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Transcriptome Profiling Reveals B-Lineage Cells Contribute to the Poor Prognosis and Metastasis of Clear Cell Renal Cell Carcinoma

Although immune therapy can improve the treatment of clear cell renal cell carcinoma (ccRCC) significantly, there are still a large proportion of ccRCC patients who progress to metastasis. Targeting the pro-metastatic immune cell in the ccRCC microenvironment could provide a solution to this problem...

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Autores principales: Yang, Fafen, Zhao, Jingjie, Luo, Xiuzhuang, Li, Tong, Wang, Zechen, Wei, Qiuju, Lu, Heming, Meng, Yiliang, Cai, Kai, Lu, Liuying, Lu, Yushi, Chen, Lifen, Sooranna, Suren Rao, Luo, Linxue, Song, Jian, Meng, Lingzhang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8416254/
https://www.ncbi.nlm.nih.gov/pubmed/34485161
http://dx.doi.org/10.3389/fonc.2021.731896
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author Yang, Fafen
Zhao, Jingjie
Luo, Xiuzhuang
Li, Tong
Wang, Zechen
Wei, Qiuju
Lu, Heming
Meng, Yiliang
Cai, Kai
Lu, Liuying
Lu, Yushi
Chen, Lifen
Sooranna, Suren Rao
Luo, Linxue
Song, Jian
Meng, Lingzhang
author_facet Yang, Fafen
Zhao, Jingjie
Luo, Xiuzhuang
Li, Tong
Wang, Zechen
Wei, Qiuju
Lu, Heming
Meng, Yiliang
Cai, Kai
Lu, Liuying
Lu, Yushi
Chen, Lifen
Sooranna, Suren Rao
Luo, Linxue
Song, Jian
Meng, Lingzhang
author_sort Yang, Fafen
collection PubMed
description Although immune therapy can improve the treatment of clear cell renal cell carcinoma (ccRCC) significantly, there are still a large proportion of ccRCC patients who progress to metastasis. Targeting the pro-metastatic immune cell in the ccRCC microenvironment could provide a solution to this problem. In this study, B cells in ccRCC biopsies were identified by using scRNA-seq and flow cytometry. The findings indicated the presence of a pro-metastatic B cell type which could be further classified into 3 subpopulations, MARCH3, B2M and DTWD1, based on their large-scaled genetic profiles, rather than traditional Immature/Mature ones. Although all of the 3 subpopulations appeared to contribute to distant metastasis, B cell (B2M) was deemed to be the most essential. Moreover, STX16, CLASRP, ATIC, ACIN1 and SEMA4B, were genes found to be commonly up-regulated in the 3 subpopulations and this was correlated to a poor prognosis of ccRCC. Furthermore, the heterogeneity of plasma cells in ccRCC was also found to contribute to metastasis of the disease. This study offers potential novel therapeutic targets against distant metastasis of cancers, and can help to improve the therapeutic efficiency of ccRCC patients.
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spelling pubmed-84162542021-09-04 Transcriptome Profiling Reveals B-Lineage Cells Contribute to the Poor Prognosis and Metastasis of Clear Cell Renal Cell Carcinoma Yang, Fafen Zhao, Jingjie Luo, Xiuzhuang Li, Tong Wang, Zechen Wei, Qiuju Lu, Heming Meng, Yiliang Cai, Kai Lu, Liuying Lu, Yushi Chen, Lifen Sooranna, Suren Rao Luo, Linxue Song, Jian Meng, Lingzhang Front Oncol Oncology Although immune therapy can improve the treatment of clear cell renal cell carcinoma (ccRCC) significantly, there are still a large proportion of ccRCC patients who progress to metastasis. Targeting the pro-metastatic immune cell in the ccRCC microenvironment could provide a solution to this problem. In this study, B cells in ccRCC biopsies were identified by using scRNA-seq and flow cytometry. The findings indicated the presence of a pro-metastatic B cell type which could be further classified into 3 subpopulations, MARCH3, B2M and DTWD1, based on their large-scaled genetic profiles, rather than traditional Immature/Mature ones. Although all of the 3 subpopulations appeared to contribute to distant metastasis, B cell (B2M) was deemed to be the most essential. Moreover, STX16, CLASRP, ATIC, ACIN1 and SEMA4B, were genes found to be commonly up-regulated in the 3 subpopulations and this was correlated to a poor prognosis of ccRCC. Furthermore, the heterogeneity of plasma cells in ccRCC was also found to contribute to metastasis of the disease. This study offers potential novel therapeutic targets against distant metastasis of cancers, and can help to improve the therapeutic efficiency of ccRCC patients. Frontiers Media S.A. 2021-08-12 /pmc/articles/PMC8416254/ /pubmed/34485161 http://dx.doi.org/10.3389/fonc.2021.731896 Text en Copyright © 2021 Yang, Zhao, Luo, Li, Wang, Wei, Lu, Meng, Cai, Lu, Lu, Chen, Sooranna, Luo, Song and Meng https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Oncology
Yang, Fafen
Zhao, Jingjie
Luo, Xiuzhuang
Li, Tong
Wang, Zechen
Wei, Qiuju
Lu, Heming
Meng, Yiliang
Cai, Kai
Lu, Liuying
Lu, Yushi
Chen, Lifen
Sooranna, Suren Rao
Luo, Linxue
Song, Jian
Meng, Lingzhang
Transcriptome Profiling Reveals B-Lineage Cells Contribute to the Poor Prognosis and Metastasis of Clear Cell Renal Cell Carcinoma
title Transcriptome Profiling Reveals B-Lineage Cells Contribute to the Poor Prognosis and Metastasis of Clear Cell Renal Cell Carcinoma
title_full Transcriptome Profiling Reveals B-Lineage Cells Contribute to the Poor Prognosis and Metastasis of Clear Cell Renal Cell Carcinoma
title_fullStr Transcriptome Profiling Reveals B-Lineage Cells Contribute to the Poor Prognosis and Metastasis of Clear Cell Renal Cell Carcinoma
title_full_unstemmed Transcriptome Profiling Reveals B-Lineage Cells Contribute to the Poor Prognosis and Metastasis of Clear Cell Renal Cell Carcinoma
title_short Transcriptome Profiling Reveals B-Lineage Cells Contribute to the Poor Prognosis and Metastasis of Clear Cell Renal Cell Carcinoma
title_sort transcriptome profiling reveals b-lineage cells contribute to the poor prognosis and metastasis of clear cell renal cell carcinoma
topic Oncology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8416254/
https://www.ncbi.nlm.nih.gov/pubmed/34485161
http://dx.doi.org/10.3389/fonc.2021.731896
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