Ultra-fast, high throughput and inexpensive detection of SARS-CoV-2 seroconversion using Ni(2+) magnetic beads

To monitor the levels of protecting antibodies raised in the population in response to infection and/or to immunization with SARS-CoV-2, we need a technique that allows high throughput and low-cost quantitative analysis of human IgG antibodies reactive against viral antigens. Here we describe an ult...

Descripción completa

Detalles Bibliográficos
Autores principales: Conzentino, Marcelo S., Santos, Tatielle P.C., Selim, Khaled A., Wagner, Berenike, Alford, Janette T., Deobald, Nelli, Paula, Nigela M., Rego, Fabiane G.M., Zanette, Dalila L., Aoki, Mateus N., Nardin, Jeanine M., Huergo, Maria C.C., Reis, Rodrigo A., Huergo, Luciano F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Inc. 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8413102/
https://www.ncbi.nlm.nih.gov/pubmed/34481802
http://dx.doi.org/10.1016/j.ab.2021.114360
_version_ 1783747590997671936
author Conzentino, Marcelo S.
Santos, Tatielle P.C.
Selim, Khaled A.
Wagner, Berenike
Alford, Janette T.
Deobald, Nelli
Paula, Nigela M.
Rego, Fabiane G.M.
Zanette, Dalila L.
Aoki, Mateus N.
Nardin, Jeanine M.
Huergo, Maria C.C.
Reis, Rodrigo A.
Huergo, Luciano F.
author_facet Conzentino, Marcelo S.
Santos, Tatielle P.C.
Selim, Khaled A.
Wagner, Berenike
Alford, Janette T.
Deobald, Nelli
Paula, Nigela M.
Rego, Fabiane G.M.
Zanette, Dalila L.
Aoki, Mateus N.
Nardin, Jeanine M.
Huergo, Maria C.C.
Reis, Rodrigo A.
Huergo, Luciano F.
author_sort Conzentino, Marcelo S.
collection PubMed
description To monitor the levels of protecting antibodies raised in the population in response to infection and/or to immunization with SARS-CoV-2, we need a technique that allows high throughput and low-cost quantitative analysis of human IgG antibodies reactive against viral antigens. Here we describe an ultra-fast, high throughput and inexpensive assay to detect SARS-CoV-2 seroconversion in humans. The assay is based on Ni(2+) magnetic particles coated with His tagged SARS-CoV-2 antigens. A simple and inexpensive 96 well plate magnetic extraction/homogenization process is described which allows the simultaneous analysis of 96 samples and delivers results in 7 min with high accuracy.
format Online
Article
Text
id pubmed-8413102
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Elsevier Inc.
record_format MEDLINE/PubMed
spelling pubmed-84131022021-09-03 Ultra-fast, high throughput and inexpensive detection of SARS-CoV-2 seroconversion using Ni(2+) magnetic beads Conzentino, Marcelo S. Santos, Tatielle P.C. Selim, Khaled A. Wagner, Berenike Alford, Janette T. Deobald, Nelli Paula, Nigela M. Rego, Fabiane G.M. Zanette, Dalila L. Aoki, Mateus N. Nardin, Jeanine M. Huergo, Maria C.C. Reis, Rodrigo A. Huergo, Luciano F. Anal Biochem Article To monitor the levels of protecting antibodies raised in the population in response to infection and/or to immunization with SARS-CoV-2, we need a technique that allows high throughput and low-cost quantitative analysis of human IgG antibodies reactive against viral antigens. Here we describe an ultra-fast, high throughput and inexpensive assay to detect SARS-CoV-2 seroconversion in humans. The assay is based on Ni(2+) magnetic particles coated with His tagged SARS-CoV-2 antigens. A simple and inexpensive 96 well plate magnetic extraction/homogenization process is described which allows the simultaneous analysis of 96 samples and delivers results in 7 min with high accuracy. Elsevier Inc. 2021-10-15 2021-09-03 /pmc/articles/PMC8413102/ /pubmed/34481802 http://dx.doi.org/10.1016/j.ab.2021.114360 Text en © 2021 Elsevier Inc. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Conzentino, Marcelo S.
Santos, Tatielle P.C.
Selim, Khaled A.
Wagner, Berenike
Alford, Janette T.
Deobald, Nelli
Paula, Nigela M.
Rego, Fabiane G.M.
Zanette, Dalila L.
Aoki, Mateus N.
Nardin, Jeanine M.
Huergo, Maria C.C.
Reis, Rodrigo A.
Huergo, Luciano F.
Ultra-fast, high throughput and inexpensive detection of SARS-CoV-2 seroconversion using Ni(2+) magnetic beads
title Ultra-fast, high throughput and inexpensive detection of SARS-CoV-2 seroconversion using Ni(2+) magnetic beads
title_full Ultra-fast, high throughput and inexpensive detection of SARS-CoV-2 seroconversion using Ni(2+) magnetic beads
title_fullStr Ultra-fast, high throughput and inexpensive detection of SARS-CoV-2 seroconversion using Ni(2+) magnetic beads
title_full_unstemmed Ultra-fast, high throughput and inexpensive detection of SARS-CoV-2 seroconversion using Ni(2+) magnetic beads
title_short Ultra-fast, high throughput and inexpensive detection of SARS-CoV-2 seroconversion using Ni(2+) magnetic beads
title_sort ultra-fast, high throughput and inexpensive detection of sars-cov-2 seroconversion using ni(2+) magnetic beads
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8413102/
https://www.ncbi.nlm.nih.gov/pubmed/34481802
http://dx.doi.org/10.1016/j.ab.2021.114360
work_keys_str_mv AT conzentinomarcelos ultrafasthighthroughputandinexpensivedetectionofsarscov2seroconversionusingni2magneticbeads
AT santostatiellepc ultrafasthighthroughputandinexpensivedetectionofsarscov2seroconversionusingni2magneticbeads
AT selimkhaleda ultrafasthighthroughputandinexpensivedetectionofsarscov2seroconversionusingni2magneticbeads
AT wagnerberenike ultrafasthighthroughputandinexpensivedetectionofsarscov2seroconversionusingni2magneticbeads
AT alfordjanettet ultrafasthighthroughputandinexpensivedetectionofsarscov2seroconversionusingni2magneticbeads
AT deobaldnelli ultrafasthighthroughputandinexpensivedetectionofsarscov2seroconversionusingni2magneticbeads
AT paulanigelam ultrafasthighthroughputandinexpensivedetectionofsarscov2seroconversionusingni2magneticbeads
AT regofabianegm ultrafasthighthroughputandinexpensivedetectionofsarscov2seroconversionusingni2magneticbeads
AT zanettedalilal ultrafasthighthroughputandinexpensivedetectionofsarscov2seroconversionusingni2magneticbeads
AT aokimateusn ultrafasthighthroughputandinexpensivedetectionofsarscov2seroconversionusingni2magneticbeads
AT nardinjeaninem ultrafasthighthroughputandinexpensivedetectionofsarscov2seroconversionusingni2magneticbeads
AT huergomariacc ultrafasthighthroughputandinexpensivedetectionofsarscov2seroconversionusingni2magneticbeads
AT reisrodrigoa ultrafasthighthroughputandinexpensivedetectionofsarscov2seroconversionusingni2magneticbeads
AT huergolucianof ultrafasthighthroughputandinexpensivedetectionofsarscov2seroconversionusingni2magneticbeads

Ejemplares similares