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Systematic morphological and morphometric analysis of identified olfactory receptor neurons in Drosophila melanogaster
The biophysical properties of sensory neurons are influenced by their morphometric and morphological features, whose precise measurements require high-quality volume electron microscopy (EM). However, systematic surveys of nanoscale characteristics for identified neurons are scarce. Here, we charact...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8410077/ https://www.ncbi.nlm.nih.gov/pubmed/34423777 http://dx.doi.org/10.7554/eLife.69896 |
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author | Nava Gonzales, Cesar McKaughan, Quintyn Bushong, Eric A Cauwenberghs, Kalyani Ng, Renny Madany, Matthew Ellisman, Mark H Su, Chih-Ying |
author_facet | Nava Gonzales, Cesar McKaughan, Quintyn Bushong, Eric A Cauwenberghs, Kalyani Ng, Renny Madany, Matthew Ellisman, Mark H Su, Chih-Ying |
author_sort | Nava Gonzales, Cesar |
collection | PubMed |
description | The biophysical properties of sensory neurons are influenced by their morphometric and morphological features, whose precise measurements require high-quality volume electron microscopy (EM). However, systematic surveys of nanoscale characteristics for identified neurons are scarce. Here, we characterize the morphology of Drosophila olfactory receptor neurons (ORNs) across the majority of genetically identified sensory hairs. By analyzing serial block-face electron microscopy images of cryofixed antennal tissues, we compile an extensive morphometric data set based on 122 reconstructed 3D models for 33 of the 40 identified antennal ORN types. Additionally, we observe multiple novel features—including extracellular vacuoles within sensillum lumen, intricate dendritic branching, mitochondria enrichment in select ORNs, novel sensillum types, and empty sensilla containing no neurons—which raise new questions pertinent to cell biology and sensory neurobiology. Our systematic survey is critical for future investigations into how the size and shape of sensory neurons influence their responses, sensitivity, and circuit function. |
format | Online Article Text |
id | pubmed-8410077 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-84100772021-09-03 Systematic morphological and morphometric analysis of identified olfactory receptor neurons in Drosophila melanogaster Nava Gonzales, Cesar McKaughan, Quintyn Bushong, Eric A Cauwenberghs, Kalyani Ng, Renny Madany, Matthew Ellisman, Mark H Su, Chih-Ying eLife Neuroscience The biophysical properties of sensory neurons are influenced by their morphometric and morphological features, whose precise measurements require high-quality volume electron microscopy (EM). However, systematic surveys of nanoscale characteristics for identified neurons are scarce. Here, we characterize the morphology of Drosophila olfactory receptor neurons (ORNs) across the majority of genetically identified sensory hairs. By analyzing serial block-face electron microscopy images of cryofixed antennal tissues, we compile an extensive morphometric data set based on 122 reconstructed 3D models for 33 of the 40 identified antennal ORN types. Additionally, we observe multiple novel features—including extracellular vacuoles within sensillum lumen, intricate dendritic branching, mitochondria enrichment in select ORNs, novel sensillum types, and empty sensilla containing no neurons—which raise new questions pertinent to cell biology and sensory neurobiology. Our systematic survey is critical for future investigations into how the size and shape of sensory neurons influence their responses, sensitivity, and circuit function. eLife Sciences Publications, Ltd 2021-08-23 /pmc/articles/PMC8410077/ /pubmed/34423777 http://dx.doi.org/10.7554/eLife.69896 Text en © 2021, Nava Gonzales et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Neuroscience Nava Gonzales, Cesar McKaughan, Quintyn Bushong, Eric A Cauwenberghs, Kalyani Ng, Renny Madany, Matthew Ellisman, Mark H Su, Chih-Ying Systematic morphological and morphometric analysis of identified olfactory receptor neurons in Drosophila melanogaster |
title | Systematic morphological and morphometric analysis of identified olfactory receptor neurons in Drosophila melanogaster |
title_full | Systematic morphological and morphometric analysis of identified olfactory receptor neurons in Drosophila melanogaster |
title_fullStr | Systematic morphological and morphometric analysis of identified olfactory receptor neurons in Drosophila melanogaster |
title_full_unstemmed | Systematic morphological and morphometric analysis of identified olfactory receptor neurons in Drosophila melanogaster |
title_short | Systematic morphological and morphometric analysis of identified olfactory receptor neurons in Drosophila melanogaster |
title_sort | systematic morphological and morphometric analysis of identified olfactory receptor neurons in drosophila melanogaster |
topic | Neuroscience |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8410077/ https://www.ncbi.nlm.nih.gov/pubmed/34423777 http://dx.doi.org/10.7554/eLife.69896 |
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