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Multicenter study evaluating novel multi-specimen pooling assay for the detection of SARS-CoV-2: High sensitivity and high throughput testing
BACKGROUND/PURPOSE: Mass screening for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is important to prevent the spread of coronavirus disease 2019 (COVID-19). Pooling samples can increase the number of tests processed. LabTurbo AIO 48 is an automated platform that allows ribonucleic...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taiwan Society of Microbiology. Published by Elsevier Taiwan LLC.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8411585/ https://www.ncbi.nlm.nih.gov/pubmed/34538568 http://dx.doi.org/10.1016/j.jmii.2021.08.003 |
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author | Chung, Hsing-Yi Jian, Ming-Jr Chang, Chih-Kai Lin, Jung-Chung Yeh, Kuo-Ming Chen, Chien-Wen Yang, Ya-Sung Hsieh, Shan-Shan Chen, En-Sung Yang, Mei-Hsiu Tang, Sheng-Hui Perng, Cherng-Lih Yang, Ji-Rong Liu, Ming-Tsan Chang, Feng-Yee Shang, Hung-Sheng |
author_facet | Chung, Hsing-Yi Jian, Ming-Jr Chang, Chih-Kai Lin, Jung-Chung Yeh, Kuo-Ming Chen, Chien-Wen Yang, Ya-Sung Hsieh, Shan-Shan Chen, En-Sung Yang, Mei-Hsiu Tang, Sheng-Hui Perng, Cherng-Lih Yang, Ji-Rong Liu, Ming-Tsan Chang, Feng-Yee Shang, Hung-Sheng |
author_sort | Chung, Hsing-Yi |
collection | PubMed |
description | BACKGROUND/PURPOSE: Mass screening for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is important to prevent the spread of coronavirus disease 2019 (COVID-19). Pooling samples can increase the number of tests processed. LabTurbo AIO 48 is an automated platform that allows ribonucleic acid extraction and sample analysis on the same instrument. We created a novel pooling assay on this platform for SARS-CoV-2 detection and demonstrated that the pooling strategy increases testing capacity without affecting accuracy and sensitivity. METHODS: Comparative limit of detection (LoD) assessment was performed on the LabTurbo AIO 48 platform and the current standard detection system based on real-time reverse transcription polymerase chain reaction (rRT-PCR) using 55 clinically positive samples. An additional 330 primary clinical samples were assessed. RESULTS: Six samples pooled into one reaction tube were detected in approximately 2.5 h using the World Health Organization rRT-PCR protocol. LabTurbo AIO 48 also demonstrated a higher throughput than our reference rRT-PCR assay, with an LoD of 1000 copies/mL. The overall percentage agreement between the methods for the 330 samples was 100%. CONCLUSION: We created a novel multi-specimen pooling assay using LabTurbo AIO 48 for the robust detection of SARS-CoV-2, allowing high-throughput results; this assay will aid in better control and prevention of COVID-19. The diagnostic assay was cost-effective and time-efficient; thus, the pooling strategy is a practical and effective method for diagnosing large quantities of specimens without compromising precision. |
format | Online Article Text |
id | pubmed-8411585 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Taiwan Society of Microbiology. Published by Elsevier Taiwan LLC. |
record_format | MEDLINE/PubMed |
spelling | pubmed-84115852021-09-03 Multicenter study evaluating novel multi-specimen pooling assay for the detection of SARS-CoV-2: High sensitivity and high throughput testing Chung, Hsing-Yi Jian, Ming-Jr Chang, Chih-Kai Lin, Jung-Chung Yeh, Kuo-Ming Chen, Chien-Wen Yang, Ya-Sung Hsieh, Shan-Shan Chen, En-Sung Yang, Mei-Hsiu Tang, Sheng-Hui Perng, Cherng-Lih Yang, Ji-Rong Liu, Ming-Tsan Chang, Feng-Yee Shang, Hung-Sheng J Microbiol Immunol Infect Original Article BACKGROUND/PURPOSE: Mass screening for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is important to prevent the spread of coronavirus disease 2019 (COVID-19). Pooling samples can increase the number of tests processed. LabTurbo AIO 48 is an automated platform that allows ribonucleic acid extraction and sample analysis on the same instrument. We created a novel pooling assay on this platform for SARS-CoV-2 detection and demonstrated that the pooling strategy increases testing capacity without affecting accuracy and sensitivity. METHODS: Comparative limit of detection (LoD) assessment was performed on the LabTurbo AIO 48 platform and the current standard detection system based on real-time reverse transcription polymerase chain reaction (rRT-PCR) using 55 clinically positive samples. An additional 330 primary clinical samples were assessed. RESULTS: Six samples pooled into one reaction tube were detected in approximately 2.5 h using the World Health Organization rRT-PCR protocol. LabTurbo AIO 48 also demonstrated a higher throughput than our reference rRT-PCR assay, with an LoD of 1000 copies/mL. The overall percentage agreement between the methods for the 330 samples was 100%. CONCLUSION: We created a novel multi-specimen pooling assay using LabTurbo AIO 48 for the robust detection of SARS-CoV-2, allowing high-throughput results; this assay will aid in better control and prevention of COVID-19. The diagnostic assay was cost-effective and time-efficient; thus, the pooling strategy is a practical and effective method for diagnosing large quantities of specimens without compromising precision. Taiwan Society of Microbiology. Published by Elsevier Taiwan LLC. 2022-12 2021-09-02 /pmc/articles/PMC8411585/ /pubmed/34538568 http://dx.doi.org/10.1016/j.jmii.2021.08.003 Text en © 2021 Taiwan Society of Microbiology. Published by Elsevier Taiwan LLC. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Original Article Chung, Hsing-Yi Jian, Ming-Jr Chang, Chih-Kai Lin, Jung-Chung Yeh, Kuo-Ming Chen, Chien-Wen Yang, Ya-Sung Hsieh, Shan-Shan Chen, En-Sung Yang, Mei-Hsiu Tang, Sheng-Hui Perng, Cherng-Lih Yang, Ji-Rong Liu, Ming-Tsan Chang, Feng-Yee Shang, Hung-Sheng Multicenter study evaluating novel multi-specimen pooling assay for the detection of SARS-CoV-2: High sensitivity and high throughput testing |
title | Multicenter study evaluating novel multi-specimen pooling assay for the detection of SARS-CoV-2: High sensitivity and high throughput testing |
title_full | Multicenter study evaluating novel multi-specimen pooling assay for the detection of SARS-CoV-2: High sensitivity and high throughput testing |
title_fullStr | Multicenter study evaluating novel multi-specimen pooling assay for the detection of SARS-CoV-2: High sensitivity and high throughput testing |
title_full_unstemmed | Multicenter study evaluating novel multi-specimen pooling assay for the detection of SARS-CoV-2: High sensitivity and high throughput testing |
title_short | Multicenter study evaluating novel multi-specimen pooling assay for the detection of SARS-CoV-2: High sensitivity and high throughput testing |
title_sort | multicenter study evaluating novel multi-specimen pooling assay for the detection of sars-cov-2: high sensitivity and high throughput testing |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8411585/ https://www.ncbi.nlm.nih.gov/pubmed/34538568 http://dx.doi.org/10.1016/j.jmii.2021.08.003 |
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