Cargando…

Systematic morphological and morphometric analysis of identified olfactory receptor neurons in Drosophila melanogaster

The biophysical properties of sensory neurons are influenced by their morphometric and morphological features, whose precise measurements require high-quality volume electron microscopy (EM). However, systematic surveys of nanoscale characteristics for identified neurons are scarce. Here, we charact...

Descripción completa

Detalles Bibliográficos
Autores principales: Nava Gonzales, Cesar, McKaughan, Quintyn, Bushong, Eric A, Cauwenberghs, Kalyani, Ng, Renny, Madany, Matthew, Ellisman, Mark H, Su, Chih-Ying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8410077/
https://www.ncbi.nlm.nih.gov/pubmed/34423777
http://dx.doi.org/10.7554/eLife.69896
_version_ 1783747093204041728
author Nava Gonzales, Cesar
McKaughan, Quintyn
Bushong, Eric A
Cauwenberghs, Kalyani
Ng, Renny
Madany, Matthew
Ellisman, Mark H
Su, Chih-Ying
author_facet Nava Gonzales, Cesar
McKaughan, Quintyn
Bushong, Eric A
Cauwenberghs, Kalyani
Ng, Renny
Madany, Matthew
Ellisman, Mark H
Su, Chih-Ying
author_sort Nava Gonzales, Cesar
collection PubMed
description The biophysical properties of sensory neurons are influenced by their morphometric and morphological features, whose precise measurements require high-quality volume electron microscopy (EM). However, systematic surveys of nanoscale characteristics for identified neurons are scarce. Here, we characterize the morphology of Drosophila olfactory receptor neurons (ORNs) across the majority of genetically identified sensory hairs. By analyzing serial block-face electron microscopy images of cryofixed antennal tissues, we compile an extensive morphometric data set based on 122 reconstructed 3D models for 33 of the 40 identified antennal ORN types. Additionally, we observe multiple novel features—including extracellular vacuoles within sensillum lumen, intricate dendritic branching, mitochondria enrichment in select ORNs, novel sensillum types, and empty sensilla containing no neurons—which raise new questions pertinent to cell biology and sensory neurobiology. Our systematic survey is critical for future investigations into how the size and shape of sensory neurons influence their responses, sensitivity, and circuit function.
format Online
Article
Text
id pubmed-8410077
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher eLife Sciences Publications, Ltd
record_format MEDLINE/PubMed
spelling pubmed-84100772021-09-03 Systematic morphological and morphometric analysis of identified olfactory receptor neurons in Drosophila melanogaster Nava Gonzales, Cesar McKaughan, Quintyn Bushong, Eric A Cauwenberghs, Kalyani Ng, Renny Madany, Matthew Ellisman, Mark H Su, Chih-Ying eLife Neuroscience The biophysical properties of sensory neurons are influenced by their morphometric and morphological features, whose precise measurements require high-quality volume electron microscopy (EM). However, systematic surveys of nanoscale characteristics for identified neurons are scarce. Here, we characterize the morphology of Drosophila olfactory receptor neurons (ORNs) across the majority of genetically identified sensory hairs. By analyzing serial block-face electron microscopy images of cryofixed antennal tissues, we compile an extensive morphometric data set based on 122 reconstructed 3D models for 33 of the 40 identified antennal ORN types. Additionally, we observe multiple novel features—including extracellular vacuoles within sensillum lumen, intricate dendritic branching, mitochondria enrichment in select ORNs, novel sensillum types, and empty sensilla containing no neurons—which raise new questions pertinent to cell biology and sensory neurobiology. Our systematic survey is critical for future investigations into how the size and shape of sensory neurons influence their responses, sensitivity, and circuit function. eLife Sciences Publications, Ltd 2021-08-23 /pmc/articles/PMC8410077/ /pubmed/34423777 http://dx.doi.org/10.7554/eLife.69896 Text en © 2021, Nava Gonzales et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Neuroscience
Nava Gonzales, Cesar
McKaughan, Quintyn
Bushong, Eric A
Cauwenberghs, Kalyani
Ng, Renny
Madany, Matthew
Ellisman, Mark H
Su, Chih-Ying
Systematic morphological and morphometric analysis of identified olfactory receptor neurons in Drosophila melanogaster
title Systematic morphological and morphometric analysis of identified olfactory receptor neurons in Drosophila melanogaster
title_full Systematic morphological and morphometric analysis of identified olfactory receptor neurons in Drosophila melanogaster
title_fullStr Systematic morphological and morphometric analysis of identified olfactory receptor neurons in Drosophila melanogaster
title_full_unstemmed Systematic morphological and morphometric analysis of identified olfactory receptor neurons in Drosophila melanogaster
title_short Systematic morphological and morphometric analysis of identified olfactory receptor neurons in Drosophila melanogaster
title_sort systematic morphological and morphometric analysis of identified olfactory receptor neurons in drosophila melanogaster
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8410077/
https://www.ncbi.nlm.nih.gov/pubmed/34423777
http://dx.doi.org/10.7554/eLife.69896
work_keys_str_mv AT navagonzalescesar systematicmorphologicalandmorphometricanalysisofidentifiedolfactoryreceptorneuronsindrosophilamelanogaster
AT mckaughanquintyn systematicmorphologicalandmorphometricanalysisofidentifiedolfactoryreceptorneuronsindrosophilamelanogaster
AT bushongerica systematicmorphologicalandmorphometricanalysisofidentifiedolfactoryreceptorneuronsindrosophilamelanogaster
AT cauwenberghskalyani systematicmorphologicalandmorphometricanalysisofidentifiedolfactoryreceptorneuronsindrosophilamelanogaster
AT ngrenny systematicmorphologicalandmorphometricanalysisofidentifiedolfactoryreceptorneuronsindrosophilamelanogaster
AT madanymatthew systematicmorphologicalandmorphometricanalysisofidentifiedolfactoryreceptorneuronsindrosophilamelanogaster
AT ellismanmarkh systematicmorphologicalandmorphometricanalysisofidentifiedolfactoryreceptorneuronsindrosophilamelanogaster
AT suchihying systematicmorphologicalandmorphometricanalysisofidentifiedolfactoryreceptorneuronsindrosophilamelanogaster