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A protocol to study bacteriophage adaptation to new hosts
A general protocol for the experimental assessment of bacteriophage adaptation to new hosts is described. We use as a model system the lytic phage T7 and an engineered E. coli strain modified to hamper the recruitment of a known proviral factor. Our protocol includes steps of phage amplification, pl...
Autores principales: | Luzon-Hidalgo, Raquel, Risso, Valeria A., Delgado, Asuncion, Ibarra-Molero, Beatriz, Sanchez-Ruiz, Jose M. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8403759/ https://www.ncbi.nlm.nih.gov/pubmed/34485945 http://dx.doi.org/10.1016/j.xpro.2021.100784 |
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