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O-Specific Antigen-Dependent Surface Hydrophobicity Mediates Aggregate Assembly Type in Pseudomonas aeruginosa

Bacteria live in spatially organized aggregates during chronic infections, where they adapt to the host environment, evade immune responses, and resist therapeutic interventions. Although it is known that environmental factors such as polymers influence bacterial aggregation, it is not clear how bac...

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Detalles Bibliográficos
Autores principales: Azimi, Sheyda, Thomas, Jacob, Cleland, Sara E., Curtis, Jennifer E., Goldberg, Joanna B., Diggle, Stephen P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8406328/
https://www.ncbi.nlm.nih.gov/pubmed/34372703
http://dx.doi.org/10.1128/mBio.00860-21
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author Azimi, Sheyda
Thomas, Jacob
Cleland, Sara E.
Curtis, Jennifer E.
Goldberg, Joanna B.
Diggle, Stephen P.
author_facet Azimi, Sheyda
Thomas, Jacob
Cleland, Sara E.
Curtis, Jennifer E.
Goldberg, Joanna B.
Diggle, Stephen P.
author_sort Azimi, Sheyda
collection PubMed
description Bacteria live in spatially organized aggregates during chronic infections, where they adapt to the host environment, evade immune responses, and resist therapeutic interventions. Although it is known that environmental factors such as polymers influence bacterial aggregation, it is not clear how bacterial adaptation during chronic infection impacts the formation and spatial organization of aggregates in the presence of polymers. Here, we show that in an in vitro model of cystic fibrosis (CF) containing the polymers extracellular DNA (eDNA) and mucin, O-specific antigen is a major factor determining the formation of two distinct aggregate assembly types of Pseudomonas aeruginosa due to alterations in cell surface hydrophobicity. Our findings suggest that during chronic infection, the interplay between cell surface properties and polymers in the environment may influence the formation and structure of bacterial aggregates, which would shed new light on the fitness costs and benefits of O-antigen production in environments such as CF lungs.
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spelling pubmed-84063282021-09-09 O-Specific Antigen-Dependent Surface Hydrophobicity Mediates Aggregate Assembly Type in Pseudomonas aeruginosa Azimi, Sheyda Thomas, Jacob Cleland, Sara E. Curtis, Jennifer E. Goldberg, Joanna B. Diggle, Stephen P. mBio Research Article Bacteria live in spatially organized aggregates during chronic infections, where they adapt to the host environment, evade immune responses, and resist therapeutic interventions. Although it is known that environmental factors such as polymers influence bacterial aggregation, it is not clear how bacterial adaptation during chronic infection impacts the formation and spatial organization of aggregates in the presence of polymers. Here, we show that in an in vitro model of cystic fibrosis (CF) containing the polymers extracellular DNA (eDNA) and mucin, O-specific antigen is a major factor determining the formation of two distinct aggregate assembly types of Pseudomonas aeruginosa due to alterations in cell surface hydrophobicity. Our findings suggest that during chronic infection, the interplay between cell surface properties and polymers in the environment may influence the formation and structure of bacterial aggregates, which would shed new light on the fitness costs and benefits of O-antigen production in environments such as CF lungs. American Society for Microbiology 2021-08-10 /pmc/articles/PMC8406328/ /pubmed/34372703 http://dx.doi.org/10.1128/mBio.00860-21 Text en Copyright © 2021 Azimi et al. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Article
Azimi, Sheyda
Thomas, Jacob
Cleland, Sara E.
Curtis, Jennifer E.
Goldberg, Joanna B.
Diggle, Stephen P.
O-Specific Antigen-Dependent Surface Hydrophobicity Mediates Aggregate Assembly Type in Pseudomonas aeruginosa
title O-Specific Antigen-Dependent Surface Hydrophobicity Mediates Aggregate Assembly Type in Pseudomonas aeruginosa
title_full O-Specific Antigen-Dependent Surface Hydrophobicity Mediates Aggregate Assembly Type in Pseudomonas aeruginosa
title_fullStr O-Specific Antigen-Dependent Surface Hydrophobicity Mediates Aggregate Assembly Type in Pseudomonas aeruginosa
title_full_unstemmed O-Specific Antigen-Dependent Surface Hydrophobicity Mediates Aggregate Assembly Type in Pseudomonas aeruginosa
title_short O-Specific Antigen-Dependent Surface Hydrophobicity Mediates Aggregate Assembly Type in Pseudomonas aeruginosa
title_sort o-specific antigen-dependent surface hydrophobicity mediates aggregate assembly type in pseudomonas aeruginosa
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8406328/
https://www.ncbi.nlm.nih.gov/pubmed/34372703
http://dx.doi.org/10.1128/mBio.00860-21
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