Cargando…

Pro- and anti-inflammatory macrophages express a sub-type specific purinergic receptor profile

Extracellular nucleotides act as danger signals that orchestrate inflammation by purinergic receptor activation. The expression pattern of different purinergic receptors may correlate with a pro- or anti-inflammatory phenotype. Macrophages function as pro-inflammatory M1 macrophages (M1) or anti-inf...

Descripción completa

Detalles Bibliográficos
Autores principales: Merz, J., Nettesheim, A., von Garlen, S., Albrecht, P., Saller, B. S., Engelmann, J., Hertle, L., Schäfer, I., Dimanski, D., König, S., Karnbrock, L., Bulatova, K., Peikert, A., Hoppe, N., Hilgendorf, I., von zur Mühlen, C., Wolf, D., Groß, O., Bode, C., Zirlik, A., Stachon, P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8410913/
https://www.ncbi.nlm.nih.gov/pubmed/34282551
http://dx.doi.org/10.1007/s11302-021-09798-3
_version_ 1783747196110241792
author Merz, J.
Nettesheim, A.
von Garlen, S.
Albrecht, P.
Saller, B. S.
Engelmann, J.
Hertle, L.
Schäfer, I.
Dimanski, D.
König, S.
Karnbrock, L.
Bulatova, K.
Peikert, A.
Hoppe, N.
Hilgendorf, I.
von zur Mühlen, C.
Wolf, D.
Groß, O.
Bode, C.
Zirlik, A.
Stachon, P.
author_facet Merz, J.
Nettesheim, A.
von Garlen, S.
Albrecht, P.
Saller, B. S.
Engelmann, J.
Hertle, L.
Schäfer, I.
Dimanski, D.
König, S.
Karnbrock, L.
Bulatova, K.
Peikert, A.
Hoppe, N.
Hilgendorf, I.
von zur Mühlen, C.
Wolf, D.
Groß, O.
Bode, C.
Zirlik, A.
Stachon, P.
author_sort Merz, J.
collection PubMed
description Extracellular nucleotides act as danger signals that orchestrate inflammation by purinergic receptor activation. The expression pattern of different purinergic receptors may correlate with a pro- or anti-inflammatory phenotype. Macrophages function as pro-inflammatory M1 macrophages (M1) or anti-inflammatory M2 macrophages (M2). The present study found that murine bone marrow-derived macrophages express a unique purinergic receptor profile during in vitro polarization. As assessed by real-time polymerase chain reaction (PCR), Gαs-coupled P1 receptors A2A and A2B are upregulated in M1 and M2 compared to M0, but A2A 15 times higher in M1. The ionotropic P2 receptor P2X(5) is selectively upregulated in M1- and M2-polarized macrophages. P2X(7) is temporarily expressed in M1 macrophages. Metabotropic P2Y receptors showed a distinct expression profile in M1 and M2-polarized macrophages: Gαq coupled P2Y(1) and P2Y(6) are exclusively upregulated in M2, whereas Gαi P2Y(13) and P2Y(14) are overexpressed in M1. This consequently leads to functional differences between M1 and M2 in response to adenosine di-phosphate stimulation (ADP): In contrast to M1, M2 showed increased cytoplasmatic calcium after ADP stimulation. In the present study we show that bone marrow-derived macrophages express a unique repertoire of purinergic receptors. We show for the first time that the repertoire of purinergic receptors is highly flexible and quickly adapts upon pro- and anti-inflammatory macrophage differentiation with functional consequences to nucleotide stimulation.
format Online
Article
Text
id pubmed-8410913
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Springer Netherlands
record_format MEDLINE/PubMed
spelling pubmed-84109132021-09-16 Pro- and anti-inflammatory macrophages express a sub-type specific purinergic receptor profile Merz, J. Nettesheim, A. von Garlen, S. Albrecht, P. Saller, B. S. Engelmann, J. Hertle, L. Schäfer, I. Dimanski, D. König, S. Karnbrock, L. Bulatova, K. Peikert, A. Hoppe, N. Hilgendorf, I. von zur Mühlen, C. Wolf, D. Groß, O. Bode, C. Zirlik, A. Stachon, P. Purinergic Signal Original Article Extracellular nucleotides act as danger signals that orchestrate inflammation by purinergic receptor activation. The expression pattern of different purinergic receptors may correlate with a pro- or anti-inflammatory phenotype. Macrophages function as pro-inflammatory M1 macrophages (M1) or anti-inflammatory M2 macrophages (M2). The present study found that murine bone marrow-derived macrophages express a unique purinergic receptor profile during in vitro polarization. As assessed by real-time polymerase chain reaction (PCR), Gαs-coupled P1 receptors A2A and A2B are upregulated in M1 and M2 compared to M0, but A2A 15 times higher in M1. The ionotropic P2 receptor P2X(5) is selectively upregulated in M1- and M2-polarized macrophages. P2X(7) is temporarily expressed in M1 macrophages. Metabotropic P2Y receptors showed a distinct expression profile in M1 and M2-polarized macrophages: Gαq coupled P2Y(1) and P2Y(6) are exclusively upregulated in M2, whereas Gαi P2Y(13) and P2Y(14) are overexpressed in M1. This consequently leads to functional differences between M1 and M2 in response to adenosine di-phosphate stimulation (ADP): In contrast to M1, M2 showed increased cytoplasmatic calcium after ADP stimulation. In the present study we show that bone marrow-derived macrophages express a unique repertoire of purinergic receptors. We show for the first time that the repertoire of purinergic receptors is highly flexible and quickly adapts upon pro- and anti-inflammatory macrophage differentiation with functional consequences to nucleotide stimulation. Springer Netherlands 2021-07-20 2021-09 /pmc/articles/PMC8410913/ /pubmed/34282551 http://dx.doi.org/10.1007/s11302-021-09798-3 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Original Article
Merz, J.
Nettesheim, A.
von Garlen, S.
Albrecht, P.
Saller, B. S.
Engelmann, J.
Hertle, L.
Schäfer, I.
Dimanski, D.
König, S.
Karnbrock, L.
Bulatova, K.
Peikert, A.
Hoppe, N.
Hilgendorf, I.
von zur Mühlen, C.
Wolf, D.
Groß, O.
Bode, C.
Zirlik, A.
Stachon, P.
Pro- and anti-inflammatory macrophages express a sub-type specific purinergic receptor profile
title Pro- and anti-inflammatory macrophages express a sub-type specific purinergic receptor profile
title_full Pro- and anti-inflammatory macrophages express a sub-type specific purinergic receptor profile
title_fullStr Pro- and anti-inflammatory macrophages express a sub-type specific purinergic receptor profile
title_full_unstemmed Pro- and anti-inflammatory macrophages express a sub-type specific purinergic receptor profile
title_short Pro- and anti-inflammatory macrophages express a sub-type specific purinergic receptor profile
title_sort pro- and anti-inflammatory macrophages express a sub-type specific purinergic receptor profile
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8410913/
https://www.ncbi.nlm.nih.gov/pubmed/34282551
http://dx.doi.org/10.1007/s11302-021-09798-3
work_keys_str_mv AT merzj proandantiinflammatorymacrophagesexpressasubtypespecificpurinergicreceptorprofile
AT nettesheima proandantiinflammatorymacrophagesexpressasubtypespecificpurinergicreceptorprofile
AT vongarlens proandantiinflammatorymacrophagesexpressasubtypespecificpurinergicreceptorprofile
AT albrechtp proandantiinflammatorymacrophagesexpressasubtypespecificpurinergicreceptorprofile
AT sallerbs proandantiinflammatorymacrophagesexpressasubtypespecificpurinergicreceptorprofile
AT engelmannj proandantiinflammatorymacrophagesexpressasubtypespecificpurinergicreceptorprofile
AT hertlel proandantiinflammatorymacrophagesexpressasubtypespecificpurinergicreceptorprofile
AT schaferi proandantiinflammatorymacrophagesexpressasubtypespecificpurinergicreceptorprofile
AT dimanskid proandantiinflammatorymacrophagesexpressasubtypespecificpurinergicreceptorprofile
AT konigs proandantiinflammatorymacrophagesexpressasubtypespecificpurinergicreceptorprofile
AT karnbrockl proandantiinflammatorymacrophagesexpressasubtypespecificpurinergicreceptorprofile
AT bulatovak proandantiinflammatorymacrophagesexpressasubtypespecificpurinergicreceptorprofile
AT peikerta proandantiinflammatorymacrophagesexpressasubtypespecificpurinergicreceptorprofile
AT hoppen proandantiinflammatorymacrophagesexpressasubtypespecificpurinergicreceptorprofile
AT hilgendorfi proandantiinflammatorymacrophagesexpressasubtypespecificpurinergicreceptorprofile
AT vonzurmuhlenc proandantiinflammatorymacrophagesexpressasubtypespecificpurinergicreceptorprofile
AT wolfd proandantiinflammatorymacrophagesexpressasubtypespecificpurinergicreceptorprofile
AT großo proandantiinflammatorymacrophagesexpressasubtypespecificpurinergicreceptorprofile
AT bodec proandantiinflammatorymacrophagesexpressasubtypespecificpurinergicreceptorprofile
AT zirlika proandantiinflammatorymacrophagesexpressasubtypespecificpurinergicreceptorprofile
AT stachonp proandantiinflammatorymacrophagesexpressasubtypespecificpurinergicreceptorprofile