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Establishment and characterization of an immortalized epicardial cell line
Recently, the increasing significance of the epicardium in cardiac development and regeneration is beginning to be recognized. However, because of the small proportion of primary epicardial cells and the limited cell culture time, further research on the mechanism of epicardial cells is hindered. He...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8406488/ https://www.ncbi.nlm.nih.gov/pubmed/33822475 http://dx.doi.org/10.1111/jcmm.16496 |
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author | Jiang, Haobin Song, Shen Li, Jiacheng Yin, Qianqian Hu, Shengshou Nie, Yu |
author_facet | Jiang, Haobin Song, Shen Li, Jiacheng Yin, Qianqian Hu, Shengshou Nie, Yu |
author_sort | Jiang, Haobin |
collection | PubMed |
description | Recently, the increasing significance of the epicardium in cardiac development and regeneration is beginning to be recognized. However, because of the small proportion of primary epicardial cells and the limited cell culture time, further research on the mechanism of epicardial cells is hindered. Here, we transfected simian virus 40 Large T (SV40‐LT) into primary epicardial cells to establish an immortalized cell line, named EpiSV40. We further demonstrated that EpiSV40 can be easy to culture and has the proliferation, migration and differentiation capacities comparable to primary epicardial cells. EpiSV40 can serve as an ideal in vitro model for epicardial cell research, which will booster the study of the epicardium in cardiac development and heart regeneration. |
format | Online Article Text |
id | pubmed-8406488 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-84064882021-09-03 Establishment and characterization of an immortalized epicardial cell line Jiang, Haobin Song, Shen Li, Jiacheng Yin, Qianqian Hu, Shengshou Nie, Yu J Cell Mol Med Original Articles Recently, the increasing significance of the epicardium in cardiac development and regeneration is beginning to be recognized. However, because of the small proportion of primary epicardial cells and the limited cell culture time, further research on the mechanism of epicardial cells is hindered. Here, we transfected simian virus 40 Large T (SV40‐LT) into primary epicardial cells to establish an immortalized cell line, named EpiSV40. We further demonstrated that EpiSV40 can be easy to culture and has the proliferation, migration and differentiation capacities comparable to primary epicardial cells. EpiSV40 can serve as an ideal in vitro model for epicardial cell research, which will booster the study of the epicardium in cardiac development and heart regeneration. John Wiley and Sons Inc. 2021-04-06 2021-07 /pmc/articles/PMC8406488/ /pubmed/33822475 http://dx.doi.org/10.1111/jcmm.16496 Text en © 2021 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Jiang, Haobin Song, Shen Li, Jiacheng Yin, Qianqian Hu, Shengshou Nie, Yu Establishment and characterization of an immortalized epicardial cell line |
title | Establishment and characterization of an immortalized epicardial cell line |
title_full | Establishment and characterization of an immortalized epicardial cell line |
title_fullStr | Establishment and characterization of an immortalized epicardial cell line |
title_full_unstemmed | Establishment and characterization of an immortalized epicardial cell line |
title_short | Establishment and characterization of an immortalized epicardial cell line |
title_sort | establishment and characterization of an immortalized epicardial cell line |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8406488/ https://www.ncbi.nlm.nih.gov/pubmed/33822475 http://dx.doi.org/10.1111/jcmm.16496 |
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