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Chemical composition and antibacterial activity of Berberis vulgaris (barberry) against bacteria associated with caries
OBJECTIVES: The aim of this in‐vitro study was to determine the antimicrobial capacity of a Berberis vulgaris plant extract on the bacteria being associated with caries including, Streptococcus mutans, S. sobrinus, S. sanguinis, S. salivaris and Lactobacillus rhamnosus. MATERIAL AND METHODS: Chlorhe...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8404507/ https://www.ncbi.nlm.nih.gov/pubmed/33325156 http://dx.doi.org/10.1002/cre2.379 |
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author | Kazemipoor, Maryam Fadaei Tehrani, Pooya Zandi, Hengameh Golvardi Yazdi, Reza |
author_facet | Kazemipoor, Maryam Fadaei Tehrani, Pooya Zandi, Hengameh Golvardi Yazdi, Reza |
author_sort | Kazemipoor, Maryam |
collection | PubMed |
description | OBJECTIVES: The aim of this in‐vitro study was to determine the antimicrobial capacity of a Berberis vulgaris plant extract on the bacteria being associated with caries including, Streptococcus mutans, S. sobrinus, S. sanguinis, S. salivaris and Lactobacillus rhamnosus. MATERIAL AND METHODS: Chlorhexidine 2% (CHX) mouthwash and ampicillin (10 μg/disk) were applied as positive control groups. Inhibition zone, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) related to stem, leaf and fruit of B. vulgaris plant were recorded for every five bacteria. Data were analyzed using SPSS ver. 22, one‐way ANOVA repeated measure and post hoc Tukey statistical test. The significance level was set at p < 0.05. RESULTS: There were no significant differences between the antimicrobial capacity of the positive controls and the extract from the stem and fruit of B. vulgaris (p > 0.05). The MIC values of the extract from the stem were significantly lower against S. sobrinus (64 μg/ml) and L. rhamnosus (128 μg/ml). The MIC value of the extract against S. mutans was significantly lower in the fruit group (64 μg/mL). The MBC value of the extract against S. sobrinus and L. rhamnosus was significantly lower in the stem group (128 μg/ml). The MBC value against S. mutans was significantly lower in the fruit group (128 μg/ml). CONCLUSIONS: The results showed that CHX and B. vulgaris plant extract have similar antimicrobial activity against bacteria being associated with caries. Therefore, B. vulgaris, which shows antibacterial capacity, could be considered for further investigation as a safe, phytotherapeutic mouthwash to prevent dental caries. |
format | Online Article Text |
id | pubmed-8404507 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-84045072021-09-03 Chemical composition and antibacterial activity of Berberis vulgaris (barberry) against bacteria associated with caries Kazemipoor, Maryam Fadaei Tehrani, Pooya Zandi, Hengameh Golvardi Yazdi, Reza Clin Exp Dent Res Original Articles OBJECTIVES: The aim of this in‐vitro study was to determine the antimicrobial capacity of a Berberis vulgaris plant extract on the bacteria being associated with caries including, Streptococcus mutans, S. sobrinus, S. sanguinis, S. salivaris and Lactobacillus rhamnosus. MATERIAL AND METHODS: Chlorhexidine 2% (CHX) mouthwash and ampicillin (10 μg/disk) were applied as positive control groups. Inhibition zone, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) related to stem, leaf and fruit of B. vulgaris plant were recorded for every five bacteria. Data were analyzed using SPSS ver. 22, one‐way ANOVA repeated measure and post hoc Tukey statistical test. The significance level was set at p < 0.05. RESULTS: There were no significant differences between the antimicrobial capacity of the positive controls and the extract from the stem and fruit of B. vulgaris (p > 0.05). The MIC values of the extract from the stem were significantly lower against S. sobrinus (64 μg/ml) and L. rhamnosus (128 μg/ml). The MIC value of the extract against S. mutans was significantly lower in the fruit group (64 μg/mL). The MBC value of the extract against S. sobrinus and L. rhamnosus was significantly lower in the stem group (128 μg/ml). The MBC value against S. mutans was significantly lower in the fruit group (128 μg/ml). CONCLUSIONS: The results showed that CHX and B. vulgaris plant extract have similar antimicrobial activity against bacteria being associated with caries. Therefore, B. vulgaris, which shows antibacterial capacity, could be considered for further investigation as a safe, phytotherapeutic mouthwash to prevent dental caries. John Wiley and Sons Inc. 2020-12-15 /pmc/articles/PMC8404507/ /pubmed/33325156 http://dx.doi.org/10.1002/cre2.379 Text en © 2020 The Authors. Clinical and Experimental Dental Research published by John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Kazemipoor, Maryam Fadaei Tehrani, Pooya Zandi, Hengameh Golvardi Yazdi, Reza Chemical composition and antibacterial activity of Berberis vulgaris (barberry) against bacteria associated with caries |
title | Chemical composition and antibacterial activity of Berberis vulgaris (barberry) against bacteria associated with caries |
title_full | Chemical composition and antibacterial activity of Berberis vulgaris (barberry) against bacteria associated with caries |
title_fullStr | Chemical composition and antibacterial activity of Berberis vulgaris (barberry) against bacteria associated with caries |
title_full_unstemmed | Chemical composition and antibacterial activity of Berberis vulgaris (barberry) against bacteria associated with caries |
title_short | Chemical composition and antibacterial activity of Berberis vulgaris (barberry) against bacteria associated with caries |
title_sort | chemical composition and antibacterial activity of berberis vulgaris (barberry) against bacteria associated with caries |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8404507/ https://www.ncbi.nlm.nih.gov/pubmed/33325156 http://dx.doi.org/10.1002/cre2.379 |
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