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Site 2 of the Yersinia pestis substrate-binding protein YfeA is a dynamic surface metal-binding site

The substrate-binding protein YfeA (also known as YPO2439 or y1897) is a polyspecific metal-binding protein that is crucial for nutrient acquisition and virulence in Yersinia pestis, the causative microbe of plague. YfeA folds into a monomeric c-clamp like other substrate-binding proteins and has tw...

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Autores principales: Radka, Christopher D., Aller, Stephen G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8411934/
https://www.ncbi.nlm.nih.gov/pubmed/34473105
http://dx.doi.org/10.1107/S2053230X21008086
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author Radka, Christopher D.
Aller, Stephen G.
author_facet Radka, Christopher D.
Aller, Stephen G.
author_sort Radka, Christopher D.
collection PubMed
description The substrate-binding protein YfeA (also known as YPO2439 or y1897) is a polyspecific metal-binding protein that is crucial for nutrient acquisition and virulence in Yersinia pestis, the causative microbe of plague. YfeA folds into a monomeric c-clamp like other substrate-binding proteins and has two metal-binding sites (sites 1 and 2). Site 2 is a bidentate surface site capable of binding Zn and Mn atoms and is a unique feature of YfeA. Occasionally, the site 2 residues of two YfeA molecules will cooperate with the histidine tag of a third YfeA molecule in coordinating the same metal and lead to metal-dependent crystallographic packing. Here, three crystal structures of YfeA are presented at 1.85, 2.05 and 2.25 Å resolution. A comparison of the structures reveals that the metal can be displaced at five different locations ranging from ∼4 to ∼16 Å away from the canonical site 2. These observations reveal different configurations of site 2 that enable cooperative metal binding and demonstrate how site 2 is dynamic and freely available for inter-protein metal coordination.
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spelling pubmed-84119342021-09-13 Site 2 of the Yersinia pestis substrate-binding protein YfeA is a dynamic surface metal-binding site Radka, Christopher D. Aller, Stephen G. Acta Crystallogr F Struct Biol Commun Research Communications The substrate-binding protein YfeA (also known as YPO2439 or y1897) is a polyspecific metal-binding protein that is crucial for nutrient acquisition and virulence in Yersinia pestis, the causative microbe of plague. YfeA folds into a monomeric c-clamp like other substrate-binding proteins and has two metal-binding sites (sites 1 and 2). Site 2 is a bidentate surface site capable of binding Zn and Mn atoms and is a unique feature of YfeA. Occasionally, the site 2 residues of two YfeA molecules will cooperate with the histidine tag of a third YfeA molecule in coordinating the same metal and lead to metal-dependent crystallographic packing. Here, three crystal structures of YfeA are presented at 1.85, 2.05 and 2.25 Å resolution. A comparison of the structures reveals that the metal can be displaced at five different locations ranging from ∼4 to ∼16 Å away from the canonical site 2. These observations reveal different configurations of site 2 that enable cooperative metal binding and demonstrate how site 2 is dynamic and freely available for inter-protein metal coordination. International Union of Crystallography 2021-08-24 /pmc/articles/PMC8411934/ /pubmed/34473105 http://dx.doi.org/10.1107/S2053230X21008086 Text en © Radka and Aller 2021 https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.
spellingShingle Research Communications
Radka, Christopher D.
Aller, Stephen G.
Site 2 of the Yersinia pestis substrate-binding protein YfeA is a dynamic surface metal-binding site
title Site 2 of the Yersinia pestis substrate-binding protein YfeA is a dynamic surface metal-binding site
title_full Site 2 of the Yersinia pestis substrate-binding protein YfeA is a dynamic surface metal-binding site
title_fullStr Site 2 of the Yersinia pestis substrate-binding protein YfeA is a dynamic surface metal-binding site
title_full_unstemmed Site 2 of the Yersinia pestis substrate-binding protein YfeA is a dynamic surface metal-binding site
title_short Site 2 of the Yersinia pestis substrate-binding protein YfeA is a dynamic surface metal-binding site
title_sort site 2 of the yersinia pestis substrate-binding protein yfea is a dynamic surface metal-binding site
topic Research Communications
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8411934/
https://www.ncbi.nlm.nih.gov/pubmed/34473105
http://dx.doi.org/10.1107/S2053230X21008086
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