Cargando…

The Effects of Maternal Smoking on Pregnancy and Offspring: Possible Role for EGF?

Cigarette smoke exposure during pregnancy and lactation is associated with adverse pregnancy outcomes. Here, we investigated the effects of maternal smoke exposure on pregnancy and offspring immunity and explored whether, epidermal growth factor (EGF), an important growth-promoting factor in human c...

Descripción completa

Detalles Bibliográficos
Autores principales: Janbazacyabar, Hamed, van Daal, Marthe, Leusink-Muis, Thea, van Ark, Ingrid, Garssen, Johan, Folkerts, Gert, van Bergenhenegouwen, Jeroen, Braber, Saskia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8415274/
https://www.ncbi.nlm.nih.gov/pubmed/34485278
http://dx.doi.org/10.3389/fcell.2021.680902
_version_ 1783747935950864384
author Janbazacyabar, Hamed
van Daal, Marthe
Leusink-Muis, Thea
van Ark, Ingrid
Garssen, Johan
Folkerts, Gert
van Bergenhenegouwen, Jeroen
Braber, Saskia
author_facet Janbazacyabar, Hamed
van Daal, Marthe
Leusink-Muis, Thea
van Ark, Ingrid
Garssen, Johan
Folkerts, Gert
van Bergenhenegouwen, Jeroen
Braber, Saskia
author_sort Janbazacyabar, Hamed
collection PubMed
description Cigarette smoke exposure during pregnancy and lactation is associated with adverse pregnancy outcomes. Here, we investigated the effects of maternal smoke exposure on pregnancy and offspring immunity and explored whether, epidermal growth factor (EGF), an important growth-promoting factor in human colostrum and milk, might be a possible missing link in maternal smoke exposure and changes in infants’ immune responses. Pregnant BALB/c mice were exposed to either cigarette smoke or air during gestation and lactation, and effects on pulmonary inflammation in dams and immune responses in offspring were examined. Maternal smoke exposure increased airway hyperresponsiveness and accumulation of inflammatory cells in the lungs of pregnant dams compared to non-pregnant dams. The E-cadherin protein expression was reduced in mammary glands of cigarette smoke-exposed pregnant dams. EGF levels were higher in mammary glands and serum of smoke-exposed pregnant dams compared to air-exposed pregnant dams. Offspring from cigarette smoke-exposed dams exhibited elevated levels of IL-17A, MCP-1, IL-22, and IL-13 in anti-CD3 stimulated spleen cell culture supernatants. EGF levels were also increased in serum of offspring from smoke-exposed dams. A positive correlation was observed between serum EGF levels and neutrophil numbers in bronchoalveolar lavage fluid of the dams. Interestingly, IL-17A, MCP-1, IL-22, IL13, and IFN-γ levels in anti-CD3 stimulated spleen cell culture supernatants of male pups also showed a positive correlation with EGF serum levels. In summary, our results reveal that maternal smoke exposure predisposes dams to exacerbated airway inflammation and offspring to exacerbated immune responses and both phenomena are associated with elevated EGF concentrations.
format Online
Article
Text
id pubmed-8415274
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-84152742021-09-04 The Effects of Maternal Smoking on Pregnancy and Offspring: Possible Role for EGF? Janbazacyabar, Hamed van Daal, Marthe Leusink-Muis, Thea van Ark, Ingrid Garssen, Johan Folkerts, Gert van Bergenhenegouwen, Jeroen Braber, Saskia Front Cell Dev Biol Cell and Developmental Biology Cigarette smoke exposure during pregnancy and lactation is associated with adverse pregnancy outcomes. Here, we investigated the effects of maternal smoke exposure on pregnancy and offspring immunity and explored whether, epidermal growth factor (EGF), an important growth-promoting factor in human colostrum and milk, might be a possible missing link in maternal smoke exposure and changes in infants’ immune responses. Pregnant BALB/c mice were exposed to either cigarette smoke or air during gestation and lactation, and effects on pulmonary inflammation in dams and immune responses in offspring were examined. Maternal smoke exposure increased airway hyperresponsiveness and accumulation of inflammatory cells in the lungs of pregnant dams compared to non-pregnant dams. The E-cadherin protein expression was reduced in mammary glands of cigarette smoke-exposed pregnant dams. EGF levels were higher in mammary glands and serum of smoke-exposed pregnant dams compared to air-exposed pregnant dams. Offspring from cigarette smoke-exposed dams exhibited elevated levels of IL-17A, MCP-1, IL-22, and IL-13 in anti-CD3 stimulated spleen cell culture supernatants. EGF levels were also increased in serum of offspring from smoke-exposed dams. A positive correlation was observed between serum EGF levels and neutrophil numbers in bronchoalveolar lavage fluid of the dams. Interestingly, IL-17A, MCP-1, IL-22, IL13, and IFN-γ levels in anti-CD3 stimulated spleen cell culture supernatants of male pups also showed a positive correlation with EGF serum levels. In summary, our results reveal that maternal smoke exposure predisposes dams to exacerbated airway inflammation and offspring to exacerbated immune responses and both phenomena are associated with elevated EGF concentrations. Frontiers Media S.A. 2021-08-16 /pmc/articles/PMC8415274/ /pubmed/34485278 http://dx.doi.org/10.3389/fcell.2021.680902 Text en Copyright © 2021 Janbazacyabar, van Daal, Leusink-Muis, van Ark, Garssen, Folkerts, van Bergenhenegouwen and Braber. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Janbazacyabar, Hamed
van Daal, Marthe
Leusink-Muis, Thea
van Ark, Ingrid
Garssen, Johan
Folkerts, Gert
van Bergenhenegouwen, Jeroen
Braber, Saskia
The Effects of Maternal Smoking on Pregnancy and Offspring: Possible Role for EGF?
title The Effects of Maternal Smoking on Pregnancy and Offspring: Possible Role for EGF?
title_full The Effects of Maternal Smoking on Pregnancy and Offspring: Possible Role for EGF?
title_fullStr The Effects of Maternal Smoking on Pregnancy and Offspring: Possible Role for EGF?
title_full_unstemmed The Effects of Maternal Smoking on Pregnancy and Offspring: Possible Role for EGF?
title_short The Effects of Maternal Smoking on Pregnancy and Offspring: Possible Role for EGF?
title_sort effects of maternal smoking on pregnancy and offspring: possible role for egf?
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8415274/
https://www.ncbi.nlm.nih.gov/pubmed/34485278
http://dx.doi.org/10.3389/fcell.2021.680902
work_keys_str_mv AT janbazacyabarhamed theeffectsofmaternalsmokingonpregnancyandoffspringpossibleroleforegf
AT vandaalmarthe theeffectsofmaternalsmokingonpregnancyandoffspringpossibleroleforegf
AT leusinkmuisthea theeffectsofmaternalsmokingonpregnancyandoffspringpossibleroleforegf
AT vanarkingrid theeffectsofmaternalsmokingonpregnancyandoffspringpossibleroleforegf
AT garssenjohan theeffectsofmaternalsmokingonpregnancyandoffspringpossibleroleforegf
AT folkertsgert theeffectsofmaternalsmokingonpregnancyandoffspringpossibleroleforegf
AT vanbergenhenegouwenjeroen theeffectsofmaternalsmokingonpregnancyandoffspringpossibleroleforegf
AT brabersaskia theeffectsofmaternalsmokingonpregnancyandoffspringpossibleroleforegf
AT janbazacyabarhamed effectsofmaternalsmokingonpregnancyandoffspringpossibleroleforegf
AT vandaalmarthe effectsofmaternalsmokingonpregnancyandoffspringpossibleroleforegf
AT leusinkmuisthea effectsofmaternalsmokingonpregnancyandoffspringpossibleroleforegf
AT vanarkingrid effectsofmaternalsmokingonpregnancyandoffspringpossibleroleforegf
AT garssenjohan effectsofmaternalsmokingonpregnancyandoffspringpossibleroleforegf
AT folkertsgert effectsofmaternalsmokingonpregnancyandoffspringpossibleroleforegf
AT vanbergenhenegouwenjeroen effectsofmaternalsmokingonpregnancyandoffspringpossibleroleforegf
AT brabersaskia effectsofmaternalsmokingonpregnancyandoffspringpossibleroleforegf