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CircCDYL Acts as a Tumor Suppressor in Wilms’ Tumor by Targeting miR-145-5p

Circular RNAs (circRNA) have been reported to exert evident functions in many human carcinomas. However, the possible mechanisms concerning the circRNA in various tumors are still elusive. In this research, we analyzed the expression profile and biological functions of circular RNA CDYL (circCDYL, c...

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Autores principales: Zhou, Rui, Jia, Wei, Gao, Xiaofeng, Deng, Fuming, Fu, Kai, Zhao, Tianxin, Li, Zhongmin, Fu, Wen, Liu, Guochang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8415843/
https://www.ncbi.nlm.nih.gov/pubmed/34485273
http://dx.doi.org/10.3389/fcell.2021.668947
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author Zhou, Rui
Jia, Wei
Gao, Xiaofeng
Deng, Fuming
Fu, Kai
Zhao, Tianxin
Li, Zhongmin
Fu, Wen
Liu, Guochang
author_facet Zhou, Rui
Jia, Wei
Gao, Xiaofeng
Deng, Fuming
Fu, Kai
Zhao, Tianxin
Li, Zhongmin
Fu, Wen
Liu, Guochang
author_sort Zhou, Rui
collection PubMed
description Circular RNAs (circRNA) have been reported to exert evident functions in many human carcinomas. However, the possible mechanisms concerning the circRNA in various tumors are still elusive. In this research, we analyzed the expression profile and biological functions of circular RNA CDYL (circCDYL, circBase ID: hsa_circ_0008285) in Wilms’ tumor. Here, miRNA and gene expression were examined by real-time PCR in Wilms’ tumor tissues and cell lines. The functions of circCDYL and its potential targets to influence cell proliferation, migration, and invasion in Wilms’ tumor cells were determined by biological functional experiments in vitro and in vivo. We predicted and analyzed potential miRNA targets through online bioinformatic tools. To validate the interactions between circCDYL and its targets, we performed RNA fluorescence in situ hybridization, biotin-coupled miRNA capture assay, and biotin-coupled probe pull-down assay. Tight junction protein l (TJP1) was proved to be the target gene of the predicted miRNA by dual-luciferase reporter assay. The expression level of TJP1 in Wilms’ tumor cells was identified via Western blot. We showed that circCDYL was downregulated in WT tissue compared with adjacent non-tumor tissue. Upregulation of circCDYL could reduce cell proliferation, migration, and invasion. Mechanically, circCDYL, functioning as a miRNA sponge, decreased the expression level of miR-145-5p and TJP1 3′UTR was validated as the target of miR-145-5p, facilitating the circCDYL/miR-145-5p/TJP1 axis. In conclusion, our study suggested circCDYL as a novel biomarker and therapeutic target for WT treatment.
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spelling pubmed-84158432021-09-04 CircCDYL Acts as a Tumor Suppressor in Wilms’ Tumor by Targeting miR-145-5p Zhou, Rui Jia, Wei Gao, Xiaofeng Deng, Fuming Fu, Kai Zhao, Tianxin Li, Zhongmin Fu, Wen Liu, Guochang Front Cell Dev Biol Cell and Developmental Biology Circular RNAs (circRNA) have been reported to exert evident functions in many human carcinomas. However, the possible mechanisms concerning the circRNA in various tumors are still elusive. In this research, we analyzed the expression profile and biological functions of circular RNA CDYL (circCDYL, circBase ID: hsa_circ_0008285) in Wilms’ tumor. Here, miRNA and gene expression were examined by real-time PCR in Wilms’ tumor tissues and cell lines. The functions of circCDYL and its potential targets to influence cell proliferation, migration, and invasion in Wilms’ tumor cells were determined by biological functional experiments in vitro and in vivo. We predicted and analyzed potential miRNA targets through online bioinformatic tools. To validate the interactions between circCDYL and its targets, we performed RNA fluorescence in situ hybridization, biotin-coupled miRNA capture assay, and biotin-coupled probe pull-down assay. Tight junction protein l (TJP1) was proved to be the target gene of the predicted miRNA by dual-luciferase reporter assay. The expression level of TJP1 in Wilms’ tumor cells was identified via Western blot. We showed that circCDYL was downregulated in WT tissue compared with adjacent non-tumor tissue. Upregulation of circCDYL could reduce cell proliferation, migration, and invasion. Mechanically, circCDYL, functioning as a miRNA sponge, decreased the expression level of miR-145-5p and TJP1 3′UTR was validated as the target of miR-145-5p, facilitating the circCDYL/miR-145-5p/TJP1 axis. In conclusion, our study suggested circCDYL as a novel biomarker and therapeutic target for WT treatment. Frontiers Media S.A. 2021-08-17 /pmc/articles/PMC8415843/ /pubmed/34485273 http://dx.doi.org/10.3389/fcell.2021.668947 Text en Copyright © 2021 Zhou, Jia, Gao, Deng, Fu, Zhao, Li, Fu and Liu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Zhou, Rui
Jia, Wei
Gao, Xiaofeng
Deng, Fuming
Fu, Kai
Zhao, Tianxin
Li, Zhongmin
Fu, Wen
Liu, Guochang
CircCDYL Acts as a Tumor Suppressor in Wilms’ Tumor by Targeting miR-145-5p
title CircCDYL Acts as a Tumor Suppressor in Wilms’ Tumor by Targeting miR-145-5p
title_full CircCDYL Acts as a Tumor Suppressor in Wilms’ Tumor by Targeting miR-145-5p
title_fullStr CircCDYL Acts as a Tumor Suppressor in Wilms’ Tumor by Targeting miR-145-5p
title_full_unstemmed CircCDYL Acts as a Tumor Suppressor in Wilms’ Tumor by Targeting miR-145-5p
title_short CircCDYL Acts as a Tumor Suppressor in Wilms’ Tumor by Targeting miR-145-5p
title_sort circcdyl acts as a tumor suppressor in wilms’ tumor by targeting mir-145-5p
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8415843/
https://www.ncbi.nlm.nih.gov/pubmed/34485273
http://dx.doi.org/10.3389/fcell.2021.668947
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