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Exploring Amyloidogenicity of Peptides From Ribosomal S1 Protein to Develop Novel AMPs
Antimicrobial peptides (AMPs) and similar compounds are potential candidates for combating antibiotic-resistant bacteria. The hypothesis of directed co-aggregation of the target protein and an amyloidogenic peptide acting as an antimicrobial peptide was successfully tested for peptides synthesized o...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2021
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8416663/ https://www.ncbi.nlm.nih.gov/pubmed/34490350 http://dx.doi.org/10.3389/fmolb.2021.705069 |
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author | Galzitskaya, Oxana V. |
author_facet | Galzitskaya, Oxana V. |
author_sort | Galzitskaya, Oxana V. |
collection | PubMed |
description | Antimicrobial peptides (AMPs) and similar compounds are potential candidates for combating antibiotic-resistant bacteria. The hypothesis of directed co-aggregation of the target protein and an amyloidogenic peptide acting as an antimicrobial peptide was successfully tested for peptides synthesized on the basis of ribosomal S1 protein in the bacterial culture of T. thermophilus. Co-aggregation of the target protein and amyloidogenic peptide was also tested for the pathogenic ribosomal S1 protein from P. aeruginosa. Almost all peptides that we selected as AMPs, prone to aggregation and formation of fibrils, based on the amino acid sequence of ribosomal S1 protein from E. coli, T. thermophilus, P. aeruginosa, formed amyloid fibrils. We have demonstrated that amyloidogenic peptides are not only toxic to their target cells, but also some of them have antimicrobial activity. Controlling the aggregation of vital bacterial proteins can become one of the new directions of research and form the basis for the search and development of targeted antibacterial drugs. |
format | Online Article Text |
id | pubmed-8416663 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-84166632021-09-05 Exploring Amyloidogenicity of Peptides From Ribosomal S1 Protein to Develop Novel AMPs Galzitskaya, Oxana V. Front Mol Biosci Molecular Biosciences Antimicrobial peptides (AMPs) and similar compounds are potential candidates for combating antibiotic-resistant bacteria. The hypothesis of directed co-aggregation of the target protein and an amyloidogenic peptide acting as an antimicrobial peptide was successfully tested for peptides synthesized on the basis of ribosomal S1 protein in the bacterial culture of T. thermophilus. Co-aggregation of the target protein and amyloidogenic peptide was also tested for the pathogenic ribosomal S1 protein from P. aeruginosa. Almost all peptides that we selected as AMPs, prone to aggregation and formation of fibrils, based on the amino acid sequence of ribosomal S1 protein from E. coli, T. thermophilus, P. aeruginosa, formed amyloid fibrils. We have demonstrated that amyloidogenic peptides are not only toxic to their target cells, but also some of them have antimicrobial activity. Controlling the aggregation of vital bacterial proteins can become one of the new directions of research and form the basis for the search and development of targeted antibacterial drugs. Frontiers Media S.A. 2021-08-19 /pmc/articles/PMC8416663/ /pubmed/34490350 http://dx.doi.org/10.3389/fmolb.2021.705069 Text en Copyright © 2021 Galzitskaya. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Molecular Biosciences Galzitskaya, Oxana V. Exploring Amyloidogenicity of Peptides From Ribosomal S1 Protein to Develop Novel AMPs |
title | Exploring Amyloidogenicity of Peptides From Ribosomal S1 Protein to Develop Novel AMPs |
title_full | Exploring Amyloidogenicity of Peptides From Ribosomal S1 Protein to Develop Novel AMPs |
title_fullStr | Exploring Amyloidogenicity of Peptides From Ribosomal S1 Protein to Develop Novel AMPs |
title_full_unstemmed | Exploring Amyloidogenicity of Peptides From Ribosomal S1 Protein to Develop Novel AMPs |
title_short | Exploring Amyloidogenicity of Peptides From Ribosomal S1 Protein to Develop Novel AMPs |
title_sort | exploring amyloidogenicity of peptides from ribosomal s1 protein to develop novel amps |
topic | Molecular Biosciences |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8416663/ https://www.ncbi.nlm.nih.gov/pubmed/34490350 http://dx.doi.org/10.3389/fmolb.2021.705069 |
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