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Yiqi Huoxue Recipe Delayed Intervertebral Disc Degeneration by Activating Autophagy
Autophagy has been proved to occur in rats with intervertebral disc degeneration (IVDD). Yiqi Huoxue recipe (YQHXR), an effective therapy of traditional Chinese medicine, was widely used for ruptured lumbar disc herniation under clinical observation. More importantly, YQHXR positively regulated the...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2021
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8416448/ https://www.ncbi.nlm.nih.gov/pubmed/34483910 http://dx.doi.org/10.3389/fphar.2021.705747 |
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author | Dai, Feng Yu, Pengfei Yu, Zhenhan Jiang, Hong Ma, Zhijia Liu, Jintao |
author_facet | Dai, Feng Yu, Pengfei Yu, Zhenhan Jiang, Hong Ma, Zhijia Liu, Jintao |
author_sort | Dai, Feng |
collection | PubMed |
description | Autophagy has been proved to occur in rats with intervertebral disc degeneration (IVDD). Yiqi Huoxue recipe (YQHXR), an effective therapy of traditional Chinese medicine, was widely used for ruptured lumbar disc herniation under clinical observation. More importantly, YQHXR positively regulated the expression of autophagy-related proteins. However, little is known about the significance of YQHXR in the pathologic process of IVDD. Therefore, this study explored the protective effect of YQHXR based on IVDD rat model through magnetic resonance imaging and histopathologic analysis. Then we evaluated the formation of autophagosomes in the degenerated intervertebral disc by transmission electron microscope. Real-time PCR was used to detect the changes of autophagy-related genes. Western blot and immunoprecipitation were used to assess the protein expression of the autophagy-related pathway. We found that YQHXR-induced autophagy attenuated the release of inflammatory factors. In addition, YQHXR promoted the formation of Beclin1-VPS34 complex to activate autophagy through not only activation of the upstream protein AMPK and upregulation of the deubiquitinase USP13, thus in turn alleviating the development of IVDD. We proposed the potential molecular mechanism of YQHXR on autophagy for the first time, so as to provide a theoretical and experimental basis for the clinical application of YQHXR in the treatment of IVDD-related diseases. |
format | Online Article Text |
id | pubmed-8416448 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-84164482021-09-04 Yiqi Huoxue Recipe Delayed Intervertebral Disc Degeneration by Activating Autophagy Dai, Feng Yu, Pengfei Yu, Zhenhan Jiang, Hong Ma, Zhijia Liu, Jintao Front Pharmacol Pharmacology Autophagy has been proved to occur in rats with intervertebral disc degeneration (IVDD). Yiqi Huoxue recipe (YQHXR), an effective therapy of traditional Chinese medicine, was widely used for ruptured lumbar disc herniation under clinical observation. More importantly, YQHXR positively regulated the expression of autophagy-related proteins. However, little is known about the significance of YQHXR in the pathologic process of IVDD. Therefore, this study explored the protective effect of YQHXR based on IVDD rat model through magnetic resonance imaging and histopathologic analysis. Then we evaluated the formation of autophagosomes in the degenerated intervertebral disc by transmission electron microscope. Real-time PCR was used to detect the changes of autophagy-related genes. Western blot and immunoprecipitation were used to assess the protein expression of the autophagy-related pathway. We found that YQHXR-induced autophagy attenuated the release of inflammatory factors. In addition, YQHXR promoted the formation of Beclin1-VPS34 complex to activate autophagy through not only activation of the upstream protein AMPK and upregulation of the deubiquitinase USP13, thus in turn alleviating the development of IVDD. We proposed the potential molecular mechanism of YQHXR on autophagy for the first time, so as to provide a theoretical and experimental basis for the clinical application of YQHXR in the treatment of IVDD-related diseases. Frontiers Media S.A. 2021-08-18 /pmc/articles/PMC8416448/ /pubmed/34483910 http://dx.doi.org/10.3389/fphar.2021.705747 Text en Copyright © 2021 Dai, Yu, Yu, Jiang, Ma and Liu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Pharmacology Dai, Feng Yu, Pengfei Yu, Zhenhan Jiang, Hong Ma, Zhijia Liu, Jintao Yiqi Huoxue Recipe Delayed Intervertebral Disc Degeneration by Activating Autophagy |
title | Yiqi Huoxue Recipe Delayed Intervertebral Disc Degeneration by Activating Autophagy |
title_full | Yiqi Huoxue Recipe Delayed Intervertebral Disc Degeneration by Activating Autophagy |
title_fullStr | Yiqi Huoxue Recipe Delayed Intervertebral Disc Degeneration by Activating Autophagy |
title_full_unstemmed | Yiqi Huoxue Recipe Delayed Intervertebral Disc Degeneration by Activating Autophagy |
title_short | Yiqi Huoxue Recipe Delayed Intervertebral Disc Degeneration by Activating Autophagy |
title_sort | yiqi huoxue recipe delayed intervertebral disc degeneration by activating autophagy |
topic | Pharmacology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8416448/ https://www.ncbi.nlm.nih.gov/pubmed/34483910 http://dx.doi.org/10.3389/fphar.2021.705747 |
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