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Tissue-specific DamID protocol using nanopore sequencing

DNA adenine methylation identification (DamID) is a powerful method to determine DNA binding profiles of proteins at a genomic scale. The method leverages the fusion between a protein of interest and the Dam methyltransferase of E. coli, which methylates proximal DNA in vivo. Here, we present an opt...

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Detalles Bibliográficos
Autores principales:	Gómez-Saldivar, Georgina, Glauser, Dominique A., Meister, Peter
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Journal of Biological Methods 2021
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8411031/ https://www.ncbi.nlm.nih.gov/pubmed/34514013 http://dx.doi.org/10.14440/jbm.2021.362

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