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Differences in gene expression and variable splicing events of ovaries between large and small litter size in Chinese Xiang pigs
BACKGROUND: Although lots of quantitative trait loci (QTLs) and genes present roles in litter size of some breeds, the information might not make it clear for the huge diversity of reproductive capability in pig breeds. To elucidate the inherent mechanisms of heterogeneity of reproductive capability...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8411529/ https://www.ncbi.nlm.nih.gov/pubmed/34470660 http://dx.doi.org/10.1186/s40813-021-00226-x |
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author | Ran, Xueqin Hu, Fengbin Mao, Ning Ruan, Yiqi Yi, Fanli Niu, Xi Huang, Shihui Li, Sheng You, Longjiang Zhang, Fuping Tang, Liangting Wang, Jiafu Liu, Jianfeng |
author_facet | Ran, Xueqin Hu, Fengbin Mao, Ning Ruan, Yiqi Yi, Fanli Niu, Xi Huang, Shihui Li, Sheng You, Longjiang Zhang, Fuping Tang, Liangting Wang, Jiafu Liu, Jianfeng |
author_sort | Ran, Xueqin |
collection | PubMed |
description | BACKGROUND: Although lots of quantitative trait loci (QTLs) and genes present roles in litter size of some breeds, the information might not make it clear for the huge diversity of reproductive capability in pig breeds. To elucidate the inherent mechanisms of heterogeneity of reproductive capability in litter size of Xiang pig, we performed transcriptome analysis for the expression profile in ovaries using RNA-seq method. RESULTS: We identified 1,419 up-regulated and 1,376 down-regulated genes in Xiang pigs with large litter size. Among them, 1,010 differentially expressed genes (DEGs) were differently spliced between two groups with large or small litter sizes. Based on GO and KEGG analysis, numerous members of genes were gathered in ovarian steroidogenesis, steroid biosynthesis, oocyte maturation and reproduction processes. CONCLUSIONS: Combined with gene biological function, twelve genes were found out that might be related with the reproductive capability of Xiang pig, of which, eleven genes were recognized as hub genes. These genes may play a role in promoting litter size by elevating steroid and peptide hormones supply through the ovary and facilitating the processes of ovulation and in vivo fertilization. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s40813-021-00226-x. |
format | Online Article Text |
id | pubmed-8411529 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-84115292021-09-09 Differences in gene expression and variable splicing events of ovaries between large and small litter size in Chinese Xiang pigs Ran, Xueqin Hu, Fengbin Mao, Ning Ruan, Yiqi Yi, Fanli Niu, Xi Huang, Shihui Li, Sheng You, Longjiang Zhang, Fuping Tang, Liangting Wang, Jiafu Liu, Jianfeng Porcine Health Manag Research BACKGROUND: Although lots of quantitative trait loci (QTLs) and genes present roles in litter size of some breeds, the information might not make it clear for the huge diversity of reproductive capability in pig breeds. To elucidate the inherent mechanisms of heterogeneity of reproductive capability in litter size of Xiang pig, we performed transcriptome analysis for the expression profile in ovaries using RNA-seq method. RESULTS: We identified 1,419 up-regulated and 1,376 down-regulated genes in Xiang pigs with large litter size. Among them, 1,010 differentially expressed genes (DEGs) were differently spliced between two groups with large or small litter sizes. Based on GO and KEGG analysis, numerous members of genes were gathered in ovarian steroidogenesis, steroid biosynthesis, oocyte maturation and reproduction processes. CONCLUSIONS: Combined with gene biological function, twelve genes were found out that might be related with the reproductive capability of Xiang pig, of which, eleven genes were recognized as hub genes. These genes may play a role in promoting litter size by elevating steroid and peptide hormones supply through the ovary and facilitating the processes of ovulation and in vivo fertilization. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s40813-021-00226-x. BioMed Central 2021-09-01 /pmc/articles/PMC8411529/ /pubmed/34470660 http://dx.doi.org/10.1186/s40813-021-00226-x Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Ran, Xueqin Hu, Fengbin Mao, Ning Ruan, Yiqi Yi, Fanli Niu, Xi Huang, Shihui Li, Sheng You, Longjiang Zhang, Fuping Tang, Liangting Wang, Jiafu Liu, Jianfeng Differences in gene expression and variable splicing events of ovaries between large and small litter size in Chinese Xiang pigs |
title | Differences in gene expression and variable splicing events of ovaries between large and small litter size in Chinese Xiang pigs |
title_full | Differences in gene expression and variable splicing events of ovaries between large and small litter size in Chinese Xiang pigs |
title_fullStr | Differences in gene expression and variable splicing events of ovaries between large and small litter size in Chinese Xiang pigs |
title_full_unstemmed | Differences in gene expression and variable splicing events of ovaries between large and small litter size in Chinese Xiang pigs |
title_short | Differences in gene expression and variable splicing events of ovaries between large and small litter size in Chinese Xiang pigs |
title_sort | differences in gene expression and variable splicing events of ovaries between large and small litter size in chinese xiang pigs |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8411529/ https://www.ncbi.nlm.nih.gov/pubmed/34470660 http://dx.doi.org/10.1186/s40813-021-00226-x |
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