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Seroprevalence and molecular diversity of Human Herpesvirus 8 among people living with HIV in Brazzaville, Congo

Human herpesvirus 8 (HHV8) is endemic in Africa, although studies of this infection are rare in Congo. We evaluated seroprevalence and HHV-8 diversity among people living with HIV. We included 353 patients receiving highly active antiretroviral therapy. Antibodies against HHV-8 latency-associated nu...

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Autores principales: Malonga, Gervillien Arnold, Jary, Aude, Leducq, Valentin, Moudiongui Mboungou Malanda, Dimitry, Boumba, Anicet Luc Magloire, Chicaud, Elodie, Malet, Isabelle, Calvez, Vincent, Peko, Jean Felix, Marcelin, Anne-Geneviève
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8408137/
https://www.ncbi.nlm.nih.gov/pubmed/34465868
http://dx.doi.org/10.1038/s41598-021-97070-4
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author Malonga, Gervillien Arnold
Jary, Aude
Leducq, Valentin
Moudiongui Mboungou Malanda, Dimitry
Boumba, Anicet Luc Magloire
Chicaud, Elodie
Malet, Isabelle
Calvez, Vincent
Peko, Jean Felix
Marcelin, Anne-Geneviève
author_facet Malonga, Gervillien Arnold
Jary, Aude
Leducq, Valentin
Moudiongui Mboungou Malanda, Dimitry
Boumba, Anicet Luc Magloire
Chicaud, Elodie
Malet, Isabelle
Calvez, Vincent
Peko, Jean Felix
Marcelin, Anne-Geneviève
author_sort Malonga, Gervillien Arnold
collection PubMed
description Human herpesvirus 8 (HHV8) is endemic in Africa, although studies of this infection are rare in Congo. We evaluated seroprevalence and HHV-8 diversity among people living with HIV. We included 353 patients receiving highly active antiretroviral therapy. Antibodies against HHV-8 latency-associated nuclear antigen were detected by indirect immunofluorescence. In HHV-8 positive patients, we performed HHV-8 quantification in blood and saliva by real-time PCR and typing by Sanger sequencing of K1 open reading frame. HHV-8 seroprevalence was 19%, being male (odd ratio [OR] = 1.741, [95% Confidence interval {CI}, 0.97–3.07]; p = 0.0581) and having multiple sex partners before HIV diagnosis (OR = 1.682, [CI 95%, 0.97–2.92]; p = 0.0629) tended to be associated with HHV-8 seropositivity. Of the 64 HHV-8 seropositive patients, HHV-8 DNA was detected in 10 (16%) in saliva, 6 (9%) in whole-blood and in 2 (3%) in both whole-blood and saliva. Three out of 6 HHV-8 strains were subtypes A5, 2 subtype B1 and 1 subtype C. HHV-8 seroprevalence was relatively low with more frequent carriage in men, associated with asymptomatic oral excretion and a predominance of subtype A5. These data tend to support the hypothesis of horizontal transmission in people living with HIV in Brazzaville.
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spelling pubmed-84081372021-09-01 Seroprevalence and molecular diversity of Human Herpesvirus 8 among people living with HIV in Brazzaville, Congo Malonga, Gervillien Arnold Jary, Aude Leducq, Valentin Moudiongui Mboungou Malanda, Dimitry Boumba, Anicet Luc Magloire Chicaud, Elodie Malet, Isabelle Calvez, Vincent Peko, Jean Felix Marcelin, Anne-Geneviève Sci Rep Article Human herpesvirus 8 (HHV8) is endemic in Africa, although studies of this infection are rare in Congo. We evaluated seroprevalence and HHV-8 diversity among people living with HIV. We included 353 patients receiving highly active antiretroviral therapy. Antibodies against HHV-8 latency-associated nuclear antigen were detected by indirect immunofluorescence. In HHV-8 positive patients, we performed HHV-8 quantification in blood and saliva by real-time PCR and typing by Sanger sequencing of K1 open reading frame. HHV-8 seroprevalence was 19%, being male (odd ratio [OR] = 1.741, [95% Confidence interval {CI}, 0.97–3.07]; p = 0.0581) and having multiple sex partners before HIV diagnosis (OR = 1.682, [CI 95%, 0.97–2.92]; p = 0.0629) tended to be associated with HHV-8 seropositivity. Of the 64 HHV-8 seropositive patients, HHV-8 DNA was detected in 10 (16%) in saliva, 6 (9%) in whole-blood and in 2 (3%) in both whole-blood and saliva. Three out of 6 HHV-8 strains were subtypes A5, 2 subtype B1 and 1 subtype C. HHV-8 seroprevalence was relatively low with more frequent carriage in men, associated with asymptomatic oral excretion and a predominance of subtype A5. These data tend to support the hypothesis of horizontal transmission in people living with HIV in Brazzaville. Nature Publishing Group UK 2021-08-31 /pmc/articles/PMC8408137/ /pubmed/34465868 http://dx.doi.org/10.1038/s41598-021-97070-4 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Malonga, Gervillien Arnold
Jary, Aude
Leducq, Valentin
Moudiongui Mboungou Malanda, Dimitry
Boumba, Anicet Luc Magloire
Chicaud, Elodie
Malet, Isabelle
Calvez, Vincent
Peko, Jean Felix
Marcelin, Anne-Geneviève
Seroprevalence and molecular diversity of Human Herpesvirus 8 among people living with HIV in Brazzaville, Congo
title Seroprevalence and molecular diversity of Human Herpesvirus 8 among people living with HIV in Brazzaville, Congo
title_full Seroprevalence and molecular diversity of Human Herpesvirus 8 among people living with HIV in Brazzaville, Congo
title_fullStr Seroprevalence and molecular diversity of Human Herpesvirus 8 among people living with HIV in Brazzaville, Congo
title_full_unstemmed Seroprevalence and molecular diversity of Human Herpesvirus 8 among people living with HIV in Brazzaville, Congo
title_short Seroprevalence and molecular diversity of Human Herpesvirus 8 among people living with HIV in Brazzaville, Congo
title_sort seroprevalence and molecular diversity of human herpesvirus 8 among people living with hiv in brazzaville, congo
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8408137/
https://www.ncbi.nlm.nih.gov/pubmed/34465868
http://dx.doi.org/10.1038/s41598-021-97070-4
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