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Phylotaxogenomics for the Reappraisal of the Genus Roseomonas With the Creation of Six New Genera

The genus Roseomonas is a significant group of bacteria which is invariably of great clinical and ecological importance. Previous studies have shown that the genus Roseomonas is polyphyletic in nature. Our present study focused on generating a lucid understanding of the phylogenetic framework for th...

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Autores principales: Rai, Anusha, Jagadeeshwari, Uppada, Deepshikha, Gupta, Smita, Nandardhane, Sasikala, Chintalapati, Ramana, Chintalapati Venkata
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8414978/
https://www.ncbi.nlm.nih.gov/pubmed/34484138
http://dx.doi.org/10.3389/fmicb.2021.677842
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author Rai, Anusha
Jagadeeshwari, Uppada
Deepshikha, Gupta
Smita, Nandardhane
Sasikala, Chintalapati
Ramana, Chintalapati Venkata
author_facet Rai, Anusha
Jagadeeshwari, Uppada
Deepshikha, Gupta
Smita, Nandardhane
Sasikala, Chintalapati
Ramana, Chintalapati Venkata
author_sort Rai, Anusha
collection PubMed
description The genus Roseomonas is a significant group of bacteria which is invariably of great clinical and ecological importance. Previous studies have shown that the genus Roseomonas is polyphyletic in nature. Our present study focused on generating a lucid understanding of the phylogenetic framework for the re-evaluation and reclassification of the genus Roseomonas. Phylogenetic studies based on the 16S rRNA gene and 92 concatenated genes suggested that the genus is heterogeneous, forming seven major groups. Existing Roseomonas species were subjected to an array of genomic, phenotypic, and chemotaxonomic analyses in order to resolve the heterogeneity. Genomic similarity indices (dDDH and ANI) indicated that the members were well-defined at the species level. The Percentage of Conserved Proteins (POCP) and the average Amino Acid Identity (AAI) values between the groups of the genus Roseomonas and other interspersing members of the family Acetobacteraceae were below 65 and 70%, respectively. The pan-genome evaluation depicted that the pan-genome was an open type and the members shared 958 core genes. This claim of reclassification was equally supported by the phenotypic and chemotaxonomic differences between the groups. Thus, in this study, we propose to re-evaluate and reclassify the genus Roseomonas and propose six novel genera as Pararoseomonas gen. nov., Falsiroseomonas gen. nov., Paeniroseomonas gen. nov., Plastoroseomonas gen. nov., Neoroseomonas gen. nov., and Pseudoroseomonas gen. nov.
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spelling pubmed-84149782021-09-04 Phylotaxogenomics for the Reappraisal of the Genus Roseomonas With the Creation of Six New Genera Rai, Anusha Jagadeeshwari, Uppada Deepshikha, Gupta Smita, Nandardhane Sasikala, Chintalapati Ramana, Chintalapati Venkata Front Microbiol Microbiology The genus Roseomonas is a significant group of bacteria which is invariably of great clinical and ecological importance. Previous studies have shown that the genus Roseomonas is polyphyletic in nature. Our present study focused on generating a lucid understanding of the phylogenetic framework for the re-evaluation and reclassification of the genus Roseomonas. Phylogenetic studies based on the 16S rRNA gene and 92 concatenated genes suggested that the genus is heterogeneous, forming seven major groups. Existing Roseomonas species were subjected to an array of genomic, phenotypic, and chemotaxonomic analyses in order to resolve the heterogeneity. Genomic similarity indices (dDDH and ANI) indicated that the members were well-defined at the species level. The Percentage of Conserved Proteins (POCP) and the average Amino Acid Identity (AAI) values between the groups of the genus Roseomonas and other interspersing members of the family Acetobacteraceae were below 65 and 70%, respectively. The pan-genome evaluation depicted that the pan-genome was an open type and the members shared 958 core genes. This claim of reclassification was equally supported by the phenotypic and chemotaxonomic differences between the groups. Thus, in this study, we propose to re-evaluate and reclassify the genus Roseomonas and propose six novel genera as Pararoseomonas gen. nov., Falsiroseomonas gen. nov., Paeniroseomonas gen. nov., Plastoroseomonas gen. nov., Neoroseomonas gen. nov., and Pseudoroseomonas gen. nov. Frontiers Media S.A. 2021-08-13 /pmc/articles/PMC8414978/ /pubmed/34484138 http://dx.doi.org/10.3389/fmicb.2021.677842 Text en Copyright © 2021 Rai, Jagadeeshwari, Deepshikha, Smita, Sasikala and Ramana. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Rai, Anusha
Jagadeeshwari, Uppada
Deepshikha, Gupta
Smita, Nandardhane
Sasikala, Chintalapati
Ramana, Chintalapati Venkata
Phylotaxogenomics for the Reappraisal of the Genus Roseomonas With the Creation of Six New Genera
title Phylotaxogenomics for the Reappraisal of the Genus Roseomonas With the Creation of Six New Genera
title_full Phylotaxogenomics for the Reappraisal of the Genus Roseomonas With the Creation of Six New Genera
title_fullStr Phylotaxogenomics for the Reappraisal of the Genus Roseomonas With the Creation of Six New Genera
title_full_unstemmed Phylotaxogenomics for the Reappraisal of the Genus Roseomonas With the Creation of Six New Genera
title_short Phylotaxogenomics for the Reappraisal of the Genus Roseomonas With the Creation of Six New Genera
title_sort phylotaxogenomics for the reappraisal of the genus roseomonas with the creation of six new genera
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8414978/
https://www.ncbi.nlm.nih.gov/pubmed/34484138
http://dx.doi.org/10.3389/fmicb.2021.677842
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