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CD146 controls the quality of clinical grade mesenchymal stem cells from human dental pulp

BACKGROUND: Human mesenchymal stem cells from dental pulp (hMSC-DP), including dental pulp stem cells from permanent teeth and exfoliated deciduous teeth, possess unique MSC characteristics such as expression of specific surface molecules and a high proliferation rate. Since hMSC-DP have been applie...

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Autores principales: Ma, Lan, Huang, Zhiqing, Wu, Di, Kou, Xiaoxing, Mao, Xueli, Shi, Songtao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8404346/
https://www.ncbi.nlm.nih.gov/pubmed/34461987
http://dx.doi.org/10.1186/s13287-021-02559-4
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author Ma, Lan
Huang, Zhiqing
Wu, Di
Kou, Xiaoxing
Mao, Xueli
Shi, Songtao
author_facet Ma, Lan
Huang, Zhiqing
Wu, Di
Kou, Xiaoxing
Mao, Xueli
Shi, Songtao
author_sort Ma, Lan
collection PubMed
description BACKGROUND: Human mesenchymal stem cells from dental pulp (hMSC-DP), including dental pulp stem cells from permanent teeth and exfoliated deciduous teeth, possess unique MSC characteristics such as expression of specific surface molecules and a high proliferation rate. Since hMSC-DP have been applied in numerous clinical studies, it is necessary to establish criteria to evaluate their potency for cell-based therapies. METHODS: We compared stem cell properties of hMSC-DP at passages 5, 10 and 20 under serum (SE) and serum-free (SF) culture conditions. Cell morphology, proliferation capacity, chromosomal stability, surface phenotypic profiles, differentiation and immunoregulation ability were evaluated. In addition, we assessed surface molecule that regulates hMSC-DP proliferation and immunomodulation. RESULTS: hMSC-DP exhibited a decrease in proliferation rate and differentiation potential, as well as a reduced expression of CD146 when cultured under continuous passage conditions. SF culture conditions failed to alter surface marker expression, chromosome stability or proliferation rate when compared to SE culture. SF-cultured hMSC-DP were able to differentiate into osteogenic, adipogenic and neural cells, and displayed the capacity to regulate immune responses. Notably, the expression level of CD146 showed a positive correlation with proliferation, differentiation, and immunomodulation, suggesting that CD146 can serve as a surface molecule to evaluate the potency of hMSC-DP. Mechanistically, we found that CD146 regulates proliferation and immunomodulation of hMSC-DP through the ERK/p-ERK pathway. CONCLUSION: This study indicates that SF-cultured hMSC-DP are appropriate for producing clinical-grade cells. CD146 is a functional surface molecule to assess the potency of hMSC-DP. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13287-021-02559-4.
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spelling pubmed-84043462021-08-31 CD146 controls the quality of clinical grade mesenchymal stem cells from human dental pulp Ma, Lan Huang, Zhiqing Wu, Di Kou, Xiaoxing Mao, Xueli Shi, Songtao Stem Cell Res Ther Research BACKGROUND: Human mesenchymal stem cells from dental pulp (hMSC-DP), including dental pulp stem cells from permanent teeth and exfoliated deciduous teeth, possess unique MSC characteristics such as expression of specific surface molecules and a high proliferation rate. Since hMSC-DP have been applied in numerous clinical studies, it is necessary to establish criteria to evaluate their potency for cell-based therapies. METHODS: We compared stem cell properties of hMSC-DP at passages 5, 10 and 20 under serum (SE) and serum-free (SF) culture conditions. Cell morphology, proliferation capacity, chromosomal stability, surface phenotypic profiles, differentiation and immunoregulation ability were evaluated. In addition, we assessed surface molecule that regulates hMSC-DP proliferation and immunomodulation. RESULTS: hMSC-DP exhibited a decrease in proliferation rate and differentiation potential, as well as a reduced expression of CD146 when cultured under continuous passage conditions. SF culture conditions failed to alter surface marker expression, chromosome stability or proliferation rate when compared to SE culture. SF-cultured hMSC-DP were able to differentiate into osteogenic, adipogenic and neural cells, and displayed the capacity to regulate immune responses. Notably, the expression level of CD146 showed a positive correlation with proliferation, differentiation, and immunomodulation, suggesting that CD146 can serve as a surface molecule to evaluate the potency of hMSC-DP. Mechanistically, we found that CD146 regulates proliferation and immunomodulation of hMSC-DP through the ERK/p-ERK pathway. CONCLUSION: This study indicates that SF-cultured hMSC-DP are appropriate for producing clinical-grade cells. CD146 is a functional surface molecule to assess the potency of hMSC-DP. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13287-021-02559-4. BioMed Central 2021-08-30 /pmc/articles/PMC8404346/ /pubmed/34461987 http://dx.doi.org/10.1186/s13287-021-02559-4 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Ma, Lan
Huang, Zhiqing
Wu, Di
Kou, Xiaoxing
Mao, Xueli
Shi, Songtao
CD146 controls the quality of clinical grade mesenchymal stem cells from human dental pulp
title CD146 controls the quality of clinical grade mesenchymal stem cells from human dental pulp
title_full CD146 controls the quality of clinical grade mesenchymal stem cells from human dental pulp
title_fullStr CD146 controls the quality of clinical grade mesenchymal stem cells from human dental pulp
title_full_unstemmed CD146 controls the quality of clinical grade mesenchymal stem cells from human dental pulp
title_short CD146 controls the quality of clinical grade mesenchymal stem cells from human dental pulp
title_sort cd146 controls the quality of clinical grade mesenchymal stem cells from human dental pulp
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8404346/
https://www.ncbi.nlm.nih.gov/pubmed/34461987
http://dx.doi.org/10.1186/s13287-021-02559-4
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