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Potential toxicity of leachate from the municipal landfill in view of the possibility of their migration to the environment through infiltration into groundwater

Leachate from landfills is a product of complex biological and physicochemical processes occurring during waste storage. In the present study, the toxicity of landfill leachate (LL) to human and bacterial cells was investigated for better understanding of LL environmental toxicity. Studies regarding...

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Autores principales: Jabłońska-Trypuć, Agata, Wydro, Urszula, Wołejko, Elżbieta, Pietryczuk, Anna, Cudowski, Adam, Leszczyński, Jacek, Rodziewicz, Joanna, Janczukowicz, Wojciech, Butarewicz, Andrzej
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8405471/
https://www.ncbi.nlm.nih.gov/pubmed/33675453
http://dx.doi.org/10.1007/s10653-021-00867-5
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author Jabłońska-Trypuć, Agata
Wydro, Urszula
Wołejko, Elżbieta
Pietryczuk, Anna
Cudowski, Adam
Leszczyński, Jacek
Rodziewicz, Joanna
Janczukowicz, Wojciech
Butarewicz, Andrzej
author_facet Jabłońska-Trypuć, Agata
Wydro, Urszula
Wołejko, Elżbieta
Pietryczuk, Anna
Cudowski, Adam
Leszczyński, Jacek
Rodziewicz, Joanna
Janczukowicz, Wojciech
Butarewicz, Andrzej
author_sort Jabłońska-Trypuć, Agata
collection PubMed
description Leachate from landfills is a product of complex biological and physicochemical processes occurring during waste storage. In the present study, the toxicity of landfill leachate (LL) to human and bacterial cells was investigated for better understanding of LL environmental toxicity. Studies regarding LL physicochemical properties and cytotoxicity analysis were conducted. In Escherichia coli, Pseudomonas fluorescens, Bacillus subtilis, fibroblasts and melanoma A-375 cells, cell viability assays were applied. For the determination of LL antibacterial activity, twofold dilution series of LL were prepared in the range from 50% to 0.1% (50%, 25%, 12.5%, 6.25%, 3.13%, 1.56%, 0.78%, 0.39%, 0.2%, 0.1%). Human cells viability was examined at LL concentrations of 0.1%, 0.5%, 1%, 1.5%, 2%, 2.5%, 5%, 10%, 15%, 20% and 30%. ROS (reactive oxygen species) content and apoptosis level were also measured in bacterial and human cells under the influence of LL. Unexpectedly obtained results indicate stimulation of bacterial viability by LL. Fibroblasts under the influence of LL showed decrease in their viability and increase in apoptosis level and A-375 melanoma cells showed an increase in relative viability and decrease in apoptosis. ROS level in bacterial cells was elevated in higher LL concentrations and decreased in lower LL concentrations. In human cells, ROS content was rather high in both tested cell lines. Presented results indicate cytotoxic potential of analyzed LL and the necessity of LL monitoring because it may pose a health hazard for exposed human populations and the whole human environment.
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spelling pubmed-84054712021-09-09 Potential toxicity of leachate from the municipal landfill in view of the possibility of their migration to the environment through infiltration into groundwater Jabłońska-Trypuć, Agata Wydro, Urszula Wołejko, Elżbieta Pietryczuk, Anna Cudowski, Adam Leszczyński, Jacek Rodziewicz, Joanna Janczukowicz, Wojciech Butarewicz, Andrzej Environ Geochem Health Original Paper Leachate from landfills is a product of complex biological and physicochemical processes occurring during waste storage. In the present study, the toxicity of landfill leachate (LL) to human and bacterial cells was investigated for better understanding of LL environmental toxicity. Studies regarding LL physicochemical properties and cytotoxicity analysis were conducted. In Escherichia coli, Pseudomonas fluorescens, Bacillus subtilis, fibroblasts and melanoma A-375 cells, cell viability assays were applied. For the determination of LL antibacterial activity, twofold dilution series of LL were prepared in the range from 50% to 0.1% (50%, 25%, 12.5%, 6.25%, 3.13%, 1.56%, 0.78%, 0.39%, 0.2%, 0.1%). Human cells viability was examined at LL concentrations of 0.1%, 0.5%, 1%, 1.5%, 2%, 2.5%, 5%, 10%, 15%, 20% and 30%. ROS (reactive oxygen species) content and apoptosis level were also measured in bacterial and human cells under the influence of LL. Unexpectedly obtained results indicate stimulation of bacterial viability by LL. Fibroblasts under the influence of LL showed decrease in their viability and increase in apoptosis level and A-375 melanoma cells showed an increase in relative viability and decrease in apoptosis. ROS level in bacterial cells was elevated in higher LL concentrations and decreased in lower LL concentrations. In human cells, ROS content was rather high in both tested cell lines. Presented results indicate cytotoxic potential of analyzed LL and the necessity of LL monitoring because it may pose a health hazard for exposed human populations and the whole human environment. Springer Netherlands 2021-03-06 2021 /pmc/articles/PMC8405471/ /pubmed/33675453 http://dx.doi.org/10.1007/s10653-021-00867-5 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Original Paper
Jabłońska-Trypuć, Agata
Wydro, Urszula
Wołejko, Elżbieta
Pietryczuk, Anna
Cudowski, Adam
Leszczyński, Jacek
Rodziewicz, Joanna
Janczukowicz, Wojciech
Butarewicz, Andrzej
Potential toxicity of leachate from the municipal landfill in view of the possibility of their migration to the environment through infiltration into groundwater
title Potential toxicity of leachate from the municipal landfill in view of the possibility of their migration to the environment through infiltration into groundwater
title_full Potential toxicity of leachate from the municipal landfill in view of the possibility of their migration to the environment through infiltration into groundwater
title_fullStr Potential toxicity of leachate from the municipal landfill in view of the possibility of their migration to the environment through infiltration into groundwater
title_full_unstemmed Potential toxicity of leachate from the municipal landfill in view of the possibility of their migration to the environment through infiltration into groundwater
title_short Potential toxicity of leachate from the municipal landfill in view of the possibility of their migration to the environment through infiltration into groundwater
title_sort potential toxicity of leachate from the municipal landfill in view of the possibility of their migration to the environment through infiltration into groundwater
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8405471/
https://www.ncbi.nlm.nih.gov/pubmed/33675453
http://dx.doi.org/10.1007/s10653-021-00867-5
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