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Long noncoding RNA SNHG1 promotes TERT expression by sponging miR-18b-5p in breast cancer
BACKGROUND: Long noncoding RNA (lncRNA) small nucleolar RNA host gene 1 (SNHG1) plays a positive role in the progression of human malignant tumors. However, the molecular mechanism of SNHG1 remains elusive in breast cancer. RESULTS: LncRNA SNHG1 was upregulated and had a positive relationship with p...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8407068/ https://www.ncbi.nlm.nih.gov/pubmed/34465388 http://dx.doi.org/10.1186/s13578-021-00675-5 |
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author | Kang, Yujuan Wan, Lin Wang, Qin Yin, Yanling Liu, Jiena Liu, Lei Wu, Hao Zhang, Lei Zhang, Xin Xu, Shouping Pang, Da |
author_facet | Kang, Yujuan Wan, Lin Wang, Qin Yin, Yanling Liu, Jiena Liu, Lei Wu, Hao Zhang, Lei Zhang, Xin Xu, Shouping Pang, Da |
author_sort | Kang, Yujuan |
collection | PubMed |
description | BACKGROUND: Long noncoding RNA (lncRNA) small nucleolar RNA host gene 1 (SNHG1) plays a positive role in the progression of human malignant tumors. However, the molecular mechanism of SNHG1 remains elusive in breast cancer. RESULTS: LncRNA SNHG1 was upregulated and had a positive relationship with poor prognosis according to bioinformatics analysis in pan-cancer including breast cancer. Silencing SNHG1 inhibited tumorigenesis in breast cancer both in vitro and in vivo. Mechanistically, SNHG1 functioned as a competing endogenous RNA (ceRNA) to promote TERT expression by sponging miR-18b-5p in breast cancer. miR-18b-5p acted as a tumor repressor in breast cancer. Moreover, the combination of SNHG1 knockdown and TERT inhibitor administration showed a synergistic inhibitory effect on breast cancer growth in vivo. Finally, E2F1 as a transcription factor, binding to SNHG1 promoter and enhanced SNHG1 transcription in breast cancer. CONCLUSIONS: Our results provide a comprehensive understanding of the oncogenic mechanism of lncRNA SNHG1 in breast cancer. Importantly, we identified a novel E2F1–SNHG1–miR-18b-5p–TERT axis, which may be a potential therapeutic target for breast cancer. Our results also provided a potential treatment for breast cancer when knockdown SNHG1 and TERT inhibitor administration simultaneously. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13578-021-00675-5. |
format | Online Article Text |
id | pubmed-8407068 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-84070682021-09-01 Long noncoding RNA SNHG1 promotes TERT expression by sponging miR-18b-5p in breast cancer Kang, Yujuan Wan, Lin Wang, Qin Yin, Yanling Liu, Jiena Liu, Lei Wu, Hao Zhang, Lei Zhang, Xin Xu, Shouping Pang, Da Cell Biosci Research BACKGROUND: Long noncoding RNA (lncRNA) small nucleolar RNA host gene 1 (SNHG1) plays a positive role in the progression of human malignant tumors. However, the molecular mechanism of SNHG1 remains elusive in breast cancer. RESULTS: LncRNA SNHG1 was upregulated and had a positive relationship with poor prognosis according to bioinformatics analysis in pan-cancer including breast cancer. Silencing SNHG1 inhibited tumorigenesis in breast cancer both in vitro and in vivo. Mechanistically, SNHG1 functioned as a competing endogenous RNA (ceRNA) to promote TERT expression by sponging miR-18b-5p in breast cancer. miR-18b-5p acted as a tumor repressor in breast cancer. Moreover, the combination of SNHG1 knockdown and TERT inhibitor administration showed a synergistic inhibitory effect on breast cancer growth in vivo. Finally, E2F1 as a transcription factor, binding to SNHG1 promoter and enhanced SNHG1 transcription in breast cancer. CONCLUSIONS: Our results provide a comprehensive understanding of the oncogenic mechanism of lncRNA SNHG1 in breast cancer. Importantly, we identified a novel E2F1–SNHG1–miR-18b-5p–TERT axis, which may be a potential therapeutic target for breast cancer. Our results also provided a potential treatment for breast cancer when knockdown SNHG1 and TERT inhibitor administration simultaneously. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13578-021-00675-5. BioMed Central 2021-08-31 /pmc/articles/PMC8407068/ /pubmed/34465388 http://dx.doi.org/10.1186/s13578-021-00675-5 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Kang, Yujuan Wan, Lin Wang, Qin Yin, Yanling Liu, Jiena Liu, Lei Wu, Hao Zhang, Lei Zhang, Xin Xu, Shouping Pang, Da Long noncoding RNA SNHG1 promotes TERT expression by sponging miR-18b-5p in breast cancer |
title | Long noncoding RNA SNHG1 promotes TERT expression by sponging miR-18b-5p in breast cancer |
title_full | Long noncoding RNA SNHG1 promotes TERT expression by sponging miR-18b-5p in breast cancer |
title_fullStr | Long noncoding RNA SNHG1 promotes TERT expression by sponging miR-18b-5p in breast cancer |
title_full_unstemmed | Long noncoding RNA SNHG1 promotes TERT expression by sponging miR-18b-5p in breast cancer |
title_short | Long noncoding RNA SNHG1 promotes TERT expression by sponging miR-18b-5p in breast cancer |
title_sort | long noncoding rna snhg1 promotes tert expression by sponging mir-18b-5p in breast cancer |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8407068/ https://www.ncbi.nlm.nih.gov/pubmed/34465388 http://dx.doi.org/10.1186/s13578-021-00675-5 |
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