A protocol to study bacteriophage adaptation to new hosts

A general protocol for the experimental assessment of bacteriophage adaptation to new hosts is described. We use as a model system the lytic phage T7 and an engineered E. coli strain modified to hamper the recruitment of a known proviral factor. Our protocol includes steps of phage amplification, pl...

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Detalles Bibliográficos
Autores principales: Luzon-Hidalgo, Raquel, Risso, Valeria A., Delgado, Asuncion, Ibarra-Molero, Beatriz, Sanchez-Ruiz, Jose M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8403759/
https://www.ncbi.nlm.nih.gov/pubmed/34485945
http://dx.doi.org/10.1016/j.xpro.2021.100784

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8403759/
https://www.ncbi.nlm.nih.gov/pubmed/34485945
http://dx.doi.org/10.1016/j.xpro.2021.100784

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