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In vitro and in vivo biological performance of hydroxyapatite from fish waste

The aim of this study was to evaluate biocompatibility of hydroxyapatite (HAP) from fish waste using in vitro and in vivo assays. Fish samples (whitemouth croaker - Micropogonias furnieri) from the biowaste was used as HAP source. Pre-osteoblastic MC3T3-E1 cells were used in vitro study. In addition...

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Autores principales: Prado, João Paulo dos Santos, Yamamura, Hirochi, Magri, Angela Maria Paiva, Ruiz, Pedro Luiz Muniz, Prado, José Lucas dos Santos, Rennó, Ana Claudia Muniz, Ribeiro, Daniel Araki, Granito, Renata Neves
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8403112/
https://www.ncbi.nlm.nih.gov/pubmed/34453621
http://dx.doi.org/10.1007/s10856-021-06591-x
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author Prado, João Paulo dos Santos
Yamamura, Hirochi
Magri, Angela Maria Paiva
Ruiz, Pedro Luiz Muniz
Prado, José Lucas dos Santos
Rennó, Ana Claudia Muniz
Ribeiro, Daniel Araki
Granito, Renata Neves
author_facet Prado, João Paulo dos Santos
Yamamura, Hirochi
Magri, Angela Maria Paiva
Ruiz, Pedro Luiz Muniz
Prado, José Lucas dos Santos
Rennó, Ana Claudia Muniz
Ribeiro, Daniel Araki
Granito, Renata Neves
author_sort Prado, João Paulo dos Santos
collection PubMed
description The aim of this study was to evaluate biocompatibility of hydroxyapatite (HAP) from fish waste using in vitro and in vivo assays. Fish samples (whitemouth croaker - Micropogonias furnieri) from the biowaste was used as HAP source. Pre-osteoblastic MC3T3-E1 cells were used in vitro study. In addition, bone defects were artificially created in rat calvaria and filled with HAP in vivo. The results demonstrated that HAP reduced cytotoxicity in pre-osteoblast cells after 3 and 6 days following HAP exposure. DNA concentration was lower in the HAP group after 6 days. Quantitative RT-PCR did not show any significant differences (p > 0.05) between groups. In vivo study revealed that bone defects filled with HAP pointed out moderate chronic inflammatory cells with slight proliferation of blood vessels after 7 and 15 days. Chronic inflammatory infiltrate was absent after 30 days of HAP exposure. There was also a decrease in the amount of biomaterial, being followed by newly formed bone tissue. All experimental groups also demonstrated strong RUNX-2 immoexpression in the granulation tissue as well as in cells in close contact with biomaterial. The number of osteoblasts inside the defect area was lower in the HAP group when compared to control group after 7 days post-implantation. Similarly, the osteoblast surface as well as the percentage of bone surface was higher in control group when compared with HAP group after 7 days post-implantation. Taken together, HAP from fish waste is a promising possibility that should be explored more carefully by tissue-engineering or biotechnology. [Image: see text]
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spelling pubmed-84031122021-09-15 In vitro and in vivo biological performance of hydroxyapatite from fish waste Prado, João Paulo dos Santos Yamamura, Hirochi Magri, Angela Maria Paiva Ruiz, Pedro Luiz Muniz Prado, José Lucas dos Santos Rennó, Ana Claudia Muniz Ribeiro, Daniel Araki Granito, Renata Neves J Mater Sci Mater Med Biocompatibility Studies The aim of this study was to evaluate biocompatibility of hydroxyapatite (HAP) from fish waste using in vitro and in vivo assays. Fish samples (whitemouth croaker - Micropogonias furnieri) from the biowaste was used as HAP source. Pre-osteoblastic MC3T3-E1 cells were used in vitro study. In addition, bone defects were artificially created in rat calvaria and filled with HAP in vivo. The results demonstrated that HAP reduced cytotoxicity in pre-osteoblast cells after 3 and 6 days following HAP exposure. DNA concentration was lower in the HAP group after 6 days. Quantitative RT-PCR did not show any significant differences (p > 0.05) between groups. In vivo study revealed that bone defects filled with HAP pointed out moderate chronic inflammatory cells with slight proliferation of blood vessels after 7 and 15 days. Chronic inflammatory infiltrate was absent after 30 days of HAP exposure. There was also a decrease in the amount of biomaterial, being followed by newly formed bone tissue. All experimental groups also demonstrated strong RUNX-2 immoexpression in the granulation tissue as well as in cells in close contact with biomaterial. The number of osteoblasts inside the defect area was lower in the HAP group when compared to control group after 7 days post-implantation. Similarly, the osteoblast surface as well as the percentage of bone surface was higher in control group when compared with HAP group after 7 days post-implantation. Taken together, HAP from fish waste is a promising possibility that should be explored more carefully by tissue-engineering or biotechnology. [Image: see text] Springer US 2021-08-28 2021 /pmc/articles/PMC8403112/ /pubmed/34453621 http://dx.doi.org/10.1007/s10856-021-06591-x Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Biocompatibility Studies
Prado, João Paulo dos Santos
Yamamura, Hirochi
Magri, Angela Maria Paiva
Ruiz, Pedro Luiz Muniz
Prado, José Lucas dos Santos
Rennó, Ana Claudia Muniz
Ribeiro, Daniel Araki
Granito, Renata Neves
In vitro and in vivo biological performance of hydroxyapatite from fish waste
title In vitro and in vivo biological performance of hydroxyapatite from fish waste
title_full In vitro and in vivo biological performance of hydroxyapatite from fish waste
title_fullStr In vitro and in vivo biological performance of hydroxyapatite from fish waste
title_full_unstemmed In vitro and in vivo biological performance of hydroxyapatite from fish waste
title_short In vitro and in vivo biological performance of hydroxyapatite from fish waste
title_sort in vitro and in vivo biological performance of hydroxyapatite from fish waste
topic Biocompatibility Studies
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8403112/
https://www.ncbi.nlm.nih.gov/pubmed/34453621
http://dx.doi.org/10.1007/s10856-021-06591-x
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