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Coiled-Coil Based Inclusion Bodies and Their Potential Applications
The production of recombinant proteins using microbial cell factories is frequently associated with the formation of inclusion bodies (IBs). These proteinaceous entities can be sometimes a reservoir of stable and active protein, might display good biocompatibility, and are produced efficiently and c...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2021
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8415879/ https://www.ncbi.nlm.nih.gov/pubmed/34485264 http://dx.doi.org/10.3389/fbioe.2021.734068 |
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author | Gil-Garcia, Marcos Ventura, Salvador |
author_facet | Gil-Garcia, Marcos Ventura, Salvador |
author_sort | Gil-Garcia, Marcos |
collection | PubMed |
description | The production of recombinant proteins using microbial cell factories is frequently associated with the formation of inclusion bodies (IBs). These proteinaceous entities can be sometimes a reservoir of stable and active protein, might display good biocompatibility, and are produced efficiently and cost-effectively. Thus, these submicrometric particles are increasingly exploited as functional biomaterials for biotechnological and biomedical purposes. The fusion of aggregation-prone sequences to the target protein is a successful strategy to sequester soluble recombinant polypeptides into IBs. Traditionally, the use of these IB-tags results in the formation of amyloid-like scaffolds where the protein of interest is trapped. This amyloid conformation might compromise the protein’s activity and be potentially cytotoxic. One promising alternative to overcome these limitations exploits the coiled-coil fold, composed of two or more α-helices and widely used by nature to create supramolecular assemblies. In this review, we summarize the state-of-the-art of functional IBs technology, focusing on the coiled-coil-assembly strategy, describing its advantages and applications, delving into future developments and necessary improvements in the field. |
format | Online Article Text |
id | pubmed-8415879 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-84158792021-09-04 Coiled-Coil Based Inclusion Bodies and Their Potential Applications Gil-Garcia, Marcos Ventura, Salvador Front Bioeng Biotechnol Bioengineering and Biotechnology The production of recombinant proteins using microbial cell factories is frequently associated with the formation of inclusion bodies (IBs). These proteinaceous entities can be sometimes a reservoir of stable and active protein, might display good biocompatibility, and are produced efficiently and cost-effectively. Thus, these submicrometric particles are increasingly exploited as functional biomaterials for biotechnological and biomedical purposes. The fusion of aggregation-prone sequences to the target protein is a successful strategy to sequester soluble recombinant polypeptides into IBs. Traditionally, the use of these IB-tags results in the formation of amyloid-like scaffolds where the protein of interest is trapped. This amyloid conformation might compromise the protein’s activity and be potentially cytotoxic. One promising alternative to overcome these limitations exploits the coiled-coil fold, composed of two or more α-helices and widely used by nature to create supramolecular assemblies. In this review, we summarize the state-of-the-art of functional IBs technology, focusing on the coiled-coil-assembly strategy, describing its advantages and applications, delving into future developments and necessary improvements in the field. Frontiers Media S.A. 2021-08-17 /pmc/articles/PMC8415879/ /pubmed/34485264 http://dx.doi.org/10.3389/fbioe.2021.734068 Text en Copyright © 2021 Gil-Garcia and Ventura. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Bioengineering and Biotechnology Gil-Garcia, Marcos Ventura, Salvador Coiled-Coil Based Inclusion Bodies and Their Potential Applications |
title | Coiled-Coil Based Inclusion Bodies and Their Potential Applications |
title_full | Coiled-Coil Based Inclusion Bodies and Their Potential Applications |
title_fullStr | Coiled-Coil Based Inclusion Bodies and Their Potential Applications |
title_full_unstemmed | Coiled-Coil Based Inclusion Bodies and Their Potential Applications |
title_short | Coiled-Coil Based Inclusion Bodies and Their Potential Applications |
title_sort | coiled-coil based inclusion bodies and their potential applications |
topic | Bioengineering and Biotechnology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8415879/ https://www.ncbi.nlm.nih.gov/pubmed/34485264 http://dx.doi.org/10.3389/fbioe.2021.734068 |
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