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Genomic analysis of Neisseria elongata isolate from a patient with infective endocarditis
Neisseria elongata is part of the commensal microbiota of the oropharynx. Although it is not considered pathogenic to humans, N. elongata has been implicated in several cases of infective endocarditis (IE). Here, we report a case of IE caused by N. elongata subsp. nitroreducens (Nel_M001) and compar...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8406478/ https://www.ncbi.nlm.nih.gov/pubmed/34038628 http://dx.doi.org/10.1002/2211-5463.13201 |
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author | Schörner, Marcos André Passarelli‐Araujo, Hemanoel Scheffer, Mara Cristina Hartmann Barazzetti, Fernando Motta Martins, Jessica de Melo Machado, Hanalydia Palmeiro, Jussara Kasuko Bazzo, Maria Luiza |
author_facet | Schörner, Marcos André Passarelli‐Araujo, Hemanoel Scheffer, Mara Cristina Hartmann Barazzetti, Fernando Motta Martins, Jessica de Melo Machado, Hanalydia Palmeiro, Jussara Kasuko Bazzo, Maria Luiza |
author_sort | Schörner, Marcos André |
collection | PubMed |
description | Neisseria elongata is part of the commensal microbiota of the oropharynx. Although it is not considered pathogenic to humans, N. elongata has been implicated in several cases of infective endocarditis (IE). Here, we report a case of IE caused by N. elongata subsp. nitroreducens (Nel_M001) and compare its genome with 17 N. elongata genomes available in GenBank. We also evaluated resistance and virulence profiles with Comprehensive Antibiotic Resistance and Virulence Finder databases. The results showed a wide diversity among N. elongata isolates. Based on the pangenome cumulative curve, we demonstrate that N. elongata has an open pangenome. We found several different resistance genes, mainly associated with antibiotic efflux pumps. A wide range of virulence genes was observed, predominantly pilus formation genes. Nel_M001 was the only isolate to present two copies of some pilus genes and not present nspA gene. Together, our results provide insights into how this commensal microorganism can cause IE and may assist further biological investigations on nonpathogenic Neisseria spp. Case reporting and pangenome analyses are critical for enhancing our understanding of IE pathogenesis, as well as for alerting physicians and microbiologists to enable rapid identification and treatment to avoid unfavorable outcomes. |
format | Online Article Text |
id | pubmed-8406478 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-84064782021-09-03 Genomic analysis of Neisseria elongata isolate from a patient with infective endocarditis Schörner, Marcos André Passarelli‐Araujo, Hemanoel Scheffer, Mara Cristina Hartmann Barazzetti, Fernando Motta Martins, Jessica de Melo Machado, Hanalydia Palmeiro, Jussara Kasuko Bazzo, Maria Luiza FEBS Open Bio Research Articles Neisseria elongata is part of the commensal microbiota of the oropharynx. Although it is not considered pathogenic to humans, N. elongata has been implicated in several cases of infective endocarditis (IE). Here, we report a case of IE caused by N. elongata subsp. nitroreducens (Nel_M001) and compare its genome with 17 N. elongata genomes available in GenBank. We also evaluated resistance and virulence profiles with Comprehensive Antibiotic Resistance and Virulence Finder databases. The results showed a wide diversity among N. elongata isolates. Based on the pangenome cumulative curve, we demonstrate that N. elongata has an open pangenome. We found several different resistance genes, mainly associated with antibiotic efflux pumps. A wide range of virulence genes was observed, predominantly pilus formation genes. Nel_M001 was the only isolate to present two copies of some pilus genes and not present nspA gene. Together, our results provide insights into how this commensal microorganism can cause IE and may assist further biological investigations on nonpathogenic Neisseria spp. Case reporting and pangenome analyses are critical for enhancing our understanding of IE pathogenesis, as well as for alerting physicians and microbiologists to enable rapid identification and treatment to avoid unfavorable outcomes. John Wiley and Sons Inc. 2021-06-15 /pmc/articles/PMC8406478/ /pubmed/34038628 http://dx.doi.org/10.1002/2211-5463.13201 Text en © 2021 The Authors. FEBS Open Bio published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Articles Schörner, Marcos André Passarelli‐Araujo, Hemanoel Scheffer, Mara Cristina Hartmann Barazzetti, Fernando Motta Martins, Jessica de Melo Machado, Hanalydia Palmeiro, Jussara Kasuko Bazzo, Maria Luiza Genomic analysis of Neisseria elongata isolate from a patient with infective endocarditis |
title | Genomic analysis of Neisseria elongata isolate from a patient with infective endocarditis |
title_full | Genomic analysis of Neisseria elongata isolate from a patient with infective endocarditis |
title_fullStr | Genomic analysis of Neisseria elongata isolate from a patient with infective endocarditis |
title_full_unstemmed | Genomic analysis of Neisseria elongata isolate from a patient with infective endocarditis |
title_short | Genomic analysis of Neisseria elongata isolate from a patient with infective endocarditis |
title_sort | genomic analysis of neisseria elongata isolate from a patient with infective endocarditis |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8406478/ https://www.ncbi.nlm.nih.gov/pubmed/34038628 http://dx.doi.org/10.1002/2211-5463.13201 |
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