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Proteomic response and molecular regulatory mechanisms of Bacillus cereus spores under ultrasound treatment

This study was aimed at providing new insights on the proteomic response of bacterial spores to ultrasound. Data-independent-acquisition method was used to quantify the proteome change of Bacillus cereus spores after ultrasound treatment (200 W). This study revealed that 2485 proteins were extracted...

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Autores principales: Lv, Ruiling, Liu, Donghong, Wang, Wenjun, Xu, Enbo, Ding, Tian, Ye, Xingqian, Zhou, Jianwei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8411229/
https://www.ncbi.nlm.nih.gov/pubmed/34474268
http://dx.doi.org/10.1016/j.ultsonch.2021.105732
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author Lv, Ruiling
Liu, Donghong
Wang, Wenjun
Xu, Enbo
Ding, Tian
Ye, Xingqian
Zhou, Jianwei
author_facet Lv, Ruiling
Liu, Donghong
Wang, Wenjun
Xu, Enbo
Ding, Tian
Ye, Xingqian
Zhou, Jianwei
author_sort Lv, Ruiling
collection PubMed
description This study was aimed at providing new insights on the proteomic response of bacterial spores to ultrasound. Data-independent-acquisition method was used to quantify the proteome change of Bacillus cereus spores after ultrasound treatment (200 W). This study revealed that 2485 proteins were extracted from Bacillus cereus spores, most of them were related to metabolism. After ultrasound treatment, the expression of 340 proteins were significantly changed (the fold change ≥ 2 and p < 0.05), of which 207 proteins were significantly down-regulated. KEGG pathway analysis showed that differentially expressed proteins mainly distributed in metabolism pathway, cell process pathway and genetic information processing pathway after ultrasound treatment. Furthermore, this study analyzed the differentially expressed proteins in significant enrichment pathways. In particular, the expression of key proteins in the phosphorylation reaction of spores was significantly decreased after ultrasound treatment. Thus, ATP synthesis rate decreased and the phosphorylation reaction inhibited. Also, the decrease of the expression of key proteins related to the tricarboxylic acid cycle led to the decrease of nutrients metabolism of spores. Ultrasound treatment induced the down-regulation of fatty acid synthetase expression and promoted fatty acid metabolism at the same time. The content of fatty acids decreased in spores consequently.
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spelling pubmed-84112292021-09-08 Proteomic response and molecular regulatory mechanisms of Bacillus cereus spores under ultrasound treatment Lv, Ruiling Liu, Donghong Wang, Wenjun Xu, Enbo Ding, Tian Ye, Xingqian Zhou, Jianwei Ultrason Sonochem Original Research Article This study was aimed at providing new insights on the proteomic response of bacterial spores to ultrasound. Data-independent-acquisition method was used to quantify the proteome change of Bacillus cereus spores after ultrasound treatment (200 W). This study revealed that 2485 proteins were extracted from Bacillus cereus spores, most of them were related to metabolism. After ultrasound treatment, the expression of 340 proteins were significantly changed (the fold change ≥ 2 and p < 0.05), of which 207 proteins were significantly down-regulated. KEGG pathway analysis showed that differentially expressed proteins mainly distributed in metabolism pathway, cell process pathway and genetic information processing pathway after ultrasound treatment. Furthermore, this study analyzed the differentially expressed proteins in significant enrichment pathways. In particular, the expression of key proteins in the phosphorylation reaction of spores was significantly decreased after ultrasound treatment. Thus, ATP synthesis rate decreased and the phosphorylation reaction inhibited. Also, the decrease of the expression of key proteins related to the tricarboxylic acid cycle led to the decrease of nutrients metabolism of spores. Ultrasound treatment induced the down-regulation of fatty acid synthetase expression and promoted fatty acid metabolism at the same time. The content of fatty acids decreased in spores consequently. Elsevier 2021-08-21 /pmc/articles/PMC8411229/ /pubmed/34474268 http://dx.doi.org/10.1016/j.ultsonch.2021.105732 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Research Article
Lv, Ruiling
Liu, Donghong
Wang, Wenjun
Xu, Enbo
Ding, Tian
Ye, Xingqian
Zhou, Jianwei
Proteomic response and molecular regulatory mechanisms of Bacillus cereus spores under ultrasound treatment
title Proteomic response and molecular regulatory mechanisms of Bacillus cereus spores under ultrasound treatment
title_full Proteomic response and molecular regulatory mechanisms of Bacillus cereus spores under ultrasound treatment
title_fullStr Proteomic response and molecular regulatory mechanisms of Bacillus cereus spores under ultrasound treatment
title_full_unstemmed Proteomic response and molecular regulatory mechanisms of Bacillus cereus spores under ultrasound treatment
title_short Proteomic response and molecular regulatory mechanisms of Bacillus cereus spores under ultrasound treatment
title_sort proteomic response and molecular regulatory mechanisms of bacillus cereus spores under ultrasound treatment
topic Original Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8411229/
https://www.ncbi.nlm.nih.gov/pubmed/34474268
http://dx.doi.org/10.1016/j.ultsonch.2021.105732
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