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Assessing the protective effects of cryptotanshinone on CoCl(2)-induced hypoxia in RPE cells
The development of several retinal diseases is closely related to hypoxia. As a component of the Traditional Chinese medicine Salvia miltiorrhiza, the effects of cryptotanshinone (CT) on retinal cells under hypoxic conditions are not well understood. The aim of the present study was to explore how C...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8404095/ https://www.ncbi.nlm.nih.gov/pubmed/34435647 http://dx.doi.org/10.3892/mmr.2021.12379 |
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author | Gu, Yu Liu, Weiming Liu, Gaoqin Li, Xin Lu, Peirong |
author_facet | Gu, Yu Liu, Weiming Liu, Gaoqin Li, Xin Lu, Peirong |
author_sort | Gu, Yu |
collection | PubMed |
description | The development of several retinal diseases is closely related to hypoxia. As a component of the Traditional Chinese medicine Salvia miltiorrhiza, the effects of cryptotanshinone (CT) on retinal cells under hypoxic conditions are not well understood. The aim of the present study was to explore how CT exerted its protective effects on retinal pigment epithelium (RPE) cells under hypoxic conditions induced by cobalt chloride (CoCl(2)). The effects of CT were investigated using a Cell Counting Kit-8 assay, Annexin V-FITC/PI staining, reverse transcription-quantitative PCR and western blotting in ARPE-19 cells. CT (10 and 20 µM) reduced the CoCl(2)-induced increase in vascular endothelial growth factor expression and hypoxia-inducible transcription factor-1α expression in ARPE-19 cells. Additionally, CT alleviated hypoxia-induced apoptosis by regulating Bcl-2 and Bax protein expression. CT treatment also reduced the increase in the mRNA levels of IL-6, IL-1β and TNF-α induced by CoCl(2). In summary, CT may protect RPE cells against apoptosis and inflammation in CoCl(2)-induced hypoxia, and these results warrant further in vivo study into its value as a drug for treating hypoxic eye diseases. |
format | Online Article Text |
id | pubmed-8404095 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-84040952021-08-31 Assessing the protective effects of cryptotanshinone on CoCl(2)-induced hypoxia in RPE cells Gu, Yu Liu, Weiming Liu, Gaoqin Li, Xin Lu, Peirong Mol Med Rep Articles The development of several retinal diseases is closely related to hypoxia. As a component of the Traditional Chinese medicine Salvia miltiorrhiza, the effects of cryptotanshinone (CT) on retinal cells under hypoxic conditions are not well understood. The aim of the present study was to explore how CT exerted its protective effects on retinal pigment epithelium (RPE) cells under hypoxic conditions induced by cobalt chloride (CoCl(2)). The effects of CT were investigated using a Cell Counting Kit-8 assay, Annexin V-FITC/PI staining, reverse transcription-quantitative PCR and western blotting in ARPE-19 cells. CT (10 and 20 µM) reduced the CoCl(2)-induced increase in vascular endothelial growth factor expression and hypoxia-inducible transcription factor-1α expression in ARPE-19 cells. Additionally, CT alleviated hypoxia-induced apoptosis by regulating Bcl-2 and Bax protein expression. CT treatment also reduced the increase in the mRNA levels of IL-6, IL-1β and TNF-α induced by CoCl(2). In summary, CT may protect RPE cells against apoptosis and inflammation in CoCl(2)-induced hypoxia, and these results warrant further in vivo study into its value as a drug for treating hypoxic eye diseases. D.A. Spandidos 2021-10 2021-08-20 /pmc/articles/PMC8404095/ /pubmed/34435647 http://dx.doi.org/10.3892/mmr.2021.12379 Text en Copyright: © Gu et al. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Gu, Yu Liu, Weiming Liu, Gaoqin Li, Xin Lu, Peirong Assessing the protective effects of cryptotanshinone on CoCl(2)-induced hypoxia in RPE cells |
title | Assessing the protective effects of cryptotanshinone on CoCl(2)-induced hypoxia in RPE cells |
title_full | Assessing the protective effects of cryptotanshinone on CoCl(2)-induced hypoxia in RPE cells |
title_fullStr | Assessing the protective effects of cryptotanshinone on CoCl(2)-induced hypoxia in RPE cells |
title_full_unstemmed | Assessing the protective effects of cryptotanshinone on CoCl(2)-induced hypoxia in RPE cells |
title_short | Assessing the protective effects of cryptotanshinone on CoCl(2)-induced hypoxia in RPE cells |
title_sort | assessing the protective effects of cryptotanshinone on cocl(2)-induced hypoxia in rpe cells |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8404095/ https://www.ncbi.nlm.nih.gov/pubmed/34435647 http://dx.doi.org/10.3892/mmr.2021.12379 |
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