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Antiviral and virucidal activities of lycorine on duck tembusu virus in vitro by blocking viral internalization and entry

Duck tembusu virus (DTMUV) was firstly identified in 2010 in China; since then, it has caused enormous economic loss to breeding industry. Great efforts have been made to develop drugs and vaccines against DTMUV. However, current available vaccines or anti-DTMUV drugs are consistently inefficient. H...

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Autores principales: Lv, Xuan, Zhang, Miao, Yu, Shengzhu, Zhang, Chong, Fang, Tian, Liu, Dan, Jia, Beiping, Zhu, Meng, Wang, Bei, Wang, Qing, Zhu, Yingqi, Wang, Guijun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8414183/
https://www.ncbi.nlm.nih.gov/pubmed/34478911
http://dx.doi.org/10.1016/j.psj.2021.101404
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author Lv, Xuan
Zhang, Miao
Yu, Shengzhu
Zhang, Chong
Fang, Tian
Liu, Dan
Jia, Beiping
Zhu, Meng
Wang, Bei
Wang, Qing
Zhu, Yingqi
Wang, Guijun
author_facet Lv, Xuan
Zhang, Miao
Yu, Shengzhu
Zhang, Chong
Fang, Tian
Liu, Dan
Jia, Beiping
Zhu, Meng
Wang, Bei
Wang, Qing
Zhu, Yingqi
Wang, Guijun
author_sort Lv, Xuan
collection PubMed
description Duck tembusu virus (DTMUV) was firstly identified in 2010 in China; since then, it has caused enormous economic loss to breeding industry. Great efforts have been made to develop drugs and vaccines against DTMUV. However, current available vaccines or anti-DTMUV drugs are consistently inefficient. Hence, various more broadly effective drugs have become important for the treatment of DTMUV infection; among these, lycorine, one of the important sources of active alkaloids, is a promising example. Nevertheless, it is not known whether lycorine has any antiviral activities against DTMUV. Therefore, the purpose of the present study is to investigate the anti-DTMUV abilities of lycorine. The cytotoxicity of lycorine was evaluated on BHK-21 cells by CCK-8 assay, and its antiviral effect against DTMUV was examined by real-time PCR assays, virus titer determination, Western blot and immunofluorescence (IFA) assays, respectively. Furthermore, the underlying mechanisms of the anti-DTMUV effects of lycorine were also investigated. The results indicated that the highest nontoxicity concentration of lycorine on BHK-21 cells was 5 µM. Lycorine possessed the antiviral ability against DTMUV on BHK-21 cells, as demonstrated by the reduction of virus titers and copy numbers in vitro. Western blot and IFA analysis showed the inhibitory effect of lycorine on DTMUV envelope (E) protein expression. Moreover, using time-of-addition assays, we found that lycorine displays its antivirus and virucidal activities through blocking viral internalization and entry in vitro. Taken together, our findings firstly demonstrate the antiviral activities of lycorine against DTMUV, suggesting that lycorine can be a potential drug for the treatment of DTMUV infection.
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spelling pubmed-84141832021-09-08 Antiviral and virucidal activities of lycorine on duck tembusu virus in vitro by blocking viral internalization and entry Lv, Xuan Zhang, Miao Yu, Shengzhu Zhang, Chong Fang, Tian Liu, Dan Jia, Beiping Zhu, Meng Wang, Bei Wang, Qing Zhu, Yingqi Wang, Guijun Poult Sci IMMUNOLOGY, HEALTH AND DISEASE Duck tembusu virus (DTMUV) was firstly identified in 2010 in China; since then, it has caused enormous economic loss to breeding industry. Great efforts have been made to develop drugs and vaccines against DTMUV. However, current available vaccines or anti-DTMUV drugs are consistently inefficient. Hence, various more broadly effective drugs have become important for the treatment of DTMUV infection; among these, lycorine, one of the important sources of active alkaloids, is a promising example. Nevertheless, it is not known whether lycorine has any antiviral activities against DTMUV. Therefore, the purpose of the present study is to investigate the anti-DTMUV abilities of lycorine. The cytotoxicity of lycorine was evaluated on BHK-21 cells by CCK-8 assay, and its antiviral effect against DTMUV was examined by real-time PCR assays, virus titer determination, Western blot and immunofluorescence (IFA) assays, respectively. Furthermore, the underlying mechanisms of the anti-DTMUV effects of lycorine were also investigated. The results indicated that the highest nontoxicity concentration of lycorine on BHK-21 cells was 5 µM. Lycorine possessed the antiviral ability against DTMUV on BHK-21 cells, as demonstrated by the reduction of virus titers and copy numbers in vitro. Western blot and IFA analysis showed the inhibitory effect of lycorine on DTMUV envelope (E) protein expression. Moreover, using time-of-addition assays, we found that lycorine displays its antivirus and virucidal activities through blocking viral internalization and entry in vitro. Taken together, our findings firstly demonstrate the antiviral activities of lycorine against DTMUV, suggesting that lycorine can be a potential drug for the treatment of DTMUV infection. Elsevier 2021-07-25 /pmc/articles/PMC8414183/ /pubmed/34478911 http://dx.doi.org/10.1016/j.psj.2021.101404 Text en © 2021 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle IMMUNOLOGY, HEALTH AND DISEASE
Lv, Xuan
Zhang, Miao
Yu, Shengzhu
Zhang, Chong
Fang, Tian
Liu, Dan
Jia, Beiping
Zhu, Meng
Wang, Bei
Wang, Qing
Zhu, Yingqi
Wang, Guijun
Antiviral and virucidal activities of lycorine on duck tembusu virus in vitro by blocking viral internalization and entry
title Antiviral and virucidal activities of lycorine on duck tembusu virus in vitro by blocking viral internalization and entry
title_full Antiviral and virucidal activities of lycorine on duck tembusu virus in vitro by blocking viral internalization and entry
title_fullStr Antiviral and virucidal activities of lycorine on duck tembusu virus in vitro by blocking viral internalization and entry
title_full_unstemmed Antiviral and virucidal activities of lycorine on duck tembusu virus in vitro by blocking viral internalization and entry
title_short Antiviral and virucidal activities of lycorine on duck tembusu virus in vitro by blocking viral internalization and entry
title_sort antiviral and virucidal activities of lycorine on duck tembusu virus in vitro by blocking viral internalization and entry
topic IMMUNOLOGY, HEALTH AND DISEASE
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8414183/
https://www.ncbi.nlm.nih.gov/pubmed/34478911
http://dx.doi.org/10.1016/j.psj.2021.101404
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