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Protective activity of mRNA vaccines against ancestral and variant SARS-CoV-2 strains

Although mRNA vaccines prevent COVID-19, variants jeopardize their efficacy as immunity wanes. Here, we assessed the immunogenicity and protective activity of historical (mRNA-1273, designed for Wuhan-1 spike) or modified (mRNA-1273.351, designed for B.1.351 spike) preclinical Moderna mRNA vaccines...

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Autores principales: Ying, Baoling, Whitener, Bradley, VanBlargan, Laura A., Hassan, Ahmed O., Shrihari, Swathi, Liang, Chieh-Yu, Karl, Courtney E., Mackin, Samantha, Chen, Rita E., Kafai, Natasha M., Wilks, Samuel H., Smith, Derek J., Carreño, Juan Manuel, Singh, Gagandeep, Krammer, Florian, Carfi, Andrea, Elbashir, Sayda, Edwards, Darin K., Thackray, Larissa B., Diamond, Michael S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8404887/
https://www.ncbi.nlm.nih.gov/pubmed/34462745
http://dx.doi.org/10.1101/2021.08.25.457693
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author Ying, Baoling
Whitener, Bradley
VanBlargan, Laura A.
Hassan, Ahmed O.
Shrihari, Swathi
Liang, Chieh-Yu
Karl, Courtney E.
Mackin, Samantha
Chen, Rita E.
Kafai, Natasha M.
Wilks, Samuel H.
Smith, Derek J.
Carreño, Juan Manuel
Singh, Gagandeep
Krammer, Florian
Carfi, Andrea
Elbashir, Sayda
Edwards, Darin K.
Thackray, Larissa B.
Diamond, Michael S.
author_facet Ying, Baoling
Whitener, Bradley
VanBlargan, Laura A.
Hassan, Ahmed O.
Shrihari, Swathi
Liang, Chieh-Yu
Karl, Courtney E.
Mackin, Samantha
Chen, Rita E.
Kafai, Natasha M.
Wilks, Samuel H.
Smith, Derek J.
Carreño, Juan Manuel
Singh, Gagandeep
Krammer, Florian
Carfi, Andrea
Elbashir, Sayda
Edwards, Darin K.
Thackray, Larissa B.
Diamond, Michael S.
author_sort Ying, Baoling
collection PubMed
description Although mRNA vaccines prevent COVID-19, variants jeopardize their efficacy as immunity wanes. Here, we assessed the immunogenicity and protective activity of historical (mRNA-1273, designed for Wuhan-1 spike) or modified (mRNA-1273.351, designed for B.1.351 spike) preclinical Moderna mRNA vaccines in 129S2 and K18-hACE2 mice. Immunization with high or low dose formulations of mRNA vaccines induced neutralizing antibodies in serum against ancestral SARS-CoV-2 and several variants, although levels were lower particularly against the B.1.617.2 (Delta) virus. Protection against weight loss and lung pathology was observed with all high-dose vaccines against all viruses. Nonetheless, low-dose formulations of the vaccines, which produced lower magnitude antibody and T cell responses, and serve as a possible model for waning immunity, showed breakthrough lung infection and pneumonia with B.1.617.2. Thus, as levels of immunity induced by mRNA vaccines decline, breakthrough infection and disease likely will occur with some SARS-CoV-2 variants, suggesting a need for additional booster regimens.
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spelling pubmed-84048872021-08-31 Protective activity of mRNA vaccines against ancestral and variant SARS-CoV-2 strains Ying, Baoling Whitener, Bradley VanBlargan, Laura A. Hassan, Ahmed O. Shrihari, Swathi Liang, Chieh-Yu Karl, Courtney E. Mackin, Samantha Chen, Rita E. Kafai, Natasha M. Wilks, Samuel H. Smith, Derek J. Carreño, Juan Manuel Singh, Gagandeep Krammer, Florian Carfi, Andrea Elbashir, Sayda Edwards, Darin K. Thackray, Larissa B. Diamond, Michael S. bioRxiv Article Although mRNA vaccines prevent COVID-19, variants jeopardize their efficacy as immunity wanes. Here, we assessed the immunogenicity and protective activity of historical (mRNA-1273, designed for Wuhan-1 spike) or modified (mRNA-1273.351, designed for B.1.351 spike) preclinical Moderna mRNA vaccines in 129S2 and K18-hACE2 mice. Immunization with high or low dose formulations of mRNA vaccines induced neutralizing antibodies in serum against ancestral SARS-CoV-2 and several variants, although levels were lower particularly against the B.1.617.2 (Delta) virus. Protection against weight loss and lung pathology was observed with all high-dose vaccines against all viruses. Nonetheless, low-dose formulations of the vaccines, which produced lower magnitude antibody and T cell responses, and serve as a possible model for waning immunity, showed breakthrough lung infection and pneumonia with B.1.617.2. Thus, as levels of immunity induced by mRNA vaccines decline, breakthrough infection and disease likely will occur with some SARS-CoV-2 variants, suggesting a need for additional booster regimens. Cold Spring Harbor Laboratory 2021-08-26 /pmc/articles/PMC8404887/ /pubmed/34462745 http://dx.doi.org/10.1101/2021.08.25.457693 Text en https://creativecommons.org/licenses/by-nc-nd/4.0/This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which allows reusers to copy and distribute the material in any medium or format in unadapted form only, for noncommercial purposes only, and only so long as attribution is given to the creator.
spellingShingle Article
Ying, Baoling
Whitener, Bradley
VanBlargan, Laura A.
Hassan, Ahmed O.
Shrihari, Swathi
Liang, Chieh-Yu
Karl, Courtney E.
Mackin, Samantha
Chen, Rita E.
Kafai, Natasha M.
Wilks, Samuel H.
Smith, Derek J.
Carreño, Juan Manuel
Singh, Gagandeep
Krammer, Florian
Carfi, Andrea
Elbashir, Sayda
Edwards, Darin K.
Thackray, Larissa B.
Diamond, Michael S.
Protective activity of mRNA vaccines against ancestral and variant SARS-CoV-2 strains
title Protective activity of mRNA vaccines against ancestral and variant SARS-CoV-2 strains
title_full Protective activity of mRNA vaccines against ancestral and variant SARS-CoV-2 strains
title_fullStr Protective activity of mRNA vaccines against ancestral and variant SARS-CoV-2 strains
title_full_unstemmed Protective activity of mRNA vaccines against ancestral and variant SARS-CoV-2 strains
title_short Protective activity of mRNA vaccines against ancestral and variant SARS-CoV-2 strains
title_sort protective activity of mrna vaccines against ancestral and variant sars-cov-2 strains
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8404887/
https://www.ncbi.nlm.nih.gov/pubmed/34462745
http://dx.doi.org/10.1101/2021.08.25.457693
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