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Microbial Diversity and Function in Shallow Subsurface Sediment and Oceanic Lithosphere of the Atlantis Massif
The marine lithospheric subsurface is one of the largest biospheres on Earth; however, little is known about the identity and ecological function of microorganisms found in low abundance in this habitat, though these organisms impact global-scale biogeochemical cycling. Here, we describe the diversi...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society for Microbiology
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8406227/ https://www.ncbi.nlm.nih.gov/pubmed/34340550 http://dx.doi.org/10.1128/mBio.00490-21 |
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author | Goordial, J. D’Angelo, T. Labonté, J. M. Poulton, N. J. Brown, J. M. Stepanauskas, R. Früh-Green, G. L. Orcutt, B. N. |
author_facet | Goordial, J. D’Angelo, T. Labonté, J. M. Poulton, N. J. Brown, J. M. Stepanauskas, R. Früh-Green, G. L. Orcutt, B. N. |
author_sort | Goordial, J. |
collection | PubMed |
description | The marine lithospheric subsurface is one of the largest biospheres on Earth; however, little is known about the identity and ecological function of microorganisms found in low abundance in this habitat, though these organisms impact global-scale biogeochemical cycling. Here, we describe the diversity and metabolic potential of sediment and endolithic (within rock) microbial communities found in ultrasmall amounts (10(1) to 10(4) cells cm(−3)) in the subsurface of the Atlantis Massif, an oceanic core complex on the Mid-Atlantic Ridge that was sampled on International Ocean Discovery Program (IODP) Expedition 357. This study used fluorescence-activated cell sorting (FACS) to enable the first amplicon, metagenomic, and single-cell genomic study of the shallow (<20 m below seafloor) subsurface of an actively serpentinizing marine system. The shallow subsurface biosphere of the Atlantis Massif was found to be distinct from communities observed in the nearby Lost City alkaline hydrothermal fluids and chimneys, yet similar to other low-temperature, aerobic subsurface settings. Genes associated with autotrophy were rare, although heterotrophy and aerobic carbon monoxide and formate cycling metabolisms were identified. Overall, this study reveals that the shallow subsurface of an oceanic core complex hosts a biosphere that is not fueled by active serpentinization reactions and by-products. |
format | Online Article Text |
id | pubmed-8406227 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | American Society for Microbiology |
record_format | MEDLINE/PubMed |
spelling | pubmed-84062272021-09-09 Microbial Diversity and Function in Shallow Subsurface Sediment and Oceanic Lithosphere of the Atlantis Massif Goordial, J. D’Angelo, T. Labonté, J. M. Poulton, N. J. Brown, J. M. Stepanauskas, R. Früh-Green, G. L. Orcutt, B. N. mBio Research Article The marine lithospheric subsurface is one of the largest biospheres on Earth; however, little is known about the identity and ecological function of microorganisms found in low abundance in this habitat, though these organisms impact global-scale biogeochemical cycling. Here, we describe the diversity and metabolic potential of sediment and endolithic (within rock) microbial communities found in ultrasmall amounts (10(1) to 10(4) cells cm(−3)) in the subsurface of the Atlantis Massif, an oceanic core complex on the Mid-Atlantic Ridge that was sampled on International Ocean Discovery Program (IODP) Expedition 357. This study used fluorescence-activated cell sorting (FACS) to enable the first amplicon, metagenomic, and single-cell genomic study of the shallow (<20 m below seafloor) subsurface of an actively serpentinizing marine system. The shallow subsurface biosphere of the Atlantis Massif was found to be distinct from communities observed in the nearby Lost City alkaline hydrothermal fluids and chimneys, yet similar to other low-temperature, aerobic subsurface settings. Genes associated with autotrophy were rare, although heterotrophy and aerobic carbon monoxide and formate cycling metabolisms were identified. Overall, this study reveals that the shallow subsurface of an oceanic core complex hosts a biosphere that is not fueled by active serpentinization reactions and by-products. American Society for Microbiology 2021-08-03 /pmc/articles/PMC8406227/ /pubmed/34340550 http://dx.doi.org/10.1128/mBio.00490-21 Text en Copyright © 2021 Goordial et al. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Research Article Goordial, J. D’Angelo, T. Labonté, J. M. Poulton, N. J. Brown, J. M. Stepanauskas, R. Früh-Green, G. L. Orcutt, B. N. Microbial Diversity and Function in Shallow Subsurface Sediment and Oceanic Lithosphere of the Atlantis Massif |
title | Microbial Diversity and Function in Shallow Subsurface Sediment and Oceanic Lithosphere of the Atlantis Massif |
title_full | Microbial Diversity and Function in Shallow Subsurface Sediment and Oceanic Lithosphere of the Atlantis Massif |
title_fullStr | Microbial Diversity and Function in Shallow Subsurface Sediment and Oceanic Lithosphere of the Atlantis Massif |
title_full_unstemmed | Microbial Diversity and Function in Shallow Subsurface Sediment and Oceanic Lithosphere of the Atlantis Massif |
title_short | Microbial Diversity and Function in Shallow Subsurface Sediment and Oceanic Lithosphere of the Atlantis Massif |
title_sort | microbial diversity and function in shallow subsurface sediment and oceanic lithosphere of the atlantis massif |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8406227/ https://www.ncbi.nlm.nih.gov/pubmed/34340550 http://dx.doi.org/10.1128/mBio.00490-21 |
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