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Simultaneous Multiplex Genome Engineering via Accelerated Natural Transformation in Bacillus subtilis
Multiplex engineering at the scale of whole genomes has become increasingly important for synthetic biology and biotechnology applications. Although several methods have been reported for engineering microbe genomes, their use is limited by their complex procedures using multi-cycle transformations....
| Autores principales: | , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Frontiers Media S.A.
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8416114/ https://www.ncbi.nlm.nih.gov/pubmed/34484154 http://dx.doi.org/10.3389/fmicb.2021.714449 |