Real-time 3D single-molecule localization microscopy analysis using lookup tables

Herein, we present a new algorithm for real-time analysis of 3D single molecule localization microscopy images with a small impact on fitting accuracy using lookup-tables with discrete xyz-positions. The algorithm realizes real-time visualization during acquisition. We demonstrate its performance on...

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Detalles Bibliográficos
Autores principales: Hauser, Fabian, Jacak, Jaroslaw
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Optical Society of America 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8407837/
https://www.ncbi.nlm.nih.gov/pubmed/34513235
http://dx.doi.org/10.1364/BOE.424016
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author Hauser, Fabian
Jacak, Jaroslaw
author_facet Hauser, Fabian
Jacak, Jaroslaw
author_sort Hauser, Fabian
collection PubMed
description Herein, we present a new algorithm for real-time analysis of 3D single molecule localization microscopy images with a small impact on fitting accuracy using lookup-tables with discrete xyz-positions. The algorithm realizes real-time visualization during acquisition. We demonstrate its performance on simulated and measured data. Additionally, combining real-time fitting with a feedback loop controlling the activation laser pulse keeps the number of emitters per image frame constant.
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spelling pubmed-84078372021-09-10 Real-time 3D single-molecule localization microscopy analysis using lookup tables Hauser, Fabian Jacak, Jaroslaw Biomed Opt Express Article Herein, we present a new algorithm for real-time analysis of 3D single molecule localization microscopy images with a small impact on fitting accuracy using lookup-tables with discrete xyz-positions. The algorithm realizes real-time visualization during acquisition. We demonstrate its performance on simulated and measured data. Additionally, combining real-time fitting with a feedback loop controlling the activation laser pulse keeps the number of emitters per image frame constant. Optical Society of America 2021-07-16 /pmc/articles/PMC8407837/ /pubmed/34513235 http://dx.doi.org/10.1364/BOE.424016 Text en Published by The Optical Society under the terms of the Creative Commons Attribution 4.0 License. Further distribution of this work must maintain attribution to the author(s) and the published article’s title, journal citation, and DOI. https://creativecommons.org/licenses/by/4.0/https://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Hauser, Fabian
Jacak, Jaroslaw
Real-time 3D single-molecule localization microscopy analysis using lookup tables
title Real-time 3D single-molecule localization microscopy analysis using lookup tables
title_full Real-time 3D single-molecule localization microscopy analysis using lookup tables
title_fullStr Real-time 3D single-molecule localization microscopy analysis using lookup tables
title_full_unstemmed Real-time 3D single-molecule localization microscopy analysis using lookup tables
title_short Real-time 3D single-molecule localization microscopy analysis using lookup tables
title_sort real-time 3d single-molecule localization microscopy analysis using lookup tables
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8407837/
https://www.ncbi.nlm.nih.gov/pubmed/34513235
http://dx.doi.org/10.1364/BOE.424016
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