger than that of VL. Innate and adaptive immunity were involved in the pathogenesis and progression of HN and VL. Dove 2021-08-24 /pmc/articles/PMC8403033/ /pubmed/34466016 http://dx.doi.org/10.2147/JIR.S321672 Text en © 2021 Pan et al. https://creativecommons.org/licenses/by-nc/3.0/This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/ (https://creativecommons.org/licenses/by-nc/3.0/) ). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Pan, Chun
Shang, Jingzhe
Jiang, Haiqin
Shi, Ying
Zhang, Wenyue
Xiong, Jingshu
Mei, Youming
Long, Siyu
Ge, Gai
Wang, Zhenzhen
Wu, Ziwei
Wang, Hongsheng
Wu, Aiping
Transcriptome Analysis Reveals the Molecular Immunological Characteristics of Lesions in Patients with Halo Nevi When Compared to Stable Vitiligo, Normal Nevocytic Nevi and Cutaneous Melanoma
title Transcriptome Analysis Reveals the Molecular Immunological Characteristics of Lesions in Patients with Halo Nevi When Compared to Stable Vitiligo, Normal Nevocytic Nevi and Cutaneous Melanoma
title_full Transcriptome Analysis Reveals the Molecular Immunological Characteristics of Lesions in Patients with Halo Nevi When Compared to Stable Vitiligo, Normal Nevocytic Nevi and Cutaneous Melanoma
title_fullStr Transcriptome Analysis Reveals the Molecular Immunological Characteristics of Lesions in Patients with Halo Nevi When Compared to Stable Vitiligo, Normal Nevocytic Nevi and Cutaneous Melanoma
title_full_unstemmed Transcriptome Analysis Reveals the Molecular Immunological Characteristics of Lesions in Patients with Halo Nevi When Compared to Stable Vitiligo, Normal Nevocytic Nevi and Cutaneous Melanoma
title_short Transcriptome Analysis Reveals the Molecular Immunological Characteristics of Lesions in Patients with Halo Nevi When Compared to Stable Vitiligo, Normal Nevocytic Nevi and Cutaneous Melanoma
title_sort transcriptome analysis reveals the molecular immunological characteristics of lesions in patients with halo nevi when compared to stable vitiligo, normal nevocytic nevi and cutaneous melanoma
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8403033/
https://www.ncbi.nlm.nih.gov/pubmed/34466016
http://dx.doi.org/10.2147/JIR.S321672
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