Ultra-fast, high throughput and inexpensive detection of SARS-CoV-2 seroconversion using Ni(2+) magnetic beads

To monitor the levels of protecting antibodies raised in the population in response to infection and/or to immunization with SARS-CoV-2, we need a technique that allows high throughput and low-cost quantitative analysis of human IgG antibodies reactive against viral antigens. Here we describe an ult...

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Detalles Bibliográficos
Autores principales: Conzentino, Marcelo S., Santos, Tatielle P.C., Selim, Khaled A., Wagner, Berenike, Alford, Janette T., Deobald, Nelli, Paula, Nigela M., Rego, Fabiane G.M., Zanette, Dalila L., Aoki, Mateus N., Nardin, Jeanine M., Huergo, Maria C.C., Reis, Rodrigo A., Huergo, Luciano F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Inc. 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8413102/
https://www.ncbi.nlm.nih.gov/pubmed/34481802
http://dx.doi.org/10.1016/j.ab.2021.114360
Descripción
Sumario:To monitor the levels of protecting antibodies raised in the population in response to infection and/or to immunization with SARS-CoV-2, we need a technique that allows high throughput and low-cost quantitative analysis of human IgG antibodies reactive against viral antigens. Here we describe an ultra-fast, high throughput and inexpensive assay to detect SARS-CoV-2 seroconversion in humans. The assay is based on Ni(2+) magnetic particles coated with His tagged SARS-CoV-2 antigens. A simple and inexpensive 96 well plate magnetic extraction/homogenization process is described which allows the simultaneous analysis of 96 samples and delivers results in 7 min with high accuracy.

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