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Recombinant expression of Barnase in Escherichia coli and its application in plasmid purification

BACKGROUND: The use of bovine-origin ribonucleases has been part of the standard protocol for plasmid DNA purification. As the field of gene therapy now enters the clinical stage, such enzymes need to be phased out or alternative purification protocols need to be developed to ensure product safety a...

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Detalles Bibliográficos
Autores principales:	Shankar, Ram, Schäffer, Nina, Schmeer, Marco, Risse, Joe Max, Friehs, Karl, Schleef, Martin
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2021
Materias:	Research
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8403359/ https://www.ncbi.nlm.nih.gov/pubmed/34454498 http://dx.doi.org/10.1186/s12934-021-01642-y

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