Cargando…

Fungal Extract of Lasiodiplodia pseudotheobromae IBRL OS-64 Inhibits the Growth of Skin Pathogenic Bacterium and Attenuates Biofilms of Methicillin-Resistant Staphylococcus aureus

BACKGROUND: The emergence of multidrug-resistant pathogens associated with biofilm formation can cause life-threatening infections to humans. Therefore, the present study aims to evaluate the effects of the fungal extract of Lasiodiplodia pseudotheobromae (L. pseudotheobromae) Industrial Biotechnolo...

Descripción completa

Detalles Bibliográficos
Autores principales: Jalil, Mohd Taufiq Mat, Ibrahim, Darah
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Penerbit Universiti Sains Malaysia 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8407792/
https://www.ncbi.nlm.nih.gov/pubmed/34512128
http://dx.doi.org/10.21315/mjms2021.28.4.4
_version_ 1783746699615797248
author Jalil, Mohd Taufiq Mat
Ibrahim, Darah
author_facet Jalil, Mohd Taufiq Mat
Ibrahim, Darah
author_sort Jalil, Mohd Taufiq Mat
collection PubMed
description BACKGROUND: The emergence of multidrug-resistant pathogens associated with biofilm formation can cause life-threatening infections to humans. Therefore, the present study aims to evaluate the effects of the fungal extract of Lasiodiplodia pseudotheobromae (L. pseudotheobromae) Industrial Biotechnology Research Laboratory (IBRL) OS-64 on bacterial cells and the biofilm formation of methicillin-resistant Staphylococcus aureus (MRSA). METHODS: Broth microdilution and semi-quantitative adherence assays were conducted to determine the anti-biofilm activity of the fungal extract. Light and scanning electron microscopy (SEM) analyses were performed to observe the effect of the fungal extract on biofilm formation by MRSA. RESULTS: The transmission electron microscopy (TEM) microphotographs showed that the bacterial cells were severely damaged upon 24 h exposure to the extract and displayed several symptoms such as cell shrinkage and breakage. Meanwhile, results from the antibiofilm study indicated the extract attenuated the initial and preformed biofilms of MRSA by 80.82% and 61.39%, respectively. The initial biofilm was more sensitive to the extract compared to the pre-formed biofilm, as evidenced by the light microscopy and SEM observations that demonstrated more severe bacterial cell damage on the initial biofilms compared to pre-formed biofilms. CONCLUSION: The ethyl acetate extract of L. pseudotheobromae IBRL OS-64 significantly inhibited bacterial cells growth and eliminated biofilm formation by MRSA.
format Online
Article
Text
id pubmed-8407792
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Penerbit Universiti Sains Malaysia
record_format MEDLINE/PubMed
spelling pubmed-84077922021-09-09 Fungal Extract of Lasiodiplodia pseudotheobromae IBRL OS-64 Inhibits the Growth of Skin Pathogenic Bacterium and Attenuates Biofilms of Methicillin-Resistant Staphylococcus aureus Jalil, Mohd Taufiq Mat Ibrahim, Darah Malays J Med Sci Original Article BACKGROUND: The emergence of multidrug-resistant pathogens associated with biofilm formation can cause life-threatening infections to humans. Therefore, the present study aims to evaluate the effects of the fungal extract of Lasiodiplodia pseudotheobromae (L. pseudotheobromae) Industrial Biotechnology Research Laboratory (IBRL) OS-64 on bacterial cells and the biofilm formation of methicillin-resistant Staphylococcus aureus (MRSA). METHODS: Broth microdilution and semi-quantitative adherence assays were conducted to determine the anti-biofilm activity of the fungal extract. Light and scanning electron microscopy (SEM) analyses were performed to observe the effect of the fungal extract on biofilm formation by MRSA. RESULTS: The transmission electron microscopy (TEM) microphotographs showed that the bacterial cells were severely damaged upon 24 h exposure to the extract and displayed several symptoms such as cell shrinkage and breakage. Meanwhile, results from the antibiofilm study indicated the extract attenuated the initial and preformed biofilms of MRSA by 80.82% and 61.39%, respectively. The initial biofilm was more sensitive to the extract compared to the pre-formed biofilm, as evidenced by the light microscopy and SEM observations that demonstrated more severe bacterial cell damage on the initial biofilms compared to pre-formed biofilms. CONCLUSION: The ethyl acetate extract of L. pseudotheobromae IBRL OS-64 significantly inhibited bacterial cells growth and eliminated biofilm formation by MRSA. Penerbit Universiti Sains Malaysia 2021-08 2021-08-26 /pmc/articles/PMC8407792/ /pubmed/34512128 http://dx.doi.org/10.21315/mjms2021.28.4.4 Text en © Penerbit Universiti Sains Malaysia, 2021 https://creativecommons.org/licenses/by/4.0/This work is licensed under the terms of the Creative Commons Attribution (CC BY) (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ).
spellingShingle Original Article
Jalil, Mohd Taufiq Mat
Ibrahim, Darah
Fungal Extract of Lasiodiplodia pseudotheobromae IBRL OS-64 Inhibits the Growth of Skin Pathogenic Bacterium and Attenuates Biofilms of Methicillin-Resistant Staphylococcus aureus
title Fungal Extract of Lasiodiplodia pseudotheobromae IBRL OS-64 Inhibits the Growth of Skin Pathogenic Bacterium and Attenuates Biofilms of Methicillin-Resistant Staphylococcus aureus
title_full Fungal Extract of Lasiodiplodia pseudotheobromae IBRL OS-64 Inhibits the Growth of Skin Pathogenic Bacterium and Attenuates Biofilms of Methicillin-Resistant Staphylococcus aureus
title_fullStr Fungal Extract of Lasiodiplodia pseudotheobromae IBRL OS-64 Inhibits the Growth of Skin Pathogenic Bacterium and Attenuates Biofilms of Methicillin-Resistant Staphylococcus aureus
title_full_unstemmed Fungal Extract of Lasiodiplodia pseudotheobromae IBRL OS-64 Inhibits the Growth of Skin Pathogenic Bacterium and Attenuates Biofilms of Methicillin-Resistant Staphylococcus aureus
title_short Fungal Extract of Lasiodiplodia pseudotheobromae IBRL OS-64 Inhibits the Growth of Skin Pathogenic Bacterium and Attenuates Biofilms of Methicillin-Resistant Staphylococcus aureus
title_sort fungal extract of lasiodiplodia pseudotheobromae ibrl os-64 inhibits the growth of skin pathogenic bacterium and attenuates biofilms of methicillin-resistant staphylococcus aureus
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8407792/
https://www.ncbi.nlm.nih.gov/pubmed/34512128
http://dx.doi.org/10.21315/mjms2021.28.4.4
work_keys_str_mv AT jalilmohdtaufiqmat fungalextractoflasiodiplodiapseudotheobromaeibrlos64inhibitsthegrowthofskinpathogenicbacteriumandattenuatesbiofilmsofmethicillinresistantstaphylococcusaureus
AT ibrahimdarah fungalextractoflasiodiplodiapseudotheobromaeibrlos64inhibitsthegrowthofskinpathogenicbacteriumandattenuatesbiofilmsofmethicillinresistantstaphylococcusaureus