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Non-respiratory particles emitted by guinea pigs in airborne disease transmission experiments
Animal models are often used to assess the airborne transmissibility of various pathogens, which are typically assumed to be carried by expiratory droplets emitted directly from the respiratory tract of the infected animal. We recently established that influenza virus is also transmissible via “aero...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8410799/ https://www.ncbi.nlm.nih.gov/pubmed/34471147 http://dx.doi.org/10.1038/s41598-021-96678-w |
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author | Asadi, Sima Tupas, Manilyn J. Barre, Ramya S. Wexler, Anthony S. Bouvier, Nicole M. Ristenpart, William D. |
author_facet | Asadi, Sima Tupas, Manilyn J. Barre, Ramya S. Wexler, Anthony S. Bouvier, Nicole M. Ristenpart, William D. |
author_sort | Asadi, Sima |
collection | PubMed |
description | Animal models are often used to assess the airborne transmissibility of various pathogens, which are typically assumed to be carried by expiratory droplets emitted directly from the respiratory tract of the infected animal. We recently established that influenza virus is also transmissible via “aerosolized fomites,” micron-scale dust particulates released from virus-contaminated surfaces (Asadi et al. in Nat Commun 11(1):4062, 2020). Here we expand on this observation, by counting and characterizing the particles emitted from guinea pig cages using an Aerodynamic Particle Sizer (APS) and an Interferometric Mie Imaging (IMI) system. Of over 9000 airborne particles emitted from guinea pig cages and directly imaged with IMI, none had an interference pattern indicative of a liquid droplet. Separate measurements of the particle count using the APS indicate that particle concentrations spike upwards immediately following animal motion, then decay exponentially with a time constant commensurate with the air exchange rate in the cage. Taken together, the results presented here raise the possibility that a non-negligible fraction of airborne influenza transmission events between guinea pigs occurs via aerosolized fomites rather than respiratory droplets, though the relative frequencies of these two routes have yet to be definitively determined. |
format | Online Article Text |
id | pubmed-8410799 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-84107992021-09-03 Non-respiratory particles emitted by guinea pigs in airborne disease transmission experiments Asadi, Sima Tupas, Manilyn J. Barre, Ramya S. Wexler, Anthony S. Bouvier, Nicole M. Ristenpart, William D. Sci Rep Article Animal models are often used to assess the airborne transmissibility of various pathogens, which are typically assumed to be carried by expiratory droplets emitted directly from the respiratory tract of the infected animal. We recently established that influenza virus is also transmissible via “aerosolized fomites,” micron-scale dust particulates released from virus-contaminated surfaces (Asadi et al. in Nat Commun 11(1):4062, 2020). Here we expand on this observation, by counting and characterizing the particles emitted from guinea pig cages using an Aerodynamic Particle Sizer (APS) and an Interferometric Mie Imaging (IMI) system. Of over 9000 airborne particles emitted from guinea pig cages and directly imaged with IMI, none had an interference pattern indicative of a liquid droplet. Separate measurements of the particle count using the APS indicate that particle concentrations spike upwards immediately following animal motion, then decay exponentially with a time constant commensurate with the air exchange rate in the cage. Taken together, the results presented here raise the possibility that a non-negligible fraction of airborne influenza transmission events between guinea pigs occurs via aerosolized fomites rather than respiratory droplets, though the relative frequencies of these two routes have yet to be definitively determined. Nature Publishing Group UK 2021-09-01 /pmc/articles/PMC8410799/ /pubmed/34471147 http://dx.doi.org/10.1038/s41598-021-96678-w Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Asadi, Sima Tupas, Manilyn J. Barre, Ramya S. Wexler, Anthony S. Bouvier, Nicole M. Ristenpart, William D. Non-respiratory particles emitted by guinea pigs in airborne disease transmission experiments |
title | Non-respiratory particles emitted by guinea pigs in airborne disease transmission experiments |
title_full | Non-respiratory particles emitted by guinea pigs in airborne disease transmission experiments |
title_fullStr | Non-respiratory particles emitted by guinea pigs in airborne disease transmission experiments |
title_full_unstemmed | Non-respiratory particles emitted by guinea pigs in airborne disease transmission experiments |
title_short | Non-respiratory particles emitted by guinea pigs in airborne disease transmission experiments |
title_sort | non-respiratory particles emitted by guinea pigs in airborne disease transmission experiments |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8410799/ https://www.ncbi.nlm.nih.gov/pubmed/34471147 http://dx.doi.org/10.1038/s41598-021-96678-w |
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