Determination of Advantages and Limitations of qPCR Duplexing in a Single Fluorescent Channel

[Image: see text] Real-time (quantitative) polymerase chain reaction (qPCR) has been widely applied in molecular diagnostics due to its immense sensitivity and specificity. qPCR multiplexing, based either on fluorescent probes or intercalating dyes, greatly expanded PCR capability due to the concurr...

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Detalles Bibliográficos
Autores principales: Zhang, Haoqing, Yan, Zhiqiang, Wang, Xinlu, Gaňová, Martina, Chang, Honglong, Laššáková, Soňa, Korabecna, Marie, Neuzil, Pavel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2021
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8412922/
https://www.ncbi.nlm.nih.gov/pubmed/34497918
http://dx.doi.org/10.1021/acsomega.1c02971

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8412922/
https://www.ncbi.nlm.nih.gov/pubmed/34497918
http://dx.doi.org/10.1021/acsomega.1c02971

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