Phase gradient microscopy in thick tissue with oblique back-illumination

Phase contrast techniques, such as differential interference contrast (DIC) microscopy, are widely used to provide morphological images of unstained biological samples. The trans-illumination geometry required for these techniques restricts their application to thin samples. We introduce oblique bac...

Descripción completa

Detalles Bibliográficos
Autores principales: Ford, Tim N, Chu, Kengyeh K, Mertz, Jerome
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2012
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8407656/
https://www.ncbi.nlm.nih.gov/pubmed/23103879
http://dx.doi.org/10.1038/nmeth.2219
_version_ 1783746667358453760
author Ford, Tim N
Chu, Kengyeh K
Mertz, Jerome
author_facet Ford, Tim N
Chu, Kengyeh K
Mertz, Jerome
author_sort Ford, Tim N
collection PubMed
description Phase contrast techniques, such as differential interference contrast (DIC) microscopy, are widely used to provide morphological images of unstained biological samples. The trans-illumination geometry required for these techniques restricts their application to thin samples. We introduce oblique back-illumination microscopy (OBM), a method of collecting en face phase gradient images of thick scattering samples, enabling near video-rate in vivo phase imaging with a miniaturized probe suitable for endoscopy.
format Online
Article
Text
id pubmed-8407656
institution National Center for Biotechnology Information
language English
publishDate 2012
record_format MEDLINE/PubMed
spelling pubmed-84076562021-08-31 Phase gradient microscopy in thick tissue with oblique back-illumination Ford, Tim N Chu, Kengyeh K Mertz, Jerome Nat Methods Article Phase contrast techniques, such as differential interference contrast (DIC) microscopy, are widely used to provide morphological images of unstained biological samples. The trans-illumination geometry required for these techniques restricts their application to thin samples. We introduce oblique back-illumination microscopy (OBM), a method of collecting en face phase gradient images of thick scattering samples, enabling near video-rate in vivo phase imaging with a miniaturized probe suitable for endoscopy. 2012-10-28 2012-12 /pmc/articles/PMC8407656/ /pubmed/23103879 http://dx.doi.org/10.1038/nmeth.2219 Text en http://www.nature.com/authors/editorial_policies/license.html#termsUsers may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Ford, Tim N
Chu, Kengyeh K
Mertz, Jerome
Phase gradient microscopy in thick tissue with oblique back-illumination
title Phase gradient microscopy in thick tissue with oblique back-illumination
title_full Phase gradient microscopy in thick tissue with oblique back-illumination
title_fullStr Phase gradient microscopy in thick tissue with oblique back-illumination
title_full_unstemmed Phase gradient microscopy in thick tissue with oblique back-illumination
title_short Phase gradient microscopy in thick tissue with oblique back-illumination
title_sort phase gradient microscopy in thick tissue with oblique back-illumination
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8407656/
https://www.ncbi.nlm.nih.gov/pubmed/23103879
http://dx.doi.org/10.1038/nmeth.2219
work_keys_str_mv AT fordtimn phasegradientmicroscopyinthicktissuewithobliquebackillumination
AT chukengyehk phasegradientmicroscopyinthicktissuewithobliquebackillumination
AT mertzjerome phasegradientmicroscopyinthicktissuewithobliquebackillumination

Ejemplares similares