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Proteomic response and molecular regulatory mechanisms of Bacillus cereus spores under ultrasound treatment
This study was aimed at providing new insights on the proteomic response of bacterial spores to ultrasound. Data-independent-acquisition method was used to quantify the proteome change of Bacillus cereus spores after ultrasound treatment (200 W). This study revealed that 2485 proteins were extracted...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8411229/ https://www.ncbi.nlm.nih.gov/pubmed/34474268 http://dx.doi.org/10.1016/j.ultsonch.2021.105732 |
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author | Lv, Ruiling Liu, Donghong Wang, Wenjun Xu, Enbo Ding, Tian Ye, Xingqian Zhou, Jianwei |
author_facet | Lv, Ruiling Liu, Donghong Wang, Wenjun Xu, Enbo Ding, Tian Ye, Xingqian Zhou, Jianwei |
author_sort | Lv, Ruiling |
collection | PubMed |
description | This study was aimed at providing new insights on the proteomic response of bacterial spores to ultrasound. Data-independent-acquisition method was used to quantify the proteome change of Bacillus cereus spores after ultrasound treatment (200 W). This study revealed that 2485 proteins were extracted from Bacillus cereus spores, most of them were related to metabolism. After ultrasound treatment, the expression of 340 proteins were significantly changed (the fold change ≥ 2 and p < 0.05), of which 207 proteins were significantly down-regulated. KEGG pathway analysis showed that differentially expressed proteins mainly distributed in metabolism pathway, cell process pathway and genetic information processing pathway after ultrasound treatment. Furthermore, this study analyzed the differentially expressed proteins in significant enrichment pathways. In particular, the expression of key proteins in the phosphorylation reaction of spores was significantly decreased after ultrasound treatment. Thus, ATP synthesis rate decreased and the phosphorylation reaction inhibited. Also, the decrease of the expression of key proteins related to the tricarboxylic acid cycle led to the decrease of nutrients metabolism of spores. Ultrasound treatment induced the down-regulation of fatty acid synthetase expression and promoted fatty acid metabolism at the same time. The content of fatty acids decreased in spores consequently. |
format | Online Article Text |
id | pubmed-8411229 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-84112292021-09-08 Proteomic response and molecular regulatory mechanisms of Bacillus cereus spores under ultrasound treatment Lv, Ruiling Liu, Donghong Wang, Wenjun Xu, Enbo Ding, Tian Ye, Xingqian Zhou, Jianwei Ultrason Sonochem Original Research Article This study was aimed at providing new insights on the proteomic response of bacterial spores to ultrasound. Data-independent-acquisition method was used to quantify the proteome change of Bacillus cereus spores after ultrasound treatment (200 W). This study revealed that 2485 proteins were extracted from Bacillus cereus spores, most of them were related to metabolism. After ultrasound treatment, the expression of 340 proteins were significantly changed (the fold change ≥ 2 and p < 0.05), of which 207 proteins were significantly down-regulated. KEGG pathway analysis showed that differentially expressed proteins mainly distributed in metabolism pathway, cell process pathway and genetic information processing pathway after ultrasound treatment. Furthermore, this study analyzed the differentially expressed proteins in significant enrichment pathways. In particular, the expression of key proteins in the phosphorylation reaction of spores was significantly decreased after ultrasound treatment. Thus, ATP synthesis rate decreased and the phosphorylation reaction inhibited. Also, the decrease of the expression of key proteins related to the tricarboxylic acid cycle led to the decrease of nutrients metabolism of spores. Ultrasound treatment induced the down-regulation of fatty acid synthetase expression and promoted fatty acid metabolism at the same time. The content of fatty acids decreased in spores consequently. Elsevier 2021-08-21 /pmc/articles/PMC8411229/ /pubmed/34474268 http://dx.doi.org/10.1016/j.ultsonch.2021.105732 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Original Research Article Lv, Ruiling Liu, Donghong Wang, Wenjun Xu, Enbo Ding, Tian Ye, Xingqian Zhou, Jianwei Proteomic response and molecular regulatory mechanisms of Bacillus cereus spores under ultrasound treatment |
title | Proteomic response and molecular regulatory mechanisms of Bacillus cereus spores under ultrasound treatment |
title_full | Proteomic response and molecular regulatory mechanisms of Bacillus cereus spores under ultrasound treatment |
title_fullStr | Proteomic response and molecular regulatory mechanisms of Bacillus cereus spores under ultrasound treatment |
title_full_unstemmed | Proteomic response and molecular regulatory mechanisms of Bacillus cereus spores under ultrasound treatment |
title_short | Proteomic response and molecular regulatory mechanisms of Bacillus cereus spores under ultrasound treatment |
title_sort | proteomic response and molecular regulatory mechanisms of bacillus cereus spores under ultrasound treatment |
topic | Original Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8411229/ https://www.ncbi.nlm.nih.gov/pubmed/34474268 http://dx.doi.org/10.1016/j.ultsonch.2021.105732 |
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