Cargando…

Genomic analysis of Neisseria elongata isolate from a patient with infective endocarditis

Neisseria elongata is part of the commensal microbiota of the oropharynx. Although it is not considered pathogenic to humans, N. elongata has been implicated in several cases of infective endocarditis (IE). Here, we report a case of IE caused by N. elongata subsp. nitroreducens (Nel_M001) and compar...

Descripción completa

Detalles Bibliográficos
Autores principales: Schörner, Marcos André, Passarelli‐Araujo, Hemanoel, Scheffer, Mara Cristina, Hartmann Barazzetti, Fernando, Motta Martins, Jessica, de Melo Machado, Hanalydia, Palmeiro, Jussara Kasuko, Bazzo, Maria Luiza
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8406478/
https://www.ncbi.nlm.nih.gov/pubmed/34038628
http://dx.doi.org/10.1002/2211-5463.13201
_version_ 1783746516626702336
author Schörner, Marcos André
Passarelli‐Araujo, Hemanoel
Scheffer, Mara Cristina
Hartmann Barazzetti, Fernando
Motta Martins, Jessica
de Melo Machado, Hanalydia
Palmeiro, Jussara Kasuko
Bazzo, Maria Luiza
author_facet Schörner, Marcos André
Passarelli‐Araujo, Hemanoel
Scheffer, Mara Cristina
Hartmann Barazzetti, Fernando
Motta Martins, Jessica
de Melo Machado, Hanalydia
Palmeiro, Jussara Kasuko
Bazzo, Maria Luiza
author_sort Schörner, Marcos André
collection PubMed
description Neisseria elongata is part of the commensal microbiota of the oropharynx. Although it is not considered pathogenic to humans, N. elongata has been implicated in several cases of infective endocarditis (IE). Here, we report a case of IE caused by N. elongata subsp. nitroreducens (Nel_M001) and compare its genome with 17 N. elongata genomes available in GenBank. We also evaluated resistance and virulence profiles with Comprehensive Antibiotic Resistance and Virulence Finder databases. The results showed a wide diversity among N. elongata isolates. Based on the pangenome cumulative curve, we demonstrate that N. elongata has an open pangenome. We found several different resistance genes, mainly associated with antibiotic efflux pumps. A wide range of virulence genes was observed, predominantly pilus formation genes. Nel_M001 was the only isolate to present two copies of some pilus genes and not present nspA gene. Together, our results provide insights into how this commensal microorganism can cause IE and may assist further biological investigations on nonpathogenic Neisseria spp. Case reporting and pangenome analyses are critical for enhancing our understanding of IE pathogenesis, as well as for alerting physicians and microbiologists to enable rapid identification and treatment to avoid unfavorable outcomes.
format Online
Article
Text
id pubmed-8406478
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher John Wiley and Sons Inc.
record_format MEDLINE/PubMed
spelling pubmed-84064782021-09-03 Genomic analysis of Neisseria elongata isolate from a patient with infective endocarditis Schörner, Marcos André Passarelli‐Araujo, Hemanoel Scheffer, Mara Cristina Hartmann Barazzetti, Fernando Motta Martins, Jessica de Melo Machado, Hanalydia Palmeiro, Jussara Kasuko Bazzo, Maria Luiza FEBS Open Bio Research Articles Neisseria elongata is part of the commensal microbiota of the oropharynx. Although it is not considered pathogenic to humans, N. elongata has been implicated in several cases of infective endocarditis (IE). Here, we report a case of IE caused by N. elongata subsp. nitroreducens (Nel_M001) and compare its genome with 17 N. elongata genomes available in GenBank. We also evaluated resistance and virulence profiles with Comprehensive Antibiotic Resistance and Virulence Finder databases. The results showed a wide diversity among N. elongata isolates. Based on the pangenome cumulative curve, we demonstrate that N. elongata has an open pangenome. We found several different resistance genes, mainly associated with antibiotic efflux pumps. A wide range of virulence genes was observed, predominantly pilus formation genes. Nel_M001 was the only isolate to present two copies of some pilus genes and not present nspA gene. Together, our results provide insights into how this commensal microorganism can cause IE and may assist further biological investigations on nonpathogenic Neisseria spp. Case reporting and pangenome analyses are critical for enhancing our understanding of IE pathogenesis, as well as for alerting physicians and microbiologists to enable rapid identification and treatment to avoid unfavorable outcomes. John Wiley and Sons Inc. 2021-06-15 /pmc/articles/PMC8406478/ /pubmed/34038628 http://dx.doi.org/10.1002/2211-5463.13201 Text en © 2021 The Authors. FEBS Open Bio published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Schörner, Marcos André
Passarelli‐Araujo, Hemanoel
Scheffer, Mara Cristina
Hartmann Barazzetti, Fernando
Motta Martins, Jessica
de Melo Machado, Hanalydia
Palmeiro, Jussara Kasuko
Bazzo, Maria Luiza
Genomic analysis of Neisseria elongata isolate from a patient with infective endocarditis
title Genomic analysis of Neisseria elongata isolate from a patient with infective endocarditis
title_full Genomic analysis of Neisseria elongata isolate from a patient with infective endocarditis
title_fullStr Genomic analysis of Neisseria elongata isolate from a patient with infective endocarditis
title_full_unstemmed Genomic analysis of Neisseria elongata isolate from a patient with infective endocarditis
title_short Genomic analysis of Neisseria elongata isolate from a patient with infective endocarditis
title_sort genomic analysis of neisseria elongata isolate from a patient with infective endocarditis
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8406478/
https://www.ncbi.nlm.nih.gov/pubmed/34038628
http://dx.doi.org/10.1002/2211-5463.13201
work_keys_str_mv AT schornermarcosandre genomicanalysisofneisseriaelongataisolatefromapatientwithinfectiveendocarditis
AT passarelliaraujohemanoel genomicanalysisofneisseriaelongataisolatefromapatientwithinfectiveendocarditis
AT scheffermaracristina genomicanalysisofneisseriaelongataisolatefromapatientwithinfectiveendocarditis
AT hartmannbarazzettifernando genomicanalysisofneisseriaelongataisolatefromapatientwithinfectiveendocarditis
AT mottamartinsjessica genomicanalysisofneisseriaelongataisolatefromapatientwithinfectiveendocarditis
AT demelomachadohanalydia genomicanalysisofneisseriaelongataisolatefromapatientwithinfectiveendocarditis
AT palmeirojussarakasuko genomicanalysisofneisseriaelongataisolatefromapatientwithinfectiveendocarditis
AT bazzomarialuiza genomicanalysisofneisseriaelongataisolatefromapatientwithinfectiveendocarditis