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Psychological stress induced bladder overactivity in female mice is associated with enhanced afferent nerve activity

Psychological stress has been linked to the development and exacerbation of overactive bladder symptoms, as well as afferent sensitisation in other organ systems. Therefore, we aimed to investigate the effects of water avoidance stress on bladder afferent nerve activity in response to bladder fillin...

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Autores principales: Mills, Kylie A., West, Eliza G., Sellers, Donna J., Chess-Williams, Russ, McDermott, Catherine
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8410840/
https://www.ncbi.nlm.nih.gov/pubmed/34471159
http://dx.doi.org/10.1038/s41598-021-97053-5
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author Mills, Kylie A.
West, Eliza G.
Sellers, Donna J.
Chess-Williams, Russ
McDermott, Catherine
author_facet Mills, Kylie A.
West, Eliza G.
Sellers, Donna J.
Chess-Williams, Russ
McDermott, Catherine
author_sort Mills, Kylie A.
collection PubMed
description Psychological stress has been linked to the development and exacerbation of overactive bladder symptoms, as well as afferent sensitisation in other organ systems. Therefore, we aimed to investigate the effects of water avoidance stress on bladder afferent nerve activity in response to bladder filling and pharmaceutical stimulation with carbachol and ATP in mice. Adult female C57BL/6J mice were exposed to either water avoidance stress (WAS) for 1 h/day for 10 days or normal housing conditions. Voiding behaviour was measured before starting and 24-h after final stress exposure and then animals were euthanised to measure afferent nerve activity in association with bladder compliance, spontaneous phasic activity, contractile responses, as well as release of urothelial mediators. WAS caused increased urinary frequency without affecting urine production. The afferent nerve activity at low bladder pressures (4–7 mmHg), relevant to normal physiological filling, was significantly increased after stress. Both low and high threshold nerves demonstrated enhanced activity at physiological bladder pressures. Urothelial ATP and acetylcholine release and bladder compliance were unaffected by stress as was the detrusor response to ATP (1 mM) and carbachol (1 µM). WAS caused enhanced activity of individual afferent nerve fibres in response bladder distension. The enhanced activity was seen in both low and high threshold nerves suggesting that stressed animals may experience enhanced bladder filling sensations at lower bladder volumes as well as increased pain sensations, both potentially contributing to the increased urinary frequency seen after stress.
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spelling pubmed-84108402021-09-03 Psychological stress induced bladder overactivity in female mice is associated with enhanced afferent nerve activity Mills, Kylie A. West, Eliza G. Sellers, Donna J. Chess-Williams, Russ McDermott, Catherine Sci Rep Article Psychological stress has been linked to the development and exacerbation of overactive bladder symptoms, as well as afferent sensitisation in other organ systems. Therefore, we aimed to investigate the effects of water avoidance stress on bladder afferent nerve activity in response to bladder filling and pharmaceutical stimulation with carbachol and ATP in mice. Adult female C57BL/6J mice were exposed to either water avoidance stress (WAS) for 1 h/day for 10 days or normal housing conditions. Voiding behaviour was measured before starting and 24-h after final stress exposure and then animals were euthanised to measure afferent nerve activity in association with bladder compliance, spontaneous phasic activity, contractile responses, as well as release of urothelial mediators. WAS caused increased urinary frequency without affecting urine production. The afferent nerve activity at low bladder pressures (4–7 mmHg), relevant to normal physiological filling, was significantly increased after stress. Both low and high threshold nerves demonstrated enhanced activity at physiological bladder pressures. Urothelial ATP and acetylcholine release and bladder compliance were unaffected by stress as was the detrusor response to ATP (1 mM) and carbachol (1 µM). WAS caused enhanced activity of individual afferent nerve fibres in response bladder distension. The enhanced activity was seen in both low and high threshold nerves suggesting that stressed animals may experience enhanced bladder filling sensations at lower bladder volumes as well as increased pain sensations, both potentially contributing to the increased urinary frequency seen after stress. Nature Publishing Group UK 2021-09-01 /pmc/articles/PMC8410840/ /pubmed/34471159 http://dx.doi.org/10.1038/s41598-021-97053-5 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Mills, Kylie A.
West, Eliza G.
Sellers, Donna J.
Chess-Williams, Russ
McDermott, Catherine
Psychological stress induced bladder overactivity in female mice is associated with enhanced afferent nerve activity
title Psychological stress induced bladder overactivity in female mice is associated with enhanced afferent nerve activity
title_full Psychological stress induced bladder overactivity in female mice is associated with enhanced afferent nerve activity
title_fullStr Psychological stress induced bladder overactivity in female mice is associated with enhanced afferent nerve activity
title_full_unstemmed Psychological stress induced bladder overactivity in female mice is associated with enhanced afferent nerve activity
title_short Psychological stress induced bladder overactivity in female mice is associated with enhanced afferent nerve activity
title_sort psychological stress induced bladder overactivity in female mice is associated with enhanced afferent nerve activity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8410840/
https://www.ncbi.nlm.nih.gov/pubmed/34471159
http://dx.doi.org/10.1038/s41598-021-97053-5
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