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Identification of Circular RNA Expression Profiles in White Adipocytes and Their Roles in Adipogenesis

Obesity and its related metabolic diseases have become great public health threats worldwide. Although accumulated evidence suggests that circRNA is a new type of non-coding RNAs regulating various physiological and pathological processes, little attention has been paid to the expression profiles an...

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Autores principales: Zhang, Peng-peng, Han, Qiu, Sheng, Ming-xuan, Du, Chun-yu, Wang, Ya-ling, Cheng, Xiao-fang, Xu, Hai-xia, Li, Cen-cen, Xu, Yong-jie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8417237/
https://www.ncbi.nlm.nih.gov/pubmed/34489740
http://dx.doi.org/10.3389/fphys.2021.728208
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author Zhang, Peng-peng
Han, Qiu
Sheng, Ming-xuan
Du, Chun-yu
Wang, Ya-ling
Cheng, Xiao-fang
Xu, Hai-xia
Li, Cen-cen
Xu, Yong-jie
author_facet Zhang, Peng-peng
Han, Qiu
Sheng, Ming-xuan
Du, Chun-yu
Wang, Ya-ling
Cheng, Xiao-fang
Xu, Hai-xia
Li, Cen-cen
Xu, Yong-jie
author_sort Zhang, Peng-peng
collection PubMed
description Obesity and its related metabolic diseases have become great public health threats worldwide. Although accumulated evidence suggests that circRNA is a new type of non-coding RNAs regulating various physiological and pathological processes, little attention has been paid to the expression profiles and functions of circRNAs in white adipose tissue. In this study, 3,771 circRNAs were detected in three stages of white adipogenesis (preadipocyte, differentiating preadipocyte, and mature adipocyte) by RNA-seq. Experimental validation suggested that the RNA-seq results are highly reliable. We found that nearly 10% of genes which expressed linear RNAs in adipocytes could also generate circRNAs. In addition, 40% of them produced multiple circRNA isoforms. We performed correlation analysis and found that a great deal of circRNAs (nearly 50%) and their parental genes were highly correlated in expression levels. A total of 41 differential expression circRNAs (DECs) were detected during adipogenesis and an extremely high ratio of them (80%) were correlated with their parental genes, indicating these circRNAs may potentially play roles in regulating the expression of their parental genes. KEGG enrichment and GO annotation of the parental genes suggesting that the DECs may participate in several adipogenesis-related pathways. Following rigorous selection, we found that many up-regulated circRNAs contain multiple miRNAs binding sites, such as miR17, miR-30c, and miR-130, indicating they may potentially facilitate their regulatory functions by acting as miRNA sponges. These results suggest that plenty of circRNAs are expressed in white adipogenesis and the DECs may serve as new candidates for future adipogenesis regulation.
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spelling pubmed-84172372021-09-05 Identification of Circular RNA Expression Profiles in White Adipocytes and Their Roles in Adipogenesis Zhang, Peng-peng Han, Qiu Sheng, Ming-xuan Du, Chun-yu Wang, Ya-ling Cheng, Xiao-fang Xu, Hai-xia Li, Cen-cen Xu, Yong-jie Front Physiol Physiology Obesity and its related metabolic diseases have become great public health threats worldwide. Although accumulated evidence suggests that circRNA is a new type of non-coding RNAs regulating various physiological and pathological processes, little attention has been paid to the expression profiles and functions of circRNAs in white adipose tissue. In this study, 3,771 circRNAs were detected in three stages of white adipogenesis (preadipocyte, differentiating preadipocyte, and mature adipocyte) by RNA-seq. Experimental validation suggested that the RNA-seq results are highly reliable. We found that nearly 10% of genes which expressed linear RNAs in adipocytes could also generate circRNAs. In addition, 40% of them produced multiple circRNA isoforms. We performed correlation analysis and found that a great deal of circRNAs (nearly 50%) and their parental genes were highly correlated in expression levels. A total of 41 differential expression circRNAs (DECs) were detected during adipogenesis and an extremely high ratio of them (80%) were correlated with their parental genes, indicating these circRNAs may potentially play roles in regulating the expression of their parental genes. KEGG enrichment and GO annotation of the parental genes suggesting that the DECs may participate in several adipogenesis-related pathways. Following rigorous selection, we found that many up-regulated circRNAs contain multiple miRNAs binding sites, such as miR17, miR-30c, and miR-130, indicating they may potentially facilitate their regulatory functions by acting as miRNA sponges. These results suggest that plenty of circRNAs are expressed in white adipogenesis and the DECs may serve as new candidates for future adipogenesis regulation. Frontiers Media S.A. 2021-08-19 /pmc/articles/PMC8417237/ /pubmed/34489740 http://dx.doi.org/10.3389/fphys.2021.728208 Text en Copyright © 2021 Zhang, Han, Sheng, Du, Wang, Cheng, Xu, Li and Xu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Physiology
Zhang, Peng-peng
Han, Qiu
Sheng, Ming-xuan
Du, Chun-yu
Wang, Ya-ling
Cheng, Xiao-fang
Xu, Hai-xia
Li, Cen-cen
Xu, Yong-jie
Identification of Circular RNA Expression Profiles in White Adipocytes and Their Roles in Adipogenesis
title Identification of Circular RNA Expression Profiles in White Adipocytes and Their Roles in Adipogenesis
title_full Identification of Circular RNA Expression Profiles in White Adipocytes and Their Roles in Adipogenesis
title_fullStr Identification of Circular RNA Expression Profiles in White Adipocytes and Their Roles in Adipogenesis
title_full_unstemmed Identification of Circular RNA Expression Profiles in White Adipocytes and Their Roles in Adipogenesis
title_short Identification of Circular RNA Expression Profiles in White Adipocytes and Their Roles in Adipogenesis
title_sort identification of circular rna expression profiles in white adipocytes and their roles in adipogenesis
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8417237/
https://www.ncbi.nlm.nih.gov/pubmed/34489740
http://dx.doi.org/10.3389/fphys.2021.728208
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