Cargando…

Cell-Free Glycoengineering of the Recombinant SARS-CoV-2 Spike Glycoprotein

The baculovirus-insect cell expression system is readily utilized to produce viral glycoproteins for research as well as for subunit vaccines and vaccine candidates, for instance against SARS-CoV-2 infections. However, the glycoforms of recombinant proteins derived from this expression system are in...

Descripción completa

Detalles Bibliográficos
Autores principales:	Ruhnau, Johannes, Grote, Valerian, Juarez-Osorio, Mariana, Bruder, Dunja, Mahour, Reza, Rapp, Erdmann, Rexer, Thomas F. T., Reichl, Udo
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Frontiers Media S.A. 2021
Materias:	Bioengineering and Biotechnology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8415157/ https://www.ncbi.nlm.nih.gov/pubmed/34485255 http://dx.doi.org/10.3389/fbioe.2021.699025

Ejemplares similares

One pot synthesis of GDP‐mannose by a multi‐enzyme cascade for enzymatic assembly of lipid‐linked oligosaccharides
por: Rexer, Thomas F.T., et al.
Publicado: (2017)

Cell‐Free Multi‐Enzyme Synthesis and Purification of Uridine Diphosphate Galactose
por: Mahour, Reza, et al.
Publicado: (2021)

Metabolic Glycoengineering Enables the Ultrastructural Visualization of Sialic Acids in the Glycocalyx of the Alveolar Epithelial Cell Line hAELVi
por: Brandt, Raphael, et al.
Publicado: (2021)

Synthetische Glykobiotechnologie
por: Rexer, Thomas, et al.
Publicado: (2021)

Application of an Inclined Settler for Cell Culture-Based Influenza A Virus Production in Perfusion Mode
por: Coronel, Juliana, et al.
Publicado: (2020)

Production of Defective Interfering Particles of Influenza A Virus in Parallel Continuous Cultures at Two Residence Times—Insights From qPCR Measurements and Viral Dynamics Modeling
por: Tapia, Felipe, et al.
Publicado: (2019)

Cell-free N-glycosylation of peptides using synthetic lipid-linked hybrid and complex N-glycans
por: Wenzel, Lisa, et al.
Publicado: (2023)

Enzymatic Cascades for Tailored (13)C(6) and (15)N Enriched Human Milk Oligosaccharides
por: Fischöder, Thomas, et al.
Publicado: (2019)

MDCK and Vero cells for influenza virus vaccine production: a one-to-one comparison up to lab-scale bioreactor cultivation
por: Genzel, Yvonne, et al.
Publicado: (2010)

Cytoplasmic glycoengineering enables biosynthesis of nanoscale glycoprotein assemblies
por: Tytgat, Hanne L. P., et al.
Publicado: (2019)

An Engineered Pathway for Production of Terminally Sialylated N-glycoproteins in the Periplasm of Escherichia coli
por: Zhu, Jing, et al.
Publicado: (2020)

Production of Melanins With Recombinant Microorganisms
por: Martínez, Luz María, et al.
Publicado: (2019)

Production of Vesicular Stomatitis Virus Glycoprotein-Pseudotyped Lentiviral Vector Is Enhanced by Ezrin Silencing
por: Izumida, Mai, et al.
Publicado: (2020)

Targeted Codelivery of Prodigiosin and Simvastatin Using Smart BioMOF: Functionalization by Recombinant Anti-VEGFR1 scFv
por: Mirzaeinia, Somayyeh, et al.
Publicado: (2022)

Cost of Manufacturing for Recombinant Snakebite Antivenoms
por: Jenkins, Timothy Patrick, et al.
Publicado: (2020)

Recombinant Spider Silk: Promises and Bottlenecks
por: Ramezaniaghdam, Maryam, et al.
Publicado: (2022)

DNA self-assembly nanoflower reverse P-glycoprotein mediated drug resistance in chronic myelogenous leukemia therapy
por: Zhao, Pengxuan, et al.
Publicado: (2023)

The Promoter Toolbox for Recombinant Gene Expression in Trichoderma reesei
por: Fitz, Elisabeth, et al.
Publicado: (2018)

Treatment of Acute Wounds With Recombinant Human-Like Collagen and Recombinant Human-Like Fibronectin in C57BL/6 Mice Individually or in Combination
por: Dong, Yunqing, et al.
Publicado: (2022)

Pulse Shape and Timing Dependence on the Spike-Timing Dependent Plasticity Response of Ion-Conducting Memristors as Synapses
por: Campbell, Kristy A., et al.
Publicado: (2016)

Advances in Using Hansenula polymorpha as Chassis for Recombinant Protein Production
por: Manfrão-Netto, João Heitor Colombelli, et al.
Publicado: (2019)

Editorial: New Technologies for Women’s Health
por: McClements, Lana, et al.
Publicado: (2022)

Fermentative Production of N-Methylglutamate From Glycerol by Recombinant Pseudomonas putida
por: Mindt, Melanie, et al.
Publicado: (2018)

High-Level Production of Recombinant Snowdrop Lectin in Sugarcane and Energy Cane
por: Padilla, Carmen S., et al.
Publicado: (2020)

Downstream Processing of Chlamydomonas reinhardtii TN72 for Recombinant Protein Recovery
por: Stoffels, Laura, et al.
Publicado: (2019)

Recent Developments in Bioprocessing of Recombinant Proteins: Expression Hosts and Process Development
por: Tripathi, Nagesh K., et al.
Publicado: (2019)

Evaluation of Microalgae as Immunostimulants and Recombinant Vaccines for Diseases Prevention and Control in Aquaculture
por: Ma, Ke, et al.
Publicado: (2020)

Comparative Economic Analysis Between Endogenous and Recombinant Production of Hyaluronic Acid
por: Torres-Acosta, Mario A., et al.
Publicado: (2021)

Host-Dependent Producibility of Recombinant Cypridina noctiluca Luciferase With Glycosylation Defects
por: Mitani, Yasuo, et al.
Publicado: (2022)

Strategies to Enhance Periplasmic Recombinant Protein Production Yields in Escherichia coli
por: Karyolaimos, Alexandros, et al.
Publicado: (2021)

Tendon mechanical properties are enhanced via recombinant lysyl oxidase treatment
por: Nguyen, Phong K., et al.
Publicado: (2022)

Optimizing CRE and PhiC31 mediated recombination in Aedes aegypti
por: Carabajal Paladino, Leonela Z., et al.
Publicado: (2023)

Perspectives on Assembling Coronavirus Spikes on Fiber Optics to Reveal Broadly Recognizing Antibodies and Generate a Universal Coronavirus Detector
por: Sypabekova, Marzhan, et al.
Publicado: (2021)

Serum-Free Medium for Recombinant Protein Expression in Chinese Hamster Ovary Cells
por: Li, Weifeng, et al.
Publicado: (2021)

Production of Recombinant Horseradish Peroxidase in an Engineered Cell-free Protein Synthesis System
por: Park, Yu-Jin, et al.
Publicado: (2021)

The Effect of microRNA on the Production of Recombinant Protein in CHO Cells and its Mechanism
por: Liu, Hui-Ning, et al.
Publicado: (2022)

Engineering of the Recombinant Expression and PEGylation Efficiency of the Therapeutic Enzyme Human Thymidine Phosphorylase
por: Karamitros, Christos S., et al.
Publicado: (2021)

Progress of Transposon Vector System for Production of Recombinant Therapeutic Proteins in Mammalian Cells
por: Wei, Mian, et al.
Publicado: (2022)

Design, Expression, Purification, and Characterization of a YFP-Tagged 2019-nCoV Spike Receptor-Binding Domain Construct
por: Bierig, Tobias, et al.
Publicado: (2020)

Effects of Oligosaccharides Isolated From Pinewood Hot Water Pre-hydrolyzates on Recombinant Cellulases
por: Fang, Hong, et al.
Publicado: (2018)

Cannot write session to /tmp/vufind_sessions/sess_1pjogfni6b745vd8tbtfldv7q5