Kinetic analysis of Cas12a and Cas13a RNA-Guided nucleases for development of improved CRISPR-Based diagnostics

Bacterial CRISPR systems provide acquired immunity against invading nucleic acids by activating RNA-programmable RNases and DNases. Cas13a and Cas12a enzymes bound to CRISPR RNA (crRNA) recognize specific nucleic acid targets, initiating cleavage of the targets as well as non-target (trans) nucleic...

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Detalles Bibliográficos
Autores principales: Nalefski, Eric A., Patel, Nidhi, Leung, Philip J.Y., Islam, Zeba, Kooistra, Remy M., Parikh, Ishira, Marion, Estelle, Knott, Gavin J., Doudna, Jennifer A., Le Ny, Anne-Laure M., Madan, Damian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8411246/
https://www.ncbi.nlm.nih.gov/pubmed/34505008
http://dx.doi.org/10.1016/j.isci.2021.102996

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