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Cholecystokinin Expression in the Development of Myocardial Hypertrophy

BACKGROUND: Expression of cholecystokinin is found in myocardial tissues as a gastrointestinal hormone and may be involved in cardiovascular regulation. However, it is unclear whether there is an increase in cholecystokinin expression in myocardial hypertrophy progression induced by abdominal aortic...

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Autores principales: Han, Zhongshu, Bi, Sheng, Xu, Yongsheng, Dong, Xiaoying, Mei, Lixia, Lin, Hailong, Li, Xueqi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8405328/
https://www.ncbi.nlm.nih.gov/pubmed/34497680
http://dx.doi.org/10.1155/2021/8231559
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author Han, Zhongshu
Bi, Sheng
Xu, Yongsheng
Dong, Xiaoying
Mei, Lixia
Lin, Hailong
Li, Xueqi
author_facet Han, Zhongshu
Bi, Sheng
Xu, Yongsheng
Dong, Xiaoying
Mei, Lixia
Lin, Hailong
Li, Xueqi
author_sort Han, Zhongshu
collection PubMed
description BACKGROUND: Expression of cholecystokinin is found in myocardial tissues as a gastrointestinal hormone and may be involved in cardiovascular regulation. However, it is unclear whether there is an increase in cholecystokinin expression in myocardial hypertrophy progression induced by abdominal aortic constriction. The study is aimed at exploring the relationship between cholecystokinin expression and myocardial hypertrophy. METHODS: We randomly divided the 70 Sprague-Dawley rats into two groups: the sham operation group and the abdominal aortic constriction group. The hearts of rats were measured by echocardiography, and myocardial tissues and blood were collected at 4 weeks, 8 weeks, and 12 weeks after surgery. Morphological changes were assessed by microscopy. The cholecystokinin expression was evaluated by immunochemistry, Western blotting, quantitative real-time polymerase chain reaction, and enzyme-linked immunosorbent assay. RESULTS: The relative protein levels of cholecystokinin were significantly increased in the abdominal aortic constriction groups compared with the corresponding sham operation groups at 8 weeks and 12 weeks. The cholecystokinin mRNA in the abdominal aortic constriction groups was significantly higher than the time-matched sham operation groups. Changes in the left ventricular wall thickness were positively correlated with the relative protein levels of cholecystokinin and the mRNA of cholecystokinin. CONCLUSIONS: The development of myocardial hypertrophy can affect the cholecystokinin expression of myocardial tissues.
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spelling pubmed-84053282021-09-07 Cholecystokinin Expression in the Development of Myocardial Hypertrophy Han, Zhongshu Bi, Sheng Xu, Yongsheng Dong, Xiaoying Mei, Lixia Lin, Hailong Li, Xueqi Scanning Research Article BACKGROUND: Expression of cholecystokinin is found in myocardial tissues as a gastrointestinal hormone and may be involved in cardiovascular regulation. However, it is unclear whether there is an increase in cholecystokinin expression in myocardial hypertrophy progression induced by abdominal aortic constriction. The study is aimed at exploring the relationship between cholecystokinin expression and myocardial hypertrophy. METHODS: We randomly divided the 70 Sprague-Dawley rats into two groups: the sham operation group and the abdominal aortic constriction group. The hearts of rats were measured by echocardiography, and myocardial tissues and blood were collected at 4 weeks, 8 weeks, and 12 weeks after surgery. Morphological changes were assessed by microscopy. The cholecystokinin expression was evaluated by immunochemistry, Western blotting, quantitative real-time polymerase chain reaction, and enzyme-linked immunosorbent assay. RESULTS: The relative protein levels of cholecystokinin were significantly increased in the abdominal aortic constriction groups compared with the corresponding sham operation groups at 8 weeks and 12 weeks. The cholecystokinin mRNA in the abdominal aortic constriction groups was significantly higher than the time-matched sham operation groups. Changes in the left ventricular wall thickness were positively correlated with the relative protein levels of cholecystokinin and the mRNA of cholecystokinin. CONCLUSIONS: The development of myocardial hypertrophy can affect the cholecystokinin expression of myocardial tissues. Hindawi 2021-08-21 /pmc/articles/PMC8405328/ /pubmed/34497680 http://dx.doi.org/10.1155/2021/8231559 Text en Copyright © 2021 Zhongshu Han et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Han, Zhongshu
Bi, Sheng
Xu, Yongsheng
Dong, Xiaoying
Mei, Lixia
Lin, Hailong
Li, Xueqi
Cholecystokinin Expression in the Development of Myocardial Hypertrophy
title Cholecystokinin Expression in the Development of Myocardial Hypertrophy
title_full Cholecystokinin Expression in the Development of Myocardial Hypertrophy
title_fullStr Cholecystokinin Expression in the Development of Myocardial Hypertrophy
title_full_unstemmed Cholecystokinin Expression in the Development of Myocardial Hypertrophy
title_short Cholecystokinin Expression in the Development of Myocardial Hypertrophy
title_sort cholecystokinin expression in the development of myocardial hypertrophy
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8405328/
https://www.ncbi.nlm.nih.gov/pubmed/34497680
http://dx.doi.org/10.1155/2021/8231559
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