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Exploring Amyloidogenicity of Peptides From Ribosomal S1 Protein to Develop Novel AMPs

Antimicrobial peptides (AMPs) and similar compounds are potential candidates for combating antibiotic-resistant bacteria. The hypothesis of directed co-aggregation of the target protein and an amyloidogenic peptide acting as an antimicrobial peptide was successfully tested for peptides synthesized o...

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Autor principal: Galzitskaya, Oxana V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8416663/
https://www.ncbi.nlm.nih.gov/pubmed/34490350
http://dx.doi.org/10.3389/fmolb.2021.705069
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author Galzitskaya, Oxana V.
author_facet Galzitskaya, Oxana V.
author_sort Galzitskaya, Oxana V.
collection PubMed
description Antimicrobial peptides (AMPs) and similar compounds are potential candidates for combating antibiotic-resistant bacteria. The hypothesis of directed co-aggregation of the target protein and an amyloidogenic peptide acting as an antimicrobial peptide was successfully tested for peptides synthesized on the basis of ribosomal S1 protein in the bacterial culture of T. thermophilus. Co-aggregation of the target protein and amyloidogenic peptide was also tested for the pathogenic ribosomal S1 protein from P. aeruginosa. Almost all peptides that we selected as AMPs, prone to aggregation and formation of fibrils, based on the amino acid sequence of ribosomal S1 protein from E. coli, T. thermophilus, P. aeruginosa, formed amyloid fibrils. We have demonstrated that amyloidogenic peptides are not only toxic to their target cells, but also some of them have antimicrobial activity. Controlling the aggregation of vital bacterial proteins can become one of the new directions of research and form the basis for the search and development of targeted antibacterial drugs.
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spelling pubmed-84166632021-09-05 Exploring Amyloidogenicity of Peptides From Ribosomal S1 Protein to Develop Novel AMPs Galzitskaya, Oxana V. Front Mol Biosci Molecular Biosciences Antimicrobial peptides (AMPs) and similar compounds are potential candidates for combating antibiotic-resistant bacteria. The hypothesis of directed co-aggregation of the target protein and an amyloidogenic peptide acting as an antimicrobial peptide was successfully tested for peptides synthesized on the basis of ribosomal S1 protein in the bacterial culture of T. thermophilus. Co-aggregation of the target protein and amyloidogenic peptide was also tested for the pathogenic ribosomal S1 protein from P. aeruginosa. Almost all peptides that we selected as AMPs, prone to aggregation and formation of fibrils, based on the amino acid sequence of ribosomal S1 protein from E. coli, T. thermophilus, P. aeruginosa, formed amyloid fibrils. We have demonstrated that amyloidogenic peptides are not only toxic to their target cells, but also some of them have antimicrobial activity. Controlling the aggregation of vital bacterial proteins can become one of the new directions of research and form the basis for the search and development of targeted antibacterial drugs. Frontiers Media S.A. 2021-08-19 /pmc/articles/PMC8416663/ /pubmed/34490350 http://dx.doi.org/10.3389/fmolb.2021.705069 Text en Copyright © 2021 Galzitskaya. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Molecular Biosciences
Galzitskaya, Oxana V.
Exploring Amyloidogenicity of Peptides From Ribosomal S1 Protein to Develop Novel AMPs
title Exploring Amyloidogenicity of Peptides From Ribosomal S1 Protein to Develop Novel AMPs
title_full Exploring Amyloidogenicity of Peptides From Ribosomal S1 Protein to Develop Novel AMPs
title_fullStr Exploring Amyloidogenicity of Peptides From Ribosomal S1 Protein to Develop Novel AMPs
title_full_unstemmed Exploring Amyloidogenicity of Peptides From Ribosomal S1 Protein to Develop Novel AMPs
title_short Exploring Amyloidogenicity of Peptides From Ribosomal S1 Protein to Develop Novel AMPs
title_sort exploring amyloidogenicity of peptides from ribosomal s1 protein to develop novel amps
topic Molecular Biosciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8416663/
https://www.ncbi.nlm.nih.gov/pubmed/34490350
http://dx.doi.org/10.3389/fmolb.2021.705069
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