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Long noncoding RNA SNHG1 promotes TERT expression by sponging miR-18b-5p in breast cancer

BACKGROUND: Long noncoding RNA (lncRNA) small nucleolar RNA host gene 1 (SNHG1) plays a positive role in the progression of human malignant tumors. However, the molecular mechanism of SNHG1 remains elusive in breast cancer. RESULTS: LncRNA SNHG1 was upregulated and had a positive relationship with p...

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Autores principales: Kang, Yujuan, Wan, Lin, Wang, Qin, Yin, Yanling, Liu, Jiena, Liu, Lei, Wu, Hao, Zhang, Lei, Zhang, Xin, Xu, Shouping, Pang, Da
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8407068/
https://www.ncbi.nlm.nih.gov/pubmed/34465388
http://dx.doi.org/10.1186/s13578-021-00675-5
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author Kang, Yujuan
Wan, Lin
Wang, Qin
Yin, Yanling
Liu, Jiena
Liu, Lei
Wu, Hao
Zhang, Lei
Zhang, Xin
Xu, Shouping
Pang, Da
author_facet Kang, Yujuan
Wan, Lin
Wang, Qin
Yin, Yanling
Liu, Jiena
Liu, Lei
Wu, Hao
Zhang, Lei
Zhang, Xin
Xu, Shouping
Pang, Da
author_sort Kang, Yujuan
collection PubMed
description BACKGROUND: Long noncoding RNA (lncRNA) small nucleolar RNA host gene 1 (SNHG1) plays a positive role in the progression of human malignant tumors. However, the molecular mechanism of SNHG1 remains elusive in breast cancer. RESULTS: LncRNA SNHG1 was upregulated and had a positive relationship with poor prognosis according to bioinformatics analysis in pan-cancer including breast cancer. Silencing SNHG1 inhibited tumorigenesis in breast cancer both in vitro and in vivo. Mechanistically, SNHG1 functioned as a competing endogenous RNA (ceRNA) to promote TERT expression by sponging miR-18b-5p in breast cancer. miR-18b-5p acted as a tumor repressor in breast cancer. Moreover, the combination of SNHG1 knockdown and TERT inhibitor administration showed a synergistic inhibitory effect on breast cancer growth in vivo. Finally, E2F1 as a transcription factor, binding to SNHG1 promoter and enhanced SNHG1 transcription in breast cancer. CONCLUSIONS: Our results provide a comprehensive understanding of the oncogenic mechanism of lncRNA SNHG1 in breast cancer. Importantly, we identified a novel E2F1–SNHG1–miR-18b-5p–TERT axis, which may be a potential therapeutic target for breast cancer. Our results also provided a potential treatment for breast cancer when knockdown SNHG1 and TERT inhibitor administration simultaneously. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13578-021-00675-5.
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spelling pubmed-84070682021-09-01 Long noncoding RNA SNHG1 promotes TERT expression by sponging miR-18b-5p in breast cancer Kang, Yujuan Wan, Lin Wang, Qin Yin, Yanling Liu, Jiena Liu, Lei Wu, Hao Zhang, Lei Zhang, Xin Xu, Shouping Pang, Da Cell Biosci Research BACKGROUND: Long noncoding RNA (lncRNA) small nucleolar RNA host gene 1 (SNHG1) plays a positive role in the progression of human malignant tumors. However, the molecular mechanism of SNHG1 remains elusive in breast cancer. RESULTS: LncRNA SNHG1 was upregulated and had a positive relationship with poor prognosis according to bioinformatics analysis in pan-cancer including breast cancer. Silencing SNHG1 inhibited tumorigenesis in breast cancer both in vitro and in vivo. Mechanistically, SNHG1 functioned as a competing endogenous RNA (ceRNA) to promote TERT expression by sponging miR-18b-5p in breast cancer. miR-18b-5p acted as a tumor repressor in breast cancer. Moreover, the combination of SNHG1 knockdown and TERT inhibitor administration showed a synergistic inhibitory effect on breast cancer growth in vivo. Finally, E2F1 as a transcription factor, binding to SNHG1 promoter and enhanced SNHG1 transcription in breast cancer. CONCLUSIONS: Our results provide a comprehensive understanding of the oncogenic mechanism of lncRNA SNHG1 in breast cancer. Importantly, we identified a novel E2F1–SNHG1–miR-18b-5p–TERT axis, which may be a potential therapeutic target for breast cancer. Our results also provided a potential treatment for breast cancer when knockdown SNHG1 and TERT inhibitor administration simultaneously. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13578-021-00675-5. BioMed Central 2021-08-31 /pmc/articles/PMC8407068/ /pubmed/34465388 http://dx.doi.org/10.1186/s13578-021-00675-5 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Kang, Yujuan
Wan, Lin
Wang, Qin
Yin, Yanling
Liu, Jiena
Liu, Lei
Wu, Hao
Zhang, Lei
Zhang, Xin
Xu, Shouping
Pang, Da
Long noncoding RNA SNHG1 promotes TERT expression by sponging miR-18b-5p in breast cancer
title Long noncoding RNA SNHG1 promotes TERT expression by sponging miR-18b-5p in breast cancer
title_full Long noncoding RNA SNHG1 promotes TERT expression by sponging miR-18b-5p in breast cancer
title_fullStr Long noncoding RNA SNHG1 promotes TERT expression by sponging miR-18b-5p in breast cancer
title_full_unstemmed Long noncoding RNA SNHG1 promotes TERT expression by sponging miR-18b-5p in breast cancer
title_short Long noncoding RNA SNHG1 promotes TERT expression by sponging miR-18b-5p in breast cancer
title_sort long noncoding rna snhg1 promotes tert expression by sponging mir-18b-5p in breast cancer
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8407068/
https://www.ncbi.nlm.nih.gov/pubmed/34465388
http://dx.doi.org/10.1186/s13578-021-00675-5
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