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Altered Microbiomes in Thirdhand Smoke-Exposed Children and their Home Environments

INTRODUCTION. Tobacco smoke contains numerous toxic chemicals that accumulate in indoor environments creating thirdhand smoke (THS). We investigated if THS-polluted homes differed in children’s human and built environment microbiomes as compared to THS-free homes. METHODS. Participants were N=19 THS...

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Autores principales: Kelley, Scott T., Liu, William, Quintana, Penelope J.E., Hoh, Eunha, Dodder, Nathan G., Mahabee-Gittens, E. Melinda, Padilla, Samuel, Ogden, Shawn, Frenzel, Sia, Sisk-Hackworth, Laura, Matt, Georg E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8410873/
https://www.ncbi.nlm.nih.gov/pubmed/33654287
http://dx.doi.org/10.1038/s41390-021-01400-1
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author Kelley, Scott T.
Liu, William
Quintana, Penelope J.E.
Hoh, Eunha
Dodder, Nathan G.
Mahabee-Gittens, E. Melinda
Padilla, Samuel
Ogden, Shawn
Frenzel, Sia
Sisk-Hackworth, Laura
Matt, Georg E.
author_facet Kelley, Scott T.
Liu, William
Quintana, Penelope J.E.
Hoh, Eunha
Dodder, Nathan G.
Mahabee-Gittens, E. Melinda
Padilla, Samuel
Ogden, Shawn
Frenzel, Sia
Sisk-Hackworth, Laura
Matt, Georg E.
author_sort Kelley, Scott T.
collection PubMed
description INTRODUCTION. Tobacco smoke contains numerous toxic chemicals that accumulate in indoor environments creating thirdhand smoke (THS). We investigated if THS-polluted homes differed in children’s human and built environment microbiomes as compared to THS-free homes. METHODS. Participants were N=19 THS exposed children and N=10 unexposed children (≤ 5 years) and their parents. Environmental and biological samples were analyzed for THS pollutants and exposure. Swab samples were collected from the built environment (floor, table, armrest, bedframe) and child (finger, nose, mouth, and ear canal) and 16S ribosomal RNA genes were analyzed for bacterial taxa using high-throughput DNA sequencing. RESULTS. Phylogenetic α-diversity was significantly higher for the built environmental microbiomes in THS-polluted homes compared to THS-free homes (p<0.014). Log2 fold comparison found differences between THS-polluted and THS-free homes for specific genera at built environment (e.g., Acinetobacter, Bradyrhizobium, Corynebacterium, Gemella, Neisseria, Staphylococcus, Streptococcus, Veillonella) and in samples from children s (esp. Corynebacterium, Gemella, Lautropia, Neisseria, Rothia, Staphylococcus, and Veillonella). CONCLUSION. When exposed to THS, indoor and children microbiomes are altered in an environment-specific manner. Changes are similar to those reported in previous studies for smokers and secondhand smoke-exposed persons. THS-induced changes in child and environmental microbiome may play a role in clinical outcomes in children.
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spelling pubmed-84108732021-12-17 Altered Microbiomes in Thirdhand Smoke-Exposed Children and their Home Environments Kelley, Scott T. Liu, William Quintana, Penelope J.E. Hoh, Eunha Dodder, Nathan G. Mahabee-Gittens, E. Melinda Padilla, Samuel Ogden, Shawn Frenzel, Sia Sisk-Hackworth, Laura Matt, Georg E. Pediatr Res Article INTRODUCTION. Tobacco smoke contains numerous toxic chemicals that accumulate in indoor environments creating thirdhand smoke (THS). We investigated if THS-polluted homes differed in children’s human and built environment microbiomes as compared to THS-free homes. METHODS. Participants were N=19 THS exposed children and N=10 unexposed children (≤ 5 years) and their parents. Environmental and biological samples were analyzed for THS pollutants and exposure. Swab samples were collected from the built environment (floor, table, armrest, bedframe) and child (finger, nose, mouth, and ear canal) and 16S ribosomal RNA genes were analyzed for bacterial taxa using high-throughput DNA sequencing. RESULTS. Phylogenetic α-diversity was significantly higher for the built environmental microbiomes in THS-polluted homes compared to THS-free homes (p<0.014). Log2 fold comparison found differences between THS-polluted and THS-free homes for specific genera at built environment (e.g., Acinetobacter, Bradyrhizobium, Corynebacterium, Gemella, Neisseria, Staphylococcus, Streptococcus, Veillonella) and in samples from children s (esp. Corynebacterium, Gemella, Lautropia, Neisseria, Rothia, Staphylococcus, and Veillonella). CONCLUSION. When exposed to THS, indoor and children microbiomes are altered in an environment-specific manner. Changes are similar to those reported in previous studies for smokers and secondhand smoke-exposed persons. THS-induced changes in child and environmental microbiome may play a role in clinical outcomes in children. 2021-03-02 2021-12 /pmc/articles/PMC8410873/ /pubmed/33654287 http://dx.doi.org/10.1038/s41390-021-01400-1 Text en http://www.nature.com/authors/editorial_policies/license.html#termsUsers may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Kelley, Scott T.
Liu, William
Quintana, Penelope J.E.
Hoh, Eunha
Dodder, Nathan G.
Mahabee-Gittens, E. Melinda
Padilla, Samuel
Ogden, Shawn
Frenzel, Sia
Sisk-Hackworth, Laura
Matt, Georg E.
Altered Microbiomes in Thirdhand Smoke-Exposed Children and their Home Environments
title Altered Microbiomes in Thirdhand Smoke-Exposed Children and their Home Environments
title_full Altered Microbiomes in Thirdhand Smoke-Exposed Children and their Home Environments
title_fullStr Altered Microbiomes in Thirdhand Smoke-Exposed Children and their Home Environments
title_full_unstemmed Altered Microbiomes in Thirdhand Smoke-Exposed Children and their Home Environments
title_short Altered Microbiomes in Thirdhand Smoke-Exposed Children and their Home Environments
title_sort altered microbiomes in thirdhand smoke-exposed children and their home environments
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8410873/
https://www.ncbi.nlm.nih.gov/pubmed/33654287
http://dx.doi.org/10.1038/s41390-021-01400-1
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